======= MGMT =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MGMT
  * **<color #00a2e8>Official Name</color>**: O-6-methylguanine-DNA methyltransferase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4255|4255]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P16455|P16455]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MGMT&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MGMT|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/156569|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DNA binding 1|
|Methyltransf 1N|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|methylated-DNA-[protein]-cysteine S-methyltransferase activity|
|DNA-methyltransferase activity|
|DNA dealkylation involved in DNA repair|
|response to folic acid|
|DNA ligation|
|mammary gland epithelial cell differentiation|
|DNA dealkylation|
|positive regulation of double-strand break repair|
|DNA alkylation|
|DNA methylation|
|methyltransferase activity|
|mammary gland epithelium development|
|DNA methylation or demethylation|
|positive regulation of DNA repair|
|cellular response to ionizing radiation|
|regulation of double-strand break repair|
|response to vitamin|
|DNA modification|
|positive regulation of response to DNA damage stimulus|
|regulation of DNA repair|
|response to ethanol|
|mammary gland development|
|response to ionizing radiation|
|cellular response to radiation|
|positive regulation of DNA metabolic process|
|response to nutrient|
|regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|regulation of response to DNA damage stimulus|
|response to alcohol|
|cellular response to oxidative stress|
|regulation of cysteine-type endopeptidase activity|
|macromolecule methylation|
|response to antibiotic|
|methylation|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|response to acid chemical|
|regulation of DNA metabolic process|
|response to oxidative stress|
|gland development|
|regulation of endopeptidase activity|
|response to radiation|
|regulation of peptidase activity|
|response to nutrient levels|
|response to toxic substance|
|DNA repair|
|response to extracellular stimulus|
|cellular response to organic cyclic compound|
|epithelial cell differentiation|
|calcium ion binding|
|regulation of proteolysis|
|regulation of cellular response to stress|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|response to organic cyclic compound|
|negative regulation of cell death|
|response to organonitrogen compound|
|response to drug|
|response to nitrogen compound|
|epithelium development|
|response to abiotic stimulus|
|regulation of hydrolase activity|
|DNA binding|
|regulation of response to stress|
|regulation of apoptotic process|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|regulation of cell death|
|cellular response to stress|
|tissue development|
|positive regulation of nucleobase-containing compound metabolic process|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp75|MK-1775 0.32μM R02 exp75]]|1.87|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11536
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 3.83 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MGMT Expression in NALM6 Cells: 3.83'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>