======= PDPK1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PDPK1
  * **<color #00a2e8>Official Name</color>**: 3-phosphoinositide dependent protein kinase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5170|5170]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O15530|O15530]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PDPK1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PDPK1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605213|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages. Isoform 3 is catalytically inactive. {ECO:0000269|PubMed:10226025, ECO:0000269|PubMed:10480933, ECO:0000269|PubMed:10995762, ECO:0000269|PubMed:12167717, ECO:0000269|PubMed:14585963, ECO:0000269|PubMed:14604990, ECO:0000269|PubMed:16207722, ECO:0000269|PubMed:16251192, ECO:0000269|PubMed:17327236, ECO:0000269|PubMed:17371830, ECO:0000269|PubMed:18835241, ECO:0000269|PubMed:9094314, ECO:0000269|PubMed:9445476, ECO:0000269|PubMed:9707564, ECO:0000269|PubMed:9768361}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|3-phosphoinositide-dependent protein kinase activity|
|phospholipase activator activity|
|cellular response to brain-derived neurotrophic factor stimulus|
|type B pancreatic cell development|
|positive regulation of vascular endothelial cell proliferation|
|phospholipase binding|
|regulation of vascular endothelial cell proliferation|
|type B pancreatic cell differentiation|
|glandular epithelial cell development|
|enteroendocrine cell differentiation|
|negative regulation of cardiac muscle cell apoptotic process|
|insulin receptor binding|
|hyperosmotic response|
|negative regulation of striated muscle cell apoptotic process|
|cell-substrate adherens junction assembly|
|focal adhesion assembly|
|negative regulation of endothelial cell apoptotic process|
|activation of protein kinase B activity|
|regulation of mast cell degranulation|
|regulation of mast cell activation involved in immune response|
|adherens junction assembly|
|regulation of cardiac muscle cell apoptotic process|
|negative regulation of muscle cell apoptotic process|
|endocrine pancreas development|
|glandular epithelial cell differentiation|
|positive regulation of sprouting angiogenesis|
|negative regulation of toll-like receptor signaling pathway|
|regulation of striated muscle cell apoptotic process|
|positive regulation of release of sequestered calcium ion into cytosol|
|negative regulation of epithelial cell apoptotic process|
|cell-substrate junction assembly|
|cellular response to epidermal growth factor stimulus|
|regulation of mast cell activation|
|response to epidermal growth factor|
|cellular response to nerve growth factor stimulus|
|columnar/cuboidal epithelial cell development|
|regulation of leukocyte degranulation|
|regulation of endothelial cell apoptotic process|
|epidermal growth factor receptor signaling pathway|
|response to nerve growth factor|
|positive regulation of calcium ion transport into cytosol|
|T cell costimulation|
|lymphocyte costimulation|
|positive regulation of blood vessel endothelial cell migration|
|positive regulation of protein localization to plasma membrane|
|regulation of myeloid leukocyte mediated immunity|
|positive regulation of phospholipase activity|
|positive regulation of protein localization to cell periphery|
|regulation of toll-like receptor signaling pathway|
|regulation of muscle cell apoptotic process|
|regulation of sprouting angiogenesis|
|ERBB signaling pathway|
|positive regulation of calcium ion transmembrane transport|
|regulation of phospholipase activity|
|positive regulation of lipase activity|
|pancreas development|
|negative regulation of transforming growth factor beta receptor signaling pathway|
|negative regulation of cellular response to transforming growth factor beta stimulus|
|response to osmotic stress|
|regulation of release of sequestered calcium ion into cytosol|
|regulation of epithelial cell apoptotic process|
|peptidyl-threonine phosphorylation|
|adherens junction organization|
|regulation of blood vessel endothelial cell migration|
|peptidyl-threonine modification|
|positive regulation of endothelial cell proliferation|
|regulation of protein localization to plasma membrane|
|columnar/cuboidal epithelial cell differentiation|
|extrinsic apoptotic signaling pathway|
|regulation of lipase activity|
|cell projection|
|regulation of calcium ion transport into cytosol|
|positive regulation of endothelial cell migration|
|stimulatory C-type lectin receptor signaling pathway|
|regulation of transforming growth factor beta receptor signaling pathway|
|regulation of protein localization to cell periphery|
|negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|innate immune response activating cell surface receptor signaling pathway|
|regulation of cellular response to transforming growth factor beta stimulus|
|positive regulation of calcium ion transport|
|endocrine system development|
|positive regulation of protein localization to membrane|
|regulation of sequestering of calcium ion|
|cell-matrix adhesion|
|regulation of endothelial cell proliferation|
|perikaryon|
|platelet activation|
|positive regulation of epithelial cell migration|
|positive regulation of cation transmembrane transport|
|negative regulation of neuron apoptotic process|
|calcium-mediated signaling|
|regulation of calcium ion transmembrane transport|
|negative regulation of cellular response to growth factor stimulus|
