======= POLD3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLD3
  * **<color #00a2e8>Official Name</color>**: DNA polymerase delta 3, accessory subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10714|10714]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q15054|Q15054]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLD3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLD3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/611415|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906). {ECO:0000269|PubMed:10219083, ECO:0000269|PubMed:10852724, ECO:0000269|PubMed:11595739, ECO:0000269|PubMed:16510448, ECO:0000269|PubMed:19074196, ECO:0000269|PubMed:20227374, ECO:0000269|PubMed:20334433, ECO:0000269|PubMed:24035200, ECO:0000269|PubMed:24310611, ECO:0000269|PubMed:24449906, ECO:0000269|PubMed:25628356, ECO:0000269|PubMed:27185888}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CDC27|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|DNA synthesis involved in UV-damage excision repair|
|delta DNA polymerase complex|
|UV-damage excision repair|
|DNA strand elongation involved in DNA replication|
|DNA strand elongation|
|nucleotide-excision repair, DNA gap filling|
|telomere maintenance via semi-conservative replication|
|DNA-directed DNA polymerase activity|
|mismatch repair|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|DNA damage response, detection of DNA damage|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|nuclear DNA replication|
|cell cycle DNA replication|
|DNA synthesis involved in DNA repair|
|postreplication repair|
|transcription-coupled nucleotide-excision repair|
|cellular response to UV|
|telomere maintenance|
|telomere organization|
|DNA biosynthetic process|
|nucleotide-excision repair|
|cellular response to light stimulus|
|DNA-dependent DNA replication|
|response to UV|
|cellular response to radiation|
|DNA replication|
|nucleic acid phosphodiester bond hydrolysis|
|response to light stimulus|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|anatomical structure homeostasis|
|response to radiation|
|DNA repair|
|detection of stimulus|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular homeostasis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|organic cyclic compound biosynthetic process|
|cell cycle|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|-2.39|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|-2.36|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|-2.13|
|[[:results:exp532|TIC10 10μM R08 exp532]]|-2.12|
|[[:results:exp135|MS023 7μM R03 exp135]]|-2.06|
|[[:results:exp536|Vitamin-D3 40μM R08 exp536]]|-1.95|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-1.94|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|-1.92|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-1.91|
|[[:results:exp355|Dinaciclib 0.007μM R07 exp355]]|-1.86|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-1.81|
|[[:results:exp26|Oligomycin-A 20μM R00 exp26]]|-1.81|
|[[:results:exp465|Cannabidiol 13μM R08 exp465]]|-1.73|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-1.71|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-1.71|
|[[:results:exp415|Trichostatin-A 0.06μM R07 exp415]]|1.78|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|1.79|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|1.95|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|1.96|
|[[:results:exp124|GSK343 3μM R03 exp124]]|2.09|
|[[:results:exp156|UNC2400 2μM R03 exp156]]|2.14|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|2.2|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|2.22|
|[[:results:exp114|A-196 10μM R03 exp114]]|2.27|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|2.35|
|[[:results:exp426|FBS-Wisent 0.1 R07 exp426]]|2.46|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:cbwd5|CBWD5]]|0.414|
|[[:human genes:c:cbwd3|CBWD3]]|0.406|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 735/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|26/26|
|breast|33/33|
|central nervous system|56/56|
|cervix|3/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/16|
|kidney|20/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1380
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.3 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLD3 Expression in NALM6 Cells: 6.3'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>