======= GPI =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GPI
  * **<color #00a2e8>Official Name</color>**: glucose-6-phosphate isomerase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2821|2821]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P06744|P06744]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GPI&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GPI|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/172400|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. {ECO:0000269|PubMed:11004567, ECO:0000269|PubMed:11437381}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PGI|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|glucose-6-phosphate isomerase activity|
|intramolecular transferase activity|
|positive regulation of immunoglobulin secretion|
|monosaccharide binding|
|regulation of immunoglobulin secretion|
|glucose 6-phosphate metabolic process|
|canonical glycolysis|
|NADH regeneration|
|glucose catabolic process to pyruvate|
|glycolytic process through fructose-6-phosphate|
|glycolytic process through glucose-6-phosphate|
|glucose catabolic process|
|NADH metabolic process|
|glycolytic process|
|ATP generation from ADP|
|positive regulation of immunoglobulin production|
|gluconeogenesis|
|NAD metabolic process|
|hexose biosynthetic process|
|ADP metabolic process|
|hexose catabolic process|
|monosaccharide biosynthetic process|
|nucleoside diphosphate phosphorylation|
|purine nucleoside diphosphate metabolic process|
|monosaccharide catabolic process|
|purine ribonucleoside diphosphate metabolic process|
|nucleotide phosphorylation|
|ribonucleoside diphosphate metabolic process|
|regulation of immunoglobulin production|
|pyruvate metabolic process|
|nucleoside diphosphate metabolic process|
|positive regulation of production of molecular mediator of immune response|
|positive regulation of endothelial cell migration|
|glucose metabolic process|
|secretory granule lumen|
|carbohydrate catabolic process|
|ficolin-1-rich granule lumen|
|carbohydrate biosynthetic process|
|regulation of production of molecular mediator of immune response|
|positive regulation of epithelial cell migration|
|hexose metabolic process|
|regulation of endothelial cell migration|
|growth factor activity|
|cytokine activity|
|monosaccharide metabolic process|
|ATP metabolic process|
|positive regulation of immune effector process|
|regulation of epithelial cell migration|
|positive regulation of protein secretion|
|positive regulation of peptide secretion|
|ubiquitin protein ligase binding|
|hemostasis|
|purine ribonucleotide metabolic process|
|ribonucleotide metabolic process|
|purine nucleotide metabolic process|
|ribose phosphate metabolic process|
|humoral immune response|
|purine-containing compound metabolic process|
|positive regulation of secretion by cell|
|generation of precursor metabolites and energy|
|positive regulation of protein transport|
|positive regulation of secretion|
|small molecule catabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|positive regulation of establishment of protein localization|
|regulation of protein secretion|
|regulation of immune effector process|
|carbohydrate metabolic process|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|regulation of peptide secretion|
|regulation of body fluid levels|
|neutrophil mediated immunity|
|positive regulation of cell migration|
|neutrophil activation|
|granulocyte activation|
|leukocyte degranulation|
|myeloid leukocyte mediated immunity|
|positive regulation of cell motility|
|myeloid cell activation involved in immune response|
|monocarboxylic acid metabolic process|
|positive regulation of cellular component movement|
|nucleobase-containing small molecule metabolic process|
|positive regulation of locomotion|
|myeloid leukocyte activation|
|small molecule biosynthetic process|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|regulated exocytosis|
|regulation of protein transport|
|regulation of peptide transport|
|regulation of establishment of protein localization|
|regulation of secretion by cell|
|leukocyte mediated immunity|
|exocytosis|
|regulation of secretion|
|regulation of cell migration|
|organophosphate metabolic process|
|carboxylic acid metabolic process|
|regulation of cell motility|
|leukocyte activation|
|oxidation-reduction process|
|regulation of locomotion|
|positive regulation of transport|
|regulation of cellular component movement|
|oxoacid metabolic process|
|secretion by cell|
|organic acid metabolic process|
|regulation of protein localization|
|carbohydrate derivative metabolic process|
|export from cell|
|cell activation|
|immune effector process|
|secretion|
|positive regulation of immune system process|
|phosphorylation|
|regulation of immune system process|
|positive regulation of multicellular organismal process|
|small molecule metabolic process|
|organic substance catabolic process|
|regulation of transport|
|immune response|
|extracellular region|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp438|NN-Diethyl-meta-toluamide 500μM R08 exp438]]|-3.02|
|[[:results:exp380|NMS-873 0.07μM R07 exp380]]|-2.15|
|[[:results:exp222|Betulinic acid 10 to 15μM on day4 R05 exp222]]|-2.11|
|[[:results:exp534|Trientine 500μM R08 exp534]]|-1.9|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|-1.89|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|-1.87|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|-1.82|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|-1.76|
|[[:results:exp480|ETC-159 50μM R08 exp480]]|-1.74|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|-1.73|
|[[:results:exp153|SGC2096 2.6μM R03 exp153]]|-1.71|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|1.76|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|1.77|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|1.79|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|1.79|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|1.81|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.89|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|1.9|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|1.91|
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|1.96|
|[[:results:exp539|42°C R08 exp539]]|1.97|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|2|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|2.08|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|2.15|
|[[:results:exp211|AICAR 240μM R05 exp211]]|2.27|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|2.57|
|[[:results:exp360|Genistein 15μM R07 exp360]]|2.93|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|2.97|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|3.63|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|3.84|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hk2|HK2]]|0.624|
|[[:human genes:p:pgp|PGP]]|0.578|
|[[:human genes:s:slc2a1|SLC2A1]]|0.538|
|[[:human genes:p:pgls|PGLS]]|0.514|
|[[:human genes:p:pfkp|PFKP]]|0.512|
|[[:human genes:c:crls1|CRLS1]]|0.436|
|[[:human genes:a:ahcyl1|AHCYL1]]|0.424|
|[[:human genes:t:tkt|TKT]]|0.422|
|[[:human genes:r:romo1|ROMO1]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 39/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|1/28|
|blood|1/28|
|bone|1/26|
|breast|0/33|
|central nervous system|3/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|2/16|
|kidney|4/21|
|liver|2/20|
|lung|5/75|
|lymphocyte|0/16|
|ovary|1/26|
|pancreas|1/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|1/9|
|thyroid|0/2|
|upper aerodigestive|2/22|
|urinary tract|2/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 37
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.18 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GPI Expression in NALM6 Cells: 8.18'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>