|positive regulation of ion transmembrane transport|
|regulation of endothelial cell migration|
|cellular response to insulin stimulus|
|positive regulation of angiogenesis|
|regulation of regulated secretory pathway|
|cell junction assembly|
|Fc-epsilon receptor signaling pathway|
|peptidyl-serine phosphorylation|
|T cell receptor signaling pathway|
|positive regulation of vasculature development|
|cell-substrate adhesion|
|regulation of protein localization to membrane|
|positive regulation of epithelial cell proliferation|
|epithelial cell development|
|protein autophosphorylation|
|peptidyl-serine modification|
|positive regulation of transmembrane transport|
|positive regulation of T cell activation|
|regulation of leukocyte mediated immunity|
|negative regulation of neuron death|
|regulation of neuron apoptotic process|
|cell junction organization|
|positive regulation of leukocyte cell-cell adhesion|
|positive regulation of immune effector process|
|regulation of epithelial cell migration|
|regulation of exocytosis|
|negative regulation of protein kinase activity|
|response to insulin|
|innate immune response-activating signal transduction|
|regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|Fc receptor signaling pathway|
|negative regulation of kinase activity|
|regulation of calcium ion transport|
|cytoplasmic vesicle|
|postsynaptic density|
|positive regulation of cell-cell adhesion|
|activation of innate immune response|
|cellular response to peptide hormone stimulus|
|regulation of cellular response to growth factor stimulus|
|positive regulation of ion transport|
|negative regulation of transferase activity|
|positive regulation of cytosolic calcium ion concentration|
|apoptotic signaling pathway|
|regulation of angiogenesis|
|blood coagulation|
|antigen receptor-mediated signaling pathway|
|coagulation|
|regulation of leukocyte cell-cell adhesion|
|hemostasis|
|regulation of neuron death|
|regulation of T cell activation|
|regulation of vasculature development|
|positive regulation of cellular protein localization|
|cellular response to peptide|
|regulation of cytosolic calcium ion concentration|
|activation of protein kinase activity|
|regulation of epithelial cell proliferation|
|regulation of cation transmembrane transport|
|second-messenger-mediated signaling|
|positive regulation of innate immune response|
|regulation of intracellular transport|
|positive regulation of lymphocyte activation|
|positive regulation of response to biotic stimulus|
|protein serine/threonine kinase activity|
|regulation of metal ion transport|
|response to peptide hormone|
|regulation of cell-cell adhesion|
|positive regulation of leukocyte activation|
|positive regulation of cell adhesion|
|negative regulation of protein phosphorylation|
|focal adhesion|
|positive regulation of cell activation|
|cellular calcium ion homeostasis|
|negative regulation of immune system process|
|negative regulation of phosphorylation|
|calcium ion homeostasis|
|immune response-activating cell surface receptor signaling pathway|
|regulation of innate immune response|
|protein kinase binding|
|regulation of immune effector process|
|cellular divalent inorganic cation homeostasis|
|response to peptide|
|positive regulation of defense response|
|regulation of ion transmembrane transport|
|wound healing|
|immune response-regulating cell surface receptor signaling pathway|
|divalent inorganic cation homeostasis|
|actin cytoskeleton organization|
|regulation of body fluid levels|
|positive regulation of cell migration|
|cellular response to growth factor stimulus|
|positive regulation of multi-organism process|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|regulation of lymphocyte activation|
|regulation of response to biotic stimulus|
|positive regulation of cell motility|
|response to growth factor|
|positive regulation of protein kinase activity|
|positive regulation of cellular component movement|
|regulation of cellular protein localization|
|regulation of vesicle-mediated transport|
|positive regulation of locomotion|
|cellular metal ion homeostasis|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|immune response-activating signal transduction|
|regulation of transmembrane transport|
|actin filament-based process|
|response to wounding|
|positive regulation of kinase activity|
|negative regulation of protein modification process|
|immune response-regulating signaling pathway|
|cellular response to organonitrogen compound|
|regulation of leukocyte activation|
|positive regulation of response to external stimulus|
|cellular response to hormone stimulus|
|metal ion homeostasis|
|activation of immune response|
|cellular cation homeostasis|
|regulation of cell activation|
|cellular ion homeostasis|
|positive regulation of transferase activity|
|cellular response to nitrogen compound|
|regulation of cell adhesion|
|epithelial cell differentiation|
|regulation of ion transport|
|cation homeostasis|
|enzyme linked receptor protein signaling pathway|
|inorganic ion homeostasis|
|cellular chemical homeostasis|
|regulation of secretion by cell|
|regulation of defense response|
|positive regulation of hydrolase activity|
|regulation of multi-organism process|
|ion homeostasis|
|negative regulation of catalytic activity|
|regulation of protein kinase activity|
|regulation of secretion|
|regulation of cell migration|
|positive regulation of immune response|
|regulation of kinase activity|
|peptidyl-amino acid modification|
