======= PDK3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PDK3
  * **<color #00a2e8>Official Name</color>**: pyruvate dehydrogenase kinase 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5165|5165]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q15120|Q15120]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PDK3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PDK3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/300906|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species. {ECO:0000269|PubMed:10748134, ECO:0000269|PubMed:11486000, ECO:0000269|PubMed:15861126, ECO:0000269|PubMed:16436377, ECO:0000269|PubMed:17683942, ECO:0000269|PubMed:18718909, ECO:0000269|PubMed:22865452}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|HATPase c|
|BCDHK Adom3|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|hypoxia-inducible factor-1alpha signaling pathway|
|pyruvate dehydrogenase (acetyl-transferring) kinase activity|
|peroxisome proliferator activated receptor signaling pathway|
|regulation of acetyl-CoA biosynthetic process from pyruvate|
|regulation of acyl-CoA biosynthetic process|
|regulation of coenzyme metabolic process|
|regulation of sulfur metabolic process|
|regulation of cofactor metabolic process|
|regulation of purine nucleotide biosynthetic process|
|regulation of nucleotide biosynthetic process|
|cellular response to fatty acid|
|cellular response to glucose stimulus|
|cellular response to hexose stimulus|
|cellular response to monosaccharide stimulus|
|cellular response to carbohydrate stimulus|
|response to fatty acid|
|regulation of fatty acid metabolic process|
|cellular glucose homeostasis|
|glucose metabolic process|
|regulation of glucose metabolic process|
|regulation of purine nucleotide metabolic process|
|regulation of nucleotide metabolic process|
|response to glucose|
|regulation of cellular carbohydrate metabolic process|
|response to hexose|
|response to monosaccharide|
|hexose metabolic process|
|intracellular receptor signaling pathway|
|response to carbohydrate|
|regulation of cellular ketone metabolic process|
|peptidyl-serine phosphorylation|
|regulation of reactive oxygen species metabolic process|
|glucose homeostasis|
|carbohydrate homeostasis|
|cellular response to hypoxia|
|cellular response to decreased oxygen levels|
|peptidyl-serine modification|
|monosaccharide metabolic process|
|regulation of carbohydrate metabolic process|
|cellular response to acid chemical|
|cellular response to oxygen levels|
|protein kinase activity|
|response to hypoxia|
|response to acid chemical|
|response to decreased oxygen levels|
|protein serine/threonine kinase activity|
|mitochondrial matrix|
|response to oxygen levels|
|regulation of lipid metabolic process|
|regulation of cellular amide metabolic process|
|regulation of small molecule metabolic process|
|carbohydrate metabolic process|
|cellular response to lipid|
|cellular chemical homeostasis|
|response to lipid|
|nucleolus|
|peptidyl-amino acid modification|
|cellular homeostasis|
|protein phosphorylation|
|cellular response to oxygen-containing compound|
|chemical homeostasis|
|response to abiotic stimulus|
|mitochondrion|
|phosphorylation|
|ATP binding|
|response to oxygen-containing compound|
|homeostatic process|
|cellular response to stress|
|small molecule metabolic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|-2.53|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|-2.32|
|[[:results:exp150|SGC0649 7μM R03 exp150]]|-2.14|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-2.01|
|[[:results:exp130|JQ1 0.01μM R03 exp130]]|-2|
|[[:results:exp139|Nicotinamide Riboside 100μM R03 exp139]]|-1.84|
|[[:results:exp112|S-DABN 8μM R03 exp112]]|-1.82|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|-1.7|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|1.74|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|1.84|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|2.65|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:a:aasdhppt|AASDHPPT]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/694
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/26|
|bone|0/26|
|breast|0/30|
|central nervous system|0/49|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/11|
|fibroblast|0/1|
|gastric|0/14|
|kidney|0/18|
|liver|0/19|
|lung|0/72|
|lymphocyte|0/16|
|ovary|0/25|
|pancreas|0/22|
|peripheral nervous system|0/15|
|plasma cell|0/12|
|prostate|0/1|
|skin|0/20|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/28|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 17040
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 3.5 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PDK3 Expression in NALM6 Cells: 3.5'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>