======= H2AFX =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: H2AX
  * **<color #00a2e8>Official Name</color>**: H2A.X variant histone
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=3014|3014]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P16104|P16104]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=H2AFX&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20H2AFX|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601772|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. {ECO:0000269|PubMed:10959836, ECO:0000269|PubMed:12419185, ECO:0000269|PubMed:12607005, ECO:0000269|PubMed:15201865}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Histone|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|XY body|
|male germ cell nucleus|
|site of DNA damage|
|replication fork|
|condensed nuclear chromosome|
|cellular response to gamma radiation|
|cellular senescence|
|chromosome, telomeric region|
|response to gamma radiation|
|damaged DNA binding|
|site of double-strand break|
|double-strand break repair via nonhomologous end joining|
|cell aging|
|non-recombinational repair|
|positive regulation of DNA repair|
|nucleosome|
|cellular response to ionizing radiation|
|double-strand break repair via homologous recombination|
|positive regulation of response to DNA damage stimulus|
|recombinational repair|
|cerebral cortex development|
|nucleosome assembly|
|regulation of DNA repair|
|histone binding|
|DNA damage checkpoint|
|chromatin assembly|
|DNA integrity checkpoint|
|response to ionizing radiation|
|chromatin assembly or disassembly|
|nucleosome organization|
|pallium development|
|cellular response to radiation|
|DNA packaging|
|double-strand break repair|
|cell cycle checkpoint|
|positive regulation of DNA metabolic process|
|protein-DNA complex assembly|
|regulation of response to DNA damage stimulus|
|DNA recombination|
|meiotic cell cycle|
|nuclear chromatin|
|protein-DNA complex subunit organization|
|telencephalon development|
|aging|
|DNA conformation change|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|enzyme binding|
|regulation of DNA metabolic process|
|forebrain development|
|nuclear speck|
|response to radiation|
|protein heterodimerization activity|
|centrosome|
|DNA repair|
|spermatogenesis|
|male gamete generation|
|negative regulation of cell cycle|
|gamete generation|
|chromatin organization|
|viral process|
|regulation of cellular response to stress|
|brain development|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|head development|
|symbiotic process|
|interspecies interaction between organisms|
|multicellular organismal reproductive process|
|sexual reproduction|
|cellular protein-containing complex assembly|
|multicellular organism reproduction|
|central nervous system development|
|multi-organism reproductive process|
|chromosome organization|
|response to abiotic stimulus|
|regulation of cell cycle|
|cell cycle|
|reproductive process|
|reproduction|
|DNA binding|
|regulation of response to stress|
|protein-containing complex assembly|
|cellular response to stress|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|-3|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-2.79|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.73|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-1.85|
|[[:results:exp300|VE-822 0.04μM R06 exp300]]|-1.82|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|-1.79|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rnf168|RNF168]]|0.515|
|[[:human genes:s:swsap1|SWSAP1]]|0.462|
|[[:human genes:s:spidr|SPIDR]]|0.449|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 44/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|2/28|
|bone|2/26|
|breast|4/33|
|central nervous system|2/56|
|cervix|1/4|
|colorectal|0/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|1/16|
|kidney|3/21|
|liver|3/20|
|lung|1/75|
|lymphocyte|2/16|
|ovary|4/26|
|pancreas|1/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|2/22|
|urinary tract|4/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1288
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.37 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='H2AFX Expression in NALM6 Cells: 6.37'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>