======= MRE11A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MRE11
  * **<color #00a2e8>Official Name</color>**: MRE11 homolog, double strand break repair nuclease
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4361|4361]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P49959|P49959]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MRE11A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MRE11A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600814|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). {ECO:0000269|PubMed:10888888, ECO:0000269|PubMed:11741547, ECO:0000269|PubMed:15064416, ECO:0000269|PubMed:9590181, ECO:0000269|PubMed:9705271}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Metallophos 2|
|Mre11 DNA bind|
|Metallophos|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|mitochondrial double-strand break repair|
|mitochondrial double-strand break repair via homologous recombination|
|DNA strand resection involved in replication fork processing|
|telomeric 3 overhang formation|
|3-5-exodeoxyribonuclease activity|
|regulation of mitotic recombination|
|meiotic DNA double-strand break formation|
|Mre11 complex|
|negative regulation of DNA endoreduplication|
|single-stranded DNA endodeoxyribonuclease activity|
|endodeoxyribonuclease activity|
|regulation of DNA endoreduplication|
|5-3 exonuclease activity|
|nuclease activity|
|intra-S DNA damage checkpoint|
|mitotic G2 DNA damage checkpoint|
|3-5 exonuclease activity|
|telomere capping|
|DNA strand elongation|
|telomere maintenance via telomerase|
|DNA double-strand break processing|
|negative regulation of DNA-dependent DNA replication|
|replication fork|
|positive regulation of protein autophosphorylation|
|mitotic G2/M transition checkpoint|
|telomere maintenance via telomere lengthening|
|G2 DNA damage checkpoint|
|replication fork processing|
|RNA-dependent DNA biosynthetic process|
|negative regulation of DNA replication|
|DNA-dependent DNA replication maintenance of fidelity|
|synapsis|
|regulation of protein autophosphorylation|
|sister chromatid cohesion|
|positive regulation of telomere maintenance|
|chromosome, telomeric region|
|reciprocal meiotic recombination|
|homologous recombination|
|regulation of DNA-dependent DNA replication|
|homologous chromosome segregation|
|double-strand break repair via nonhomologous end joining|
|site of double-strand break|
|DNA helicase activity|
|manganese ion binding|
|non-recombinational repair|
|chromosome organization involved in meiotic cell cycle|
|positive regulation of type I interferon production|
|regulation of telomere maintenance|
|meiotic chromosome segregation|
|negative regulation of G2/M transition of mitotic cell cycle|
|mitotic DNA damage checkpoint|
|double-strand break repair via homologous recombination|
|telomere maintenance|
|recombinational repair|
|PML body|
|negative regulation of cell cycle G2/M phase transition|
|telomere organization|
|double-stranded DNA binding|
|regulation of DNA recombination|
|mitotic DNA integrity checkpoint|
|regulation of DNA replication|
|meiosis I|
|nuclear chromosome, telomeric region|
|DNA biosynthetic process|
|DNA duplex unwinding|
|meiosis I cell cycle process|
|DNA geometric change|
|DNA-dependent DNA replication|
|regulation of type I interferon production|
|DNA damage checkpoint|
|sister chromatid segregation|
|DNA integrity checkpoint|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|meiotic cell cycle process|
|positive regulation of chromosome organization|
|regulation of signal transduction by p53 class mediator|
|double-strand break repair|
|protein C-terminus binding|
|positive regulation of DNA metabolic process|
|cell cycle checkpoint|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|negative regulation of mitotic cell cycle phase transition|
|DNA replication|
|nuclear chromosome segregation|
|DNA recombination|
|meiotic cell cycle|
|negative regulation of cell cycle phase transition|
|chromosome segregation|
|nuclear division|
|DNA conformation change|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|cadherin binding|
|organelle fission|
|negative regulation of cell cycle process|
|anatomical structure homeostasis|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|positive regulation of cytokine production|
|DNA repair|
|cell population proliferation|
|negative regulation of cell cycle|
|positive regulation of kinase activity|
|mitotic cell cycle process|
|positive regulation of organelle organization|
|regulation of mitotic cell cycle|
|positive regulation of transferase activity|
|mitotic cell cycle|
|regulation of cytokine production|
|viral process|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|symbiotic process|
|interspecies interaction between organisms|
|regulation of kinase activity|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|regulation of transferase activity|
|negative regulation of cell death|
|cell cycle process|
|positive regulation of protein phosphorylation|
|positive regulation of phosphorylation|
|chromosome organization|
|identical protein binding|
|nucleobase-containing compound biosynthetic process|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|regulation of organelle organization|
|organic cyclic compound biosynthetic process|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|regulation of programmed cell death|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|regulation of cell death|
|cellular response to stress|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-2.07|
|[[:results:exp222|Betulinic acid 10 to 15μM on day4 R05 exp222]]|-1.78|
|[[:results:exp533|TNF-alpha 44ng/ml R08 exp533]]|-1.74|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:pmvk|PMVK]]|0.423|
|[[:human genes:d:dnlz|DNLZ]]|0.417|
|[[:human genes:t:trmt61a|TRMT61A]]|0.408|
|[[:human genes:p:ppcdc|PPCDC]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 28/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|2/28|
|blood|0/28|
|bone|2/26|
|breast|1/33|
|central nervous system|4/56|
|cervix|1/4|
|colorectal|0/17|
|esophagus|2/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|1/21|
|liver|0/20|
|lung|2/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|1/22|
|urinary tract|2/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1822
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.92 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MRE11A Expression in NALM6 Cells: 6.92'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>