======= POLR3A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLR3A
  * **<color #00a2e8>Official Name</color>**: RNA polymerase III subunit A
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=11128|11128]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O14802|O14802]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLR3A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLR3A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/614258|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. {ECO:0000250, ECO:0000269|PubMed:19609254, ECO:0000269|PubMed:19631370}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RNA pol Rpb1 3|
|RNA pol Rpb1 1|
|RNA pol Rpb1 5|
|RNA pol Rpb1 2|
|RNA pol Rpb1 4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|RNA polymerase III activity|
|RNA polymerase III complex|
|positive regulation of interferon-beta production|
|DNA-directed 5-3 RNA polymerase activity|
|transcription by RNA polymerase III|
|regulation of interferon-beta production|
|positive regulation of type I interferon production|
|regulation of type I interferon production|
|defense response to virus|
|response to virus|
|chromatin binding|
|positive regulation of cytokine production|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|regulation of cytokine production|
|innate immune response|
|defense response to other organism|
|immune effector process|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|response to other organism|
|organic cyclic compound biosynthetic process|
|response to external biotic stimulus|
|response to biotic stimulus|
|defense response|
|DNA binding|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|immune response|
|membrane|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-1.89|
|[[:results:exp225|Celastrol 0.12μM R05 exp225]]|-1.78|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|-1.75|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|-1.74|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|-1.7|
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|1.78|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|2.06|
|[[:results:exp4|Actinomycin-D 0.01μM R00 exp4]]|2.72|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:polr3b|POLR3B]]|0.425|
|[[:human genes:h:hars|HARS]]|0.424|
|[[:human genes:t:tbcd|TBCD]]|0.417|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 726/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/25|
|breast|33/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|14/14|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 714
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.67 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLR3A Expression in NALM6 Cells: 6.67'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>