======= OGT =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: OGT
  * **<color #00a2e8>Official Name</color>**: O-linked N-acetylglucosamine (GlcNAc) transferase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=8473|8473]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O15294|O15294]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=OGT&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20OGT|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/300255|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc) (PubMed:26678539). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1 (PubMed:26678539). Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed:26678539). Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (PubMed:26678539). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6- phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:12150998, PubMed:18288188, PubMed:19377461, PubMed:19451179, PubMed:20018868, PubMed:20200153, PubMed:21285374, PubMed:15361863). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed:21285374, PubMed:28584052, PubMed:28302723). {ECO:0000269|PubMed:12150998, ECO:0000269|PubMed:15361863, ECO:0000269|PubMed:18288188, ECO:0000269|PubMed:19451179, ECO:0000269|PubMed:20018852, ECO:0000269|PubMed:20018868, ECO:0000269|PubMed:20200153, ECO:0000269|PubMed:21285374, ECO:0000269|PubMed:22121020, ECO:0000269|PubMed:22923583, ECO:0000269|PubMed:23353889, ECO:0000269|PubMed:24474760, ECO:0000269|PubMed:26678539, ECO:0000269|PubMed:28302723, ECO:0000269|PubMed:28584052}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|TPR 2|
|TPR 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|protein N-acetylglucosaminyltransferase complex|
|protein O-GlcNAc transferase activity|
|protein N-acetylglucosaminyltransferase activity|
|positive regulation of histone H3-K27 methylation|
|histone acetyltransferase activity (H4-K8 specific)|
|histone acetyltransferase activity (H4-K5 specific)|
|histone acetyltransferase activity (H4-K16 specific)|
|regulation of histone H3-K27 methylation|
|regulation of Rac protein signal transduction|
|histone H3-K4 trimethylation|
|histone H4-K8 acetylation|
|histone H4-K5 acetylation|
|histone H4-K16 acetylation|
|acetylglucosaminyltransferase activity|
|histone acetyltransferase complex|
|peptidyl-lysine trimethylation|
|negative regulation of proteasomal ubiquitin-dependent protein catabolic process|
|histone H3-K4 methylation|
|phosphatidylinositol-3,4,5-trisphosphate binding|
|positive regulation of histone methylation|
|regulation of gluconeogenesis|
|negative regulation of ubiquitin-dependent protein catabolic process|
|histone H4 acetylation|
|negative regulation of proteasomal protein catabolic process|
|circadian regulation of gene expression|
|regulation of insulin receptor signaling pathway|
|regulation of histone methylation|
|phosphatidylinositol-mediated signaling|
|negative regulation of proteolysis involved in cellular protein catabolic process|
|regulation of cellular response to insulin stimulus|
|histone lysine methylation|
|negative regulation of protein ubiquitination|
|inositol lipid-mediated signaling|
|regulation of glycolytic process|
|negative regulation of protein modification by small protein conjugation or removal|
|negative regulation of cellular protein catabolic process|
|regulation of carbohydrate catabolic process|
|peptidyl-lysine methylation|
|positive regulation of histone modification|
|histone methylation|
|regulation of carbohydrate biosynthetic process|
|positive regulation of cold-induced thermogenesis|
|cell projection|
|mitochondrial membrane|
|positive regulation of chromatin organization|
|protein O-linked glycosylation|
|histone acetylation|
|regulation of glucose metabolic process|
|regulation of purine nucleotide metabolic process|
|internal peptidyl-lysine acetylation|
|regulation of nucleotide metabolic process|
|regulation of ATP metabolic process|
|peptidyl-lysine acetylation|
|regulation of proteasomal ubiquitin-dependent protein catabolic process|
|internal protein amino acid acetylation|
|negative regulation of protein catabolic process|
|protein alkylation|
|protein methylation|
|regulation of cellular carbohydrate metabolic process|
|circadian rhythm|
|regulation of cold-induced thermogenesis|
|protein acetylation|
|regulation of histone modification|
|regulation of ubiquitin-dependent protein catabolic process|
|protein processing|
|regulation of generation of precursor metabolites and energy|
|positive regulation of chromosome organization|
|regulation of proteasomal protein catabolic process|
|protein acylation|
|regulation of chromatin organization|
|regulation of protein ubiquitination|
|regulation of carbohydrate metabolic process|
|response to nutrient|
|regulation of proteolysis involved in cellular protein catabolic process|
|protein maturation|
|response to insulin|
|regulation of protein modification by small protein conjugation or removal|
|regulation of Ras protein signal transduction|
|regulation of cellular protein catabolic process|
|macromolecule methylation|
|negative regulation of cellular catabolic process|
|protein glycosylation|
|macromolecule glycosylation|
|glycosylation|
|rhythmic process|
|protein deubiquitination|
|protein modification by small protein removal|
|negative regulation of catabolic process|
|methylation|
|peptidyl-lysine modification|
|glycoprotein biosynthetic process|
|regulation of small GTPase mediated signal transduction|
|regulation of chromosome organization|
|negative regulation of proteolysis|
|positive regulation of proteolysis|
|histone modification|
|covalent chromatin modification|
|regulation of protein catabolic process|
|glycoprotein metabolic process|
|response to peptide hormone|
|regulation of small molecule metabolic process|
|response to peptide|
|response to nutrient levels|
|response to extracellular stimulus|
|negative regulation of protein modification process|
|protein-containing complex|
|carbohydrate derivative biosynthetic process|
|positive regulation of organelle organization|
|chromatin organization|
|viral process|
|regulation of proteolysis|
|symbiotic process|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|peptidyl-amino acid modification|
|response to hormone|
|apoptotic process|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|response to organonitrogen compound|
|carbohydrate derivative metabolic process|
|negative regulation of cellular protein metabolic process|
|programmed cell death|
|chromosome organization|
|response to nitrogen compound|
|cell death|
|negative regulation of protein metabolic process|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|positive regulation of protein modification process|
|proteolysis|
|regulation of organelle organization|
|organonitrogen compound biosynthetic process|
|response to endogenous stimulus|
|positive regulation of transcription, DNA-templated|
|response to oxygen-containing compound|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp492|iCRT14 30μM R08 exp492]]|-2.36|
|[[:results:exp272|CHIR-124 0.04μM R06 exp272]]|-2.17|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|-1.84|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.73|
|[[:results:exp112|S-DABN 8μM R03 exp112]]|-1.72|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|1.71|
|[[:results:exp392|PT-1 25μM R07 exp392]]|1.74|
|[[:results:exp262|Alda-1 10μM R06 exp262]]|1.79|
|[[:results:exp101|Nicotinamide 1000μM R03 exp101]]|1.89|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|1.97|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|2|
|[[:results:exp504|MK2206 4μM R08 exp504]]|2.02|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hars|HARS]]|0.538|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 681/683
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|25/26|
|bone|25/25|
|breast|30/30|
|central nervous system|49/49|
|cervix|4/4|
|colorectal|17/17|
|esophagus|11/11|
|fibroblast|1/1|
|gastric|14/14|
|kidney|18/18|
|liver|18/19|
|lung|72/72|
|lymphocyte|14/14|
|ovary|25/25|
|pancreas|22/22|
|peripheral nervous system|15/15|
|plasma cell|12/12|
|prostate|1/1|
|skin|20/20|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|28/28|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 264
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.48 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='OGT Expression in NALM6 Cells: 9.48'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>