======= RBX1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RBX1
  * **<color #00a2e8>Official Name</color>**: ring-box 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9978|9978]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P62877|P62877]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RBX1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RBX1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603814|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription- coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. {ECO:0000269|PubMed:10579999, ECO:0000269|PubMed:11027288, ECO:0000269|PubMed:15983046, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:16751180, ECO:0000269|PubMed:18397884, ECO:0000269|PubMed:19112177, ECO:0000269|PubMed:19679664, ECO:0000269|PubMed:27565346}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|zf-rbx1|
|zf-Apc11|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|nuclear SCF ubiquitin ligase complex|
|NEDD8 transferase activity|
|Cul7-RING ubiquitin ligase complex|
|Cul4B-RING E3 ubiquitin ligase complex|
|VCB complex|
|SCF complex assembly|
|Cul5-RING ubiquitin ligase complex|
|cullin-RING ubiquitin ligase complex|
|Cul4A-RING E3 ubiquitin ligase complex|
|ubiquitin-ubiquitin ligase activity|
|protein neddylation|
|Cul2-RING ubiquitin ligase complex|
|cullin family protein binding|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|nucleotide-excision repair, DNA duplex unwinding|
|nucleotide-excision repair, DNA damage recognition|
|global genome nucleotide-excision repair|
|Cul4-RING E3 ubiquitin ligase complex|
|nucleotide-excision repair, preincision complex assembly|
|Cul3-RING ubiquitin ligase complex|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|DNA damage response, detection of DNA damage|
|nucleotide-excision repair, DNA incision|
|protein monoubiquitination|
|SCF ubiquitin ligase complex|
|transcription-coupled nucleotide-excision repair|
|regulation of transcription from RNA polymerase II promoter in response to hypoxia|
|positive regulation of proteasomal ubiquitin-dependent protein catabolic process|
|negative regulation of G2/M transition of mitotic cell cycle|
|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|
|interleukin-1-mediated signaling pathway|
|positive regulation of ubiquitin-dependent protein catabolic process|
|negative regulation of cell cycle G2/M phase transition|
|positive regulation of proteasomal protein catabolic process|
|nucleotide-excision repair|
|DNA duplex unwinding|
|DNA geometric change|
|regulation of transcription from RNA polymerase II promoter in response to stress|
|positive regulation of proteolysis involved in cellular protein catabolic process|
|regulation of proteasomal ubiquitin-dependent protein catabolic process|
|regulation of DNA-templated transcription in response to stress|
|positive regulation of cellular protein catabolic process|
|regulation of ubiquitin-dependent protein catabolic process|
|cellular response to interleukin-1|
|negative regulation of canonical Wnt signaling pathway|
|regulation of proteasomal protein catabolic process|
|cellular response to hypoxia|
|regulation of G2/M transition of mitotic cell cycle|
|cellular response to decreased oxygen levels|
|response to interleukin-1|
|protein-DNA complex assembly|
|negative regulation of Wnt signaling pathway|
|regulation of cell cycle G2/M phase transition|
|negative regulation of mitotic cell cycle phase transition|
|cellular response to oxygen levels|
|positive regulation of protein catabolic process|
|regulation of proteolysis involved in cellular protein catabolic process|
|ubiquitin protein ligase activity|
|negative regulation of cell cycle phase transition|
|protein-DNA complex subunit organization|
|ubiquitin-protein transferase activity|
|positive regulation of protein complex assembly|
|regulation of cellular protein catabolic process|
|protein-containing complex binding|
|regulation of canonical Wnt signaling pathway|
|ubiquitin protein ligase binding|
|DNA conformation change|
|protein polyubiquitination|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|negative regulation of cell cycle process|
|transcription factor binding|
|response to hypoxia|
|proteasomal protein catabolic process|
|Wnt signaling pathway|
|response to decreased oxygen levels|
|positive regulation of proteolysis|
|cell-cell signaling by wnt|
|regulation of Wnt signaling pathway|
|post-translational protein modification|
|positive regulation of cellular catabolic process|
|MAPK cascade|
|response to oxygen levels|
|regulation of protein catabolic process|
|signal transduction by protein phosphorylation|
|regulation of mitotic cell cycle phase transition|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|positive regulation of catabolic process|
|regulation of cell cycle phase transition|
|regulation of protein complex assembly|
|DNA repair|
|positive regulation of cellular component biogenesis|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|negative regulation of cell cycle|
|proteolysis involved in cellular protein catabolic process|
|cellular protein catabolic process|
|regulation of mitotic cell cycle|
|cytokine-mediated signaling pathway|
|protein catabolic process|
|protein ubiquitination|
|detection of stimulus|
|viral process|
|regulation of proteolysis|
|DNA metabolic process|
|regulation of cell cycle process|
|protein modification by small protein conjugation|
|cellular response to DNA damage stimulus|
|symbiotic process|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|zinc ion binding|
|cellular protein-containing complex assembly|
|cellular macromolecule catabolic process|
|regulation of cellular component biogenesis|
|protein phosphorylation|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|cellular response to cytokine stimulus|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|chromosome organization|
|response to cytokine|
|cell-cell signaling|
|response to abiotic stimulus|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|negative regulation of signal transduction|
|proteolysis|
|phosphorylation|
|negative regulation of cell communication|
|negative regulation of signaling|
|protein-containing complex assembly|
|positive regulation of cellular protein metabolic process|
|negative regulation of response to stimulus|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp41|BI-2536 0.001μM R01 exp41]]|-2.45|
|[[:results:exp61|YM155 0.0002μM R01 exp61]]|-2.1|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|-2.07|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|-1.97|
|[[:results:exp390|Pifithrin-alpha 20μM R07 exp390]]|-1.96|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|-1.88|
|[[:results:exp292|Menadione 5μM R06 exp292]]|1.79|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|2.14|
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|2.44|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 17/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|1/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|2/13|
|fibroblast|0/1|
|gastric|1/16|
|kidney|1/21|
|liver|0/20|
|lung|3/75|
|lymphocyte|1/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|1/9|
|thyroid|0/2|
|upper aerodigestive|1/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1025
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.11 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RBX1 Expression in NALM6 Cells: 5.11'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>