======= MCM8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MCM8
  * **<color #00a2e8>Official Name</color>**: minichromosome maintenance 8 homologous recombination repair factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=84515|84515]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UJA3|Q9UJA3]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MCM8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MCM8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608187|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|MCM|
|AAA 5|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|recombinational interstrand cross-link repair|
|MCM8-MCM9 complex|
|MutLbeta complex binding|
|MutSbeta complex binding|
|MutSalpha complex binding|
|MCM complex|
|protein localization to chromatin|
|DNA replication origin binding|
|interstrand cross-link repair|
|DNA helicase activity|
|protein localization to chromosome|
|double-strand break repair via homologous recombination|
|recombinational repair|
|single-stranded DNA binding|
|DNA duplex unwinding|
|DNA geometric change|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|chromosome|
|female gamete generation|
|protein stabilization|
|double-strand break repair|
|DNA replication|
|DNA recombination|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|regulation of protein stability|
|DNA conformation change|
|enzyme binding|
|chromatin binding|
|DNA repair|
|male gamete generation|
|mitotic cell cycle process|
|mitotic cell cycle|
|gamete generation|
|protein localization to organelle|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|multicellular organismal reproductive process|
|sexual reproduction|
|multicellular organism reproduction|
|multi-organism reproductive process|
|cell cycle process|
|chromosome organization|
|cell cycle|
|reproductive process|
|reproduction|
|ATP binding|
|cellular protein localization|
|cellular macromolecule localization|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-3.3|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.72|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-2.3|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-2.23|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|-2.1|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-2.05|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-2.02|
|[[:results:exp219|A-395N 10μM R05 exp219]]|-2|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-1.97|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-1.93|
|[[:results:exp170|Metformin 100 to 150μM on day4 R04 exp170]]|-1.89|
|[[:results:exp53|Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53]]|-1.89|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-1.86|
|[[:results:exp437|Ethanol 0.003 R08 exp437]]|-1.8|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|-1.79|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-1.79|
|[[:results:exp59|UMK57 1μM R01 exp59]]|-1.75|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-1.71|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|1.75|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mcm9|MCM9]]|0.559|
|[[:human genes:s:sfr1|SFR1]]|0.449|
|[[:human genes:p:ptchd3|PTCHD3]]|0.421|
|[[:human genes:x:xrcc2|XRCC2]]|0.418|
|[[:human genes:r:rad51b|RAD51B]]|0.41|
|[[:human genes:m:mad2l2|MAD2L2]]|0.406|
|[[:human genes:r:rnaseh2b|RNASEH2B]]|0.404|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 4791
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.3 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MCM8 Expression in NALM6 Cells: 7.3'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>