|negative regulation of apoptotic process|
|cellular homeostasis|
|negative regulation of programmed cell death|
|response to hormone|
|regulation of cell motility|
|regulation of cellular localization|
|positive regulation of cell population proliferation|
|apoptotic process|
|cell adhesion|
|biological adhesion|
|cell migration|
|protein phosphorylation|
|regulation of transferase activity|
|regulation of locomotion|
|positive regulation of transport|
|regulation of cellular component movement|
|negative regulation of cell death|
|response to organonitrogen compound|
|positive regulation of protein phosphorylation|
|regulation of protein localization|
|negative regulation of cellular protein metabolic process|
|programmed cell death|
|cellular response to oxygen-containing compound|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|cell activation|
|cell motility|
|localization of cell|
|response to nitrogen compound|
|cell death|
|regulation of response to external stimulus|
|negative regulation of protein metabolic process|
|cytoskeleton organization|
|chemical homeostasis|
|epithelium development|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|negative regulation of molecular function|
|regulation of immune response|
|positive regulation of immune system process|
|response to abiotic stimulus|
|cellular response to endogenous stimulus|
|positive regulation of protein modification process|
|negative regulation of signal transduction|
|regulation of hydrolase activity|
|phosphorylation|
|locomotion|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of developmental process|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|response to endogenous stimulus|
|regulation of response to stress|
|ATP binding|
|regulation of apoptotic process|
|movement of cell or subcellular component|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|homeostatic process|
|cell development|
|regulation of immune system process|
|regulation of cell death|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|positive regulation of multicellular organismal process|
|tissue development|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|regulation of transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.71|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|1.81|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|1.81|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|1.86|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.08|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|2.1|
|[[:results:exp116|AICAR 240μM R03 exp116]]|2.11|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|2.16|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|2.17|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|2.19|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.35|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|2.4|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|2.66|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|2.74|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|3.13|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mapkap1|MAPKAP1]]|0.754|
|[[:human genes:r:rictor|RICTOR]]|0.71|
|[[:human genes:m:mlst8|MLST8]]|0.702|
|[[:human genes:a:akt2|AKT2]]|0.614|
|[[:human genes:c:cd81|CD81]]|0.607|
|[[:human genes:p:pik3cd|PIK3CD]]|0.566|
|[[:human genes:d:dohh|DOHH]]|0.546|
|[[:human genes:b:bak1|BAK1]]|0.538|
|[[:human genes:s:syk|SYK]]|0.524|
|[[:human genes:r:rhoh|RHOH]]|0.489|
|[[:human genes:r:romo1|ROMO1]]|0.486|
|[[:human genes:c:cd19|CD19]]|0.482|
|[[:human genes:m:mtor|MTOR]]|0.48|
|[[:human genes:g:gnas|GNAS]]|0.473|
|[[:human genes:a:akt1|AKT1]]|0.468|
|[[:human genes:v:vdac2|VDAC2]]|0.463|
|[[:human genes:r:rpn1|RPN1]]|0.46|
|[[:human genes:a:atp2a2|ATP2A2]]|0.456|
|[[:human genes:f:fam60a|FAM60A]]|0.446|
|[[:human genes:c:casp9|CASP9]]|0.445|
|[[:human genes:t:thg1l|THG1L]]|0.44|
|[[:human genes:t:taf4|TAF4]]|0.438|
|[[:human genes:r:rabif|RABIF]]|0.435|
|[[:human genes:s:srp14|SRP14]]|0.435|
|[[:human genes:a:apaf1|APAF1]]|0.433|
|[[:human genes:n:nsd1|NSD1]]|0.43|
|[[:human genes:b:bap1|BAP1]]|0.428|
|[[:human genes:p:plaa|PLAA]]|0.426|
|[[:human genes:t:trim28|TRIM28]]|0.426|
|[[:human genes:r:rptor|RPTOR]]|0.423|
|[[:human genes:h:hccs|HCCS]]|0.422|
|[[:human genes:u:usp42|USP42]]|0.421|
|[[:human genes:m:march5|MARCH5]]|0.421|
|[[:human genes:z:zzz3|ZZZ3]]|0.419|
|[[:human genes:f:faf2|FAF2]]|0.417|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.414|
|[[:human genes:u:utp23|UTP23]]|0.411|
|[[:human genes:a:alg13|ALG13]]|0.41|
|[[:human genes:t:taz|TAZ]]|0.409|
|[[:human genes:m:mis18a|MIS18A]]|0.407|
|[[:human genes:w:wdr82|WDR82]]|0.406|
|[[:human genes:p:pmm2|PMM2]]|0.405|
|[[:human genes:c:cbfb|CBFB]]|0.404|
|[[:human genes:h:hspd1|HSPD1]]|0.403|
|[[:human genes:l:lamtor4|LAMTOR4]]|0.403|
|[[:human genes:n:n6amt1|N6AMT1]]|0.403|
|[[:human genes:p:prrc2c|PRRC2C]]|0.402|
|[[:human genes:t:triap1|TRIAP1]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 15/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|2/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|1/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|2/24|
|peripheral nervous system|1/16|
|plasma cell|1/15|
|prostate|1/1|
|skin|1/24|
|soft tissue|1/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2391
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PDPK1 Expression in NALM6 Cells: 6.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>