======= HLCS =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: HLCS
  * **<color #00a2e8>Official Name</color>**: holocarboxylase synthetase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=3141|3141]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P50747|P50747]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=HLCS&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20HLCS|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/609018|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl- CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|BPL C|
|BPL LplA LipB|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity|
|protein biotinylation|
|biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity|
|histone biotinylation|
|biotin-protein ligase activity|
|biotin-[acetyl-CoA-carboxylase] ligase activity|
|biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity|
|response to biotin|
|biotin binding|
|nuclear lamina|
|biotin metabolic process|
|response to vitamin|
|water-soluble vitamin metabolic process|
|nuclear matrix|
|chromatin|
|vitamin metabolic process|
|response to nutrient|
|coenzyme metabolic process|
|response to acid chemical|
|enzyme binding|
|histone modification|
|sulfur compound metabolic process|
|covalent chromatin modification|
|cofactor metabolic process|
|response to nutrient levels|
|drug metabolic process|
|monocarboxylic acid metabolic process|
|response to extracellular stimulus|
|cell population proliferation|
|chromatin organization|
|cellular amide metabolic process|
|protein homodimerization activity|
|carboxylic acid metabolic process|
|response to organic cyclic compound|
|response to organonitrogen compound|
|oxoacid metabolic process|
|response to drug|
|organic acid metabolic process|
|chromosome organization|
|response to nitrogen compound|
|mitochondrion|
|ATP binding|
|response to oxygen-containing compound|
|small molecule metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|-1.86|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|-1.74|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|1.87|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|1.97|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|2.04|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|2.23|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5691
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.02 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='HLCS Expression in NALM6 Cells: 4.02'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>