======= RFK =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RFK
  * **<color #00a2e8>Official Name</color>**: riboflavin kinase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55312|55312]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q969G6|Q969G6]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RFK&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RFK|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613010|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. {ECO:0000269|PubMed:19641494}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Flavokinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|FMN biosynthetic process|
|FMN metabolic process|
|riboflavin kinase activity|
|riboflavin biosynthetic process|
|flavin-containing compound biosynthetic process|
|positive regulation of NAD(P)H oxidase activity|
|riboflavin metabolic process|
|flavin-containing compound metabolic process|
|water-soluble vitamin biosynthetic process|
|regulation of NAD(P)H oxidase activity|
|vitamin biosynthetic process|
|ribonucleoside monophosphate biosynthetic process|
|nucleoside monophosphate biosynthetic process|
|ribonucleoside monophosphate metabolic process|
|positive regulation of oxidoreductase activity|
|nucleoside monophosphate metabolic process|
|water-soluble vitamin metabolic process|
|regulation of oxidoreductase activity|
|reactive oxygen species metabolic process|
|vitamin metabolic process|
|coenzyme biosynthetic process|
|ribonucleotide biosynthetic process|
|ribose phosphate biosynthetic process|
|cofactor biosynthetic process|
|nucleotide biosynthetic process|
|nucleoside phosphate biosynthetic process|
|coenzyme metabolic process|
|ribonucleotide metabolic process|
|ribose phosphate metabolic process|
|cofactor metabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|drug metabolic process|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|small molecule biosynthetic process|
|carbohydrate derivative biosynthetic process|
|organophosphate metabolic process|
|apoptotic process|
|carbohydrate derivative metabolic process|
|programmed cell death|
|cell death|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|organonitrogen compound biosynthetic process|
|positive regulation of catalytic activity|
|ATP binding|
|cellular nitrogen compound biosynthetic process|
|small molecule metabolic process|
|positive regulation of molecular function|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|-2.04|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|-1.81|
|[[:results:exp132|Metformin 40μM R03 exp132]]|-1.76|
|[[:results:exp463|Caffeine 2600μM R08 exp463]]|-1.73|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|1.7|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|1.72|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|1.74|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|1.81|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|1.82|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|1.87|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|1.88|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|1.88|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|1.89|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|1.93|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|1.94|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|1.95|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|2.31|
|[[:results:exp518|RK-33 8μM R08 exp518]]|2.36|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|2.41|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|2.43|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|2.67|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|2.93|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|3.5|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|6.24|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:s:slc25a19|SLC25A19]]|0.567|
|[[:human genes:t:thg1l|THG1L]]|0.561|
|[[:human genes:a:atxn10|ATXN10]]|0.545|
|[[:human genes:a:alg1|ALG1]]|0.54|
|[[:human genes:v:vezt|VEZT]]|0.53|
|[[:human genes:t:tpk1|TPK1]]|0.507|
|[[:human genes:a:atic|ATIC]]|0.495|
|[[:human genes:f:fh|FH]]|0.493|
|[[:human genes:g:gemin8|GEMIN8]]|0.493|
|[[:human genes:u:uhrf1|UHRF1]]|0.492|
|[[:human genes:r:rpp21|RPP21]]|0.486|
|[[:human genes:t:trmt5|TRMT5]]|0.483|
|[[:human genes:a:aasdhppt|AASDHPPT]]|0.48|
|[[:human genes:d:ddx1|DDX1]]|0.48|
|[[:human genes:d:ddx39a|DDX39A]]|0.471|
|[[:human genes:p:pyroxd1|PYROXD1]]|0.464|
|[[:human genes:g:gemin6|GEMIN6]]|0.464|
|[[:human genes:r:rpia|RPIA]]|0.462|
|[[:human genes:m:mrps33|MRPS33]]|0.458|
|[[:human genes:g:gclc|GCLC]]|0.455|
|[[:human genes:s:slc25a26|SLC25A26]]|0.455|
|[[:human genes:p:pmm2|PMM2]]|0.454|
|[[:human genes:a:adsl|ADSL]]|0.452|
|[[:human genes:d:dpagt1|DPAGT1]]|0.449|
|[[:human genes:t:ten1|TEN1]]|0.447|
|[[:human genes:c:c15orf41|C15orf41]]|0.446|
|[[:human genes:e:elp2|ELP2]]|0.441|
|[[:human genes:p:ppcdc|PPCDC]]|0.44|
|[[:human genes:t:ttc4|TTC4]]|0.439|
|[[:human genes:d:dr1|DR1]]|0.432|
|[[:human genes:t:trmt61a|TRMT61A]]|0.431|
|[[:human genes:z:zrsr2|ZRSR2]]|0.428|
|[[:human genes:e:elac2|ELAC2]]|0.428|
|[[:human genes:d:dolk|DOLK]]|0.427|
|[[:human genes:d:dld|DLD]]|0.427|
|[[:human genes:z:zcrb1|ZCRB1]]|0.427|
|[[:human genes:a:alg2|ALG2]]|0.426|
|[[:human genes:r:rabggtb|RABGGTB]]|0.426|
|[[:human genes:g:gtf3c6|GTF3C6]]|0.425|
|[[:human genes:p:ppcs|PPCS]]|0.424|
|[[:human genes:n:nbn|NBN]]|0.418|
|[[:human genes:r:rft1|RFT1]]|0.417|
|[[:human genes:c:ciao1|CIAO1]]|0.417|
|[[:human genes:m:mis18a|MIS18A]]|0.415|
|[[:human genes:h:hsd17b10|HSD17B10]]|0.413|
|[[:human genes:r:rtcb|RTCB]]|0.412|
|[[:human genes:t:triap1|TRIAP1]]|0.411|
|[[:human genes:r:rnmt|RNMT]]|0.411|
|[[:human genes:t:tsen54|TSEN54]]|0.411|
|[[:human genes:t:tbcb|TBCB]]|0.411|
|[[:human genes:t:tti1|TTI1]]|0.41|
|[[:human genes:d:dars2|DARS2]]|0.409|
|[[:human genes:u:uap1|UAP1]]|0.409|
|[[:human genes:s:srp14|SRP14]]|0.406|
|[[:human genes:t:tprkb|TPRKB]]|0.406|
|[[:human genes:g:gmppb|GMPPB]]|0.406|
|[[:human genes:u:uba3|UBA3]]|0.403|
|[[:human genes:t:timm10|TIMM10]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 41/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|2/28|
|blood|7/28|
|bone|0/26|
|breast|4/33|
|central nervous system|0/56|
|cervix|1/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|4/21|
|liver|0/20|
|lung|4/75|
|lymphocyte|3/16|
|ovary|2/26|
|pancreas|3/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 169
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.71 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RFK Expression in NALM6 Cells: 6.71'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>