======= RAD23B =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RAD23B
  * **<color #00a2e8>Official Name</color>**: RAD23 homolog B, nucleotide excision repair protein
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5887|5887]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P54727|P54727]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RAD23B&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RAD23B|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600062|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rad60-SLD|
|ubiquitin|
|UBA|
|XPC-binding|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|XPC complex|
|proteasome binding|
|nucleotide-excision repair, DNA duplex unwinding|
|nucleotide-excision repair, DNA damage recognition|
|global genome nucleotide-excision repair|
|polyubiquitin modification-dependent protein binding|
|nucleotide-excision repair, preincision complex assembly|
|cellular response to interleukin-7|
|response to interleukin-7|
|damaged DNA binding|
|proteasome complex|
|ubiquitin binding|
|single-stranded DNA binding|
|DNA duplex unwinding|
|nucleotide-excision repair|
|DNA geometric change|
|regulation of proteasomal ubiquitin-dependent protein catabolic process|
|regulation of ubiquitin-dependent protein catabolic process|
|regulation of proteasomal protein catabolic process|
|protein-DNA complex assembly|
|regulation of proteolysis involved in cellular protein catabolic process|
|protein folding|
|protein-DNA complex subunit organization|
|regulation of cellular protein catabolic process|
|protein deubiquitination|
|protein modification by small protein removal|
|DNA conformation change|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|proteasomal protein catabolic process|
|regulation of protein catabolic process|
|embryonic organ development|
|RNA polymerase II proximal promoter sequence-specific DNA binding|
|DNA repair|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|spermatogenesis|
|male gamete generation|
|proteolysis involved in cellular protein catabolic process|
|cellular protein catabolic process|
|protein catabolic process|
|gamete generation|
|regulation of proteolysis|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|multicellular organismal reproductive process|
|sexual reproduction|
|regulation of cellular catabolic process|
|cellular protein-containing complex assembly|
|multicellular organism reproduction|
|cellular macromolecule catabolic process|
|embryo development|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|multi-organism reproductive process|
|cellular response to cytokine stimulus|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|chromosome organization|
|response to cytokine|
|proteolysis|
|reproductive process|
|reproduction|
|protein-containing complex assembly|
|cellular response to stress|
|organic substance catabolic process|
|cellular catabolic process|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-2.54|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|1.75|
|[[:results:exp135|MS023 7μM R03 exp135]]|1.8|
|[[:results:exp53|Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53]]|1.82|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.87|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|1.87|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|1.91|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|1.91|
|[[:results:exp360|Genistein 15μM R07 exp360]]|1.95|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|2|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|2|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|2.03|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|2.09|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.09|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|2.15|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|2.24|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|2.35|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|2.99|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|3.06|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|3.07|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|3.65|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:plaa|PLAA]]|0.661|
|[[:human genes:c:casp9|CASP9]]|0.531|
|[[:human genes:d:dohh|DOHH]]|0.519|
|[[:human genes:a:apaf1|APAF1]]|0.505|
|[[:human genes:u:ubxn7|UBXN7]]|0.491|
|[[:human genes:b:bak1|BAK1]]|0.477|
|[[:human genes:u:ubqln1|UBQLN1]]|0.475|
|[[:human genes:u:ube2k|UBE2K]]|0.455|
|[[:human genes:v:vdac2|VDAC2]]|0.455|
|[[:human genes:z:zzz3|ZZZ3]]|0.452|
|[[:human genes:f:faf2|FAF2]]|0.435|
|[[:human genes:b:bap1|BAP1]]|0.434|
|[[:human genes:d:diablo|DIABLO]]|0.429|
|[[:human genes:h:hars|HARS]]|0.428|
|[[:human genes:t:thg1l|THG1L]]|0.423|
|[[:human genes:e:eny2|ENY2]]|0.423|
|[[:human genes:m:march5|MARCH5]]|0.421|
|[[:human genes:u:ube2g2|UBE2G2]]|0.411|
|[[:human genes:g:gabpa|GABPA]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 6455
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.29 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RAD23B Expression in NALM6 Cells: 7.29'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>