======= MTHFD2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MTHFD2
  * **<color #00a2e8>Official Name</color>**: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10797|10797]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P13995|P13995]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MTHFD2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MTHFD2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604887|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency. {ECO:0000269|PubMed:16100107}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|THF DHG CYH C|
|THF DHG CYH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|methenyltetrahydrofolate cyclohydrolase activity|
|methylenetetrahydrofolate dehydrogenase (NAD+) activity|
|methylenetetrahydrofolate dehydrogenase (NADP+) activity|
|tetrahydrofolate interconversion|
|phosphate ion binding|
|tetrahydrofolate metabolic process|
|folic acid metabolic process|
|one-carbon metabolic process|
|folic acid-containing compound metabolic process|
|pteridine-containing compound metabolic process|
|water-soluble vitamin metabolic process|
|dicarboxylic acid metabolic process|
|vitamin metabolic process|
|cellular modified amino acid metabolic process|
|magnesium ion binding|
|coenzyme metabolic process|
|mitochondrial matrix|
|cofactor metabolic process|
|drug metabolic process|
|cellular amide metabolic process|
|carboxylic acid metabolic process|
|oxidation-reduction process|
|oxoacid metabolic process|
|organic acid metabolic process|
|mitochondrion|
|extracellular space|
|small molecule metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|-2.77|
|[[:results:exp75|MK-1775 0.32μM R02 exp75]]|-2.45|
|[[:results:exp372|Ibrutinib 1μM R07 exp372]]|-1.71|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|1.7|
|[[:results:exp359|FK-506 30μM R07 exp359]]|1.71|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|1.71|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|1.77|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|1.81|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|1.83|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|1.85|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|1.88|
|[[:results:exp504|MK2206 4μM R08 exp504]]|1.9|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|1.92|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|1.94|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|1.94|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|1.95|
|[[:results:exp283|Glyphosate 1000μM R06 exp283]]|1.99|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|2.02|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|2.07|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|2.3|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|2.32|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|2.34|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|2.34|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|2.58|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|2.62|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|2.77|
|[[:results:exp274|Citral 50μM R06 exp274]]|2.97|
|[[:results:exp116|AICAR 240μM R03 exp116]]|5.41|
|[[:results:exp211|AICAR 240μM R05 exp211]]|5.45|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mthfd1l|MTHFD1L]]|0.661|
|[[:human genes:s:slc25a32|SLC25A32]]|0.658|
|[[:human genes:m:mtrr|MTRR]]|0.579|
|[[:human genes:s:shmt2|SHMT2]]|0.572|
|[[:human genes:m:mtr|MTR]]|0.518|
|[[:human genes:m:mmachc|MMACHC]]|0.455|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 4149
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.21 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MTHFD2 Expression in NALM6 Cells: 8.21'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>