======= NELFE =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NELFE
  * **<color #00a2e8>Official Name</color>**: negative elongation factor complex member E
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7936|7936]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P18615|P18615]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NELFE&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NELFE|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/154040|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. {ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:11940650, ECO:0000269|PubMed:12612062, ECO:0000269|PubMed:18303858, ECO:0000269|PubMed:27282391, ECO:0000305}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RRM 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|NELF complex|
|negative regulation of mRNA polyadenylation|
|negative regulation of mRNA 3-end processing|
|negative regulation of transcription elongation from RNA polymerase II promoter|
|negative regulation of DNA-templated transcription, elongation|
|positive regulation of histone H3-K4 methylation|
|regulation of mRNA polyadenylation|
|regulation of histone H3-K4 methylation|
|negative regulation of mRNA processing|
|regulation of mRNA 3-end processing|
|regulation of transcription elongation from RNA polymerase II promoter|
|positive regulation of histone methylation|
|positive regulation of viral transcription|
|regulation of DNA-templated transcription, elongation|
|regulation of viral transcription|
|regulation of histone methylation|
|transcription elongation from RNA polymerase II promoter|
|negative regulation of mRNA metabolic process|
|positive regulation of histone modification|
|DNA-templated transcription, elongation|
|positive regulation of chromatin organization|
|positive regulation of viral process|
|regulation of mRNA processing|
|regulation of histone modification|
|positive regulation of chromosome organization|
|regulation of chromatin organization|
|regulation of viral process|
|positive regulation of ERK1 and ERK2 cascade|
|regulation of symbiosis, encompassing mutualism through parasitism|
|nuclear body|
|regulation of ERK1 and ERK2 cascade|
|regulation of mRNA metabolic process|
|regulation of chromosome organization|
|chromatin binding|
|transcription by RNA polymerase II|
|positive regulation of multi-organism process|
|positive regulation of MAPK cascade|
|positive regulation of organelle organization|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|regulation of MAPK cascade|
|regulation of multi-organism process|
|negative regulation of transcription by RNA polymerase II|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|positive regulation of phosphorylation|
|nucleobase-containing compound biosynthetic process|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|positive regulation of protein modification process|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|regulation of organelle organization|
|organic cyclic compound biosynthetic process|
|negative regulation of RNA metabolic process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|negative regulation of nucleobase-containing compound metabolic process|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of signal transduction|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp59|UMK57 1μM R01 exp59]]|-2.76|
|[[:results:exp46|HMS-I1 1μM R01 exp46]]|-2.5|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.24|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-2.19|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-2.05|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-2.03|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|-2|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|-1.93|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.88|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|-1.8|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|-1.73|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:n:nelfa|NELFA]]|0.487|
|[[:human genes:p:pds5b|PDS5B]]|0.413|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 9/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|1/28|
|blood|1/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|1/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|2/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2300
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NELFE Expression in NALM6 Cells: 5.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>