======= PNP =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PNP
  * **<color #00a2e8>Official Name</color>**: purine nucleoside phosphorylase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4860|4860]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P00491|P00491]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PNP&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PNP|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/164050|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. {ECO:0000269|PubMed:2104852}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PNP UDP 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|purine-nucleoside phosphorylase activity|
|inosine catabolic process|
|pyridine nucleoside catabolic process|
|pyridine nucleoside metabolic process|
|nicotinamide riboside metabolic process|
|nicotinamide riboside catabolic process|
|purine nucleobase binding|
|interleukin-2 secretion|
|urate biosynthetic process|
|inosine metabolic process|
|nucleoside binding|
|pyridine-containing compound catabolic process|
|interleukin-2 production|
|purine ribonucleoside catabolic process|
|purine nucleoside catabolic process|
|phosphate ion binding|
|urate metabolic process|
|purine-containing compound salvage|
|ribonucleoside catabolic process|
|cellular metabolic compound salvage|
|nucleoside catabolic process|
|pyridine-containing compound metabolic process|
|glycosyl compound catabolic process|
|purine nucleotide catabolic process|
|positive regulation of alpha-beta T cell differentiation|
|cytokine secretion|
|nucleobase-containing small molecule catabolic process|
|purine-containing compound catabolic process|
|drug binding|
|positive regulation of alpha-beta T cell activation|
|purine ribonucleoside metabolic process|
|regulation of alpha-beta T cell differentiation|
|purine nucleoside metabolic process|
|nucleotide catabolic process|
|nucleoside phosphate catabolic process|
|ribonucleoside metabolic process|
|positive regulation of T cell differentiation|
|regulation of alpha-beta T cell activation|
|positive regulation of lymphocyte differentiation|
|positive regulation of T cell proliferation|
|nucleobase-containing small molecule biosynthetic process|
|nucleoside metabolic process|
|secretory granule lumen|
|ficolin-1-rich granule lumen|
|organophosphate catabolic process|
|positive regulation of lymphocyte proliferation|
|positive regulation of mononuclear cell proliferation|
|glycosyl compound metabolic process|
|positive regulation of leukocyte proliferation|
|regulation of T cell differentiation|
|positive regulation of leukocyte differentiation|
|cytokine production|
|regulation of T cell proliferation|
|protein secretion|
|establishment of protein localization to extracellular region|
|protein localization to extracellular region|
|regulation of lymphocyte differentiation|
|purine-containing compound biosynthetic process|
|peptide secretion|
|positive regulation of hemopoiesis|
|carbohydrate derivative catabolic process|
|positive regulation of T cell activation|
|regulation of lymphocyte proliferation|
|regulation of mononuclear cell proliferation|
|positive regulation of leukocyte cell-cell adhesion|
|regulation of leukocyte proliferation|
|nucleotide biosynthetic process|
|nucleoside phosphate biosynthetic process|
|positive regulation of cell-cell adhesion|
|regulation of leukocyte differentiation|
|regulation of leukocyte cell-cell adhesion|
|regulation of T cell activation|
|purine nucleotide metabolic process|
|positive regulation of lymphocyte activation|
|cytoskeleton|
|purine-containing compound metabolic process|
|nucleobase-containing compound catabolic process|
|regulation of cell-cell adhesion|
|positive regulation of leukocyte activation|
|positive regulation of cell adhesion|
|positive regulation of cell activation|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|small molecule catabolic process|
|aromatic compound catabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|regulation of hemopoiesis|
|organic cyclic compound catabolic process|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|neutrophil mediated immunity|
|neutrophil activation|
|granulocyte activation|
|leukocyte degranulation|
|regulation of lymphocyte activation|
|myeloid leukocyte mediated immunity|
|myeloid cell activation involved in immune response|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|myeloid leukocyte activation|
|small molecule biosynthetic process|
|regulation of leukocyte activation|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|regulation of cell activation|
|regulation of cell adhesion|
|regulated exocytosis|
|leukocyte mediated immunity|
|exocytosis|
|organophosphate metabolic process|
|positive regulation of cell population proliferation|
|leukocyte activation|
|positive regulation of cell differentiation|
|secretion by cell|
|response to drug|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|export from cell|
|organonitrogen compound catabolic process|
|cell activation|
|immune effector process|
|nucleobase-containing compound biosynthetic process|
|secretion|
|positive regulation of immune system process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|organic cyclic compound biosynthetic process|
|positive regulation of developmental process|
|organonitrogen compound biosynthetic process|
|protein transport|
|peptide transport|
|amide transport|
|establishment of protein localization|
|regulation of cell population proliferation|
|cellular nitrogen compound biosynthetic process|
|regulation of immune system process|
|positive regulation of multicellular organismal process|
|small molecule metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|regulation of cell differentiation|
|nitrogen compound transport|
|immune response|
|extracellular region|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|-2.24|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|-2.09|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|-2.07|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|-1.7|
|[[:results:exp359|FK-506 30μM R07 exp359]]|1.74|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|1.76|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|1.78|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|1.93|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|2.04|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 10246
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.4 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PNP Expression in NALM6 Cells: 7.4'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>