======= NCBP2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NCBP2
  * **<color #00a2e8>Official Name</color>**: nuclear cap binding protein subunit 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=22916|22916]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P52298|P52298]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NCBP2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NCBP2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605133|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA- mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858). {ECO:0000269|PubMed:11551508, ECO:0000269|PubMed:15361857, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:17363367, ECO:0000269|PubMed:17873884, ECO:0000269|PubMed:18369367, ECO:0000269|PubMed:19632182, ECO:0000269|PubMed:26382858}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RRM 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|snRNA export from nucleus|
|RNA cap binding complex|
|nuclear cap binding complex|
|snRNA transport|
|snRNA binding|
|positive regulation of RNA export from nucleus|
|positive regulation of nucleobase-containing compound transport|
|positive regulation of mRNA 3-end processing|
|RNA cap binding|
|mRNA cap binding complex|
|pre-mRNA cleavage required for polyadenylation|
|mRNA cleavage involved in mRNA processing|
|RNA 7-methylguanosine cap binding|
|regulation of RNA export from nucleus|
|regulation of nucleobase-containing compound transport|
|regulation of mRNA polyadenylation|
|mRNA cleavage|
|regulation of mRNA 3-end processing|
|histone mRNA metabolic process|
|positive regulation of mRNA processing|
|mRNA cis splicing, via spliceosome|
|7-methylguanosine mRNA capping|
|termination of RNA polymerase II transcription|
|RNA capping|
|7-methylguanosine RNA capping|
|ncRNA export from nucleus|
|positive regulation of nucleocytoplasmic transport|
|snRNA transcription by RNA polymerase II|
|snRNA transcription|
|DNA-templated transcription, termination|
|transcription elongation from RNA polymerase II promoter|
|positive regulation of mRNA metabolic process|
|regulation of translational initiation|
|fibroblast growth factor receptor signaling pathway|
|mRNA 3-end processing|
|gene silencing by RNA|
|DNA-templated transcription, elongation|
|ncRNA transcription|
|regulation of nucleocytoplasmic transport|
|mRNA-containing ribonucleoprotein complex export from nucleus|
|mRNA export from nucleus|
|cellular response to fibroblast growth factor stimulus|
|response to fibroblast growth factor|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex localization|
|RNA export from nucleus|
|RNA 3-end processing|
|regulation of mRNA processing|
|protein export from nucleus|
|gene silencing|
|mRNA transport|
|nuclear export|
|RNA phosphodiester bond hydrolysis|
|mRNA binding|
|RNA transport|
|nucleic acid transport|
|establishment of RNA localization|
|nuclear-transcribed mRNA catabolic process|
|positive regulation of intracellular transport|
|RNA localization|
|mRNA catabolic process|
|nucleobase-containing compound transport|
|RNA catabolic process|
|protein-containing complex localization|
|nucleocytoplasmic transport|
|nuclear transport|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|nucleic acid phosphodiester bond hydrolysis|
|RNA splicing, via transesterification reactions|
|regulation of mRNA metabolic process|
|regulation of intracellular transport|
|regulation of translation|
|nucleobase-containing compound catabolic process|
|RNA splicing|
|regulation of cellular amide metabolic process|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|mRNA processing|
|organic cyclic compound catabolic process|
|transcription by RNA polymerase II|
|cellular response to growth factor stimulus|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|posttranscriptional regulation of gene expression|
|response to growth factor|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|mRNA metabolic process|
|enzyme linked receptor protein signaling pathway|
|RNA processing|
|cellular macromolecule catabolic process|
|regulation of cellular localization|
|positive regulation of transport|
|intracellular protein transport|
|macromolecule catabolic process|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|cellular response to endogenous stimulus|
|organic cyclic compound biosynthetic process|
|RNA binding|
|response to endogenous stimulus|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of transport|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-1.73|
|[[:results:exp274|Citral 50μM R06 exp274]]|1.89|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:ruvbl2|RUVBL2]]|0.49|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 726/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/25|
|breast|33/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|14/14|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 650
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.73 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NCBP2 Expression in NALM6 Cells: 6.73'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>