======= RFWD2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: COP1
  * **<color #00a2e8>Official Name</color>**: COP1 E3 ubiquitin ligase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=64326|64326]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8NHY2|Q8NHY2]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RFWD2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RFWD2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608067|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). {ECO:0000269|PubMed:12466024, ECO:0000269|PubMed:12615916, ECO:0000269|PubMed:14739464, ECO:0000269|PubMed:15103385, ECO:0000269|PubMed:19805145, ECO:0000269|PubMed:19837670, ECO:0000269|PubMed:21625211, ECO:0000303|PubMed:27041596}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
|zf-C3HC4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Cul4A-RING E3 ubiquitin ligase complex|
|positive regulation of proteasomal ubiquitin-dependent protein catabolic process|
|positive regulation of ubiquitin-dependent protein catabolic process|
|positive regulation of proteasomal protein catabolic process|
|positive regulation of proteolysis involved in cellular protein catabolic process|
|regulation of proteasomal ubiquitin-dependent protein catabolic process|
|positive regulation of cellular protein catabolic process|
|response to ionizing radiation|
|regulation of ubiquitin-dependent protein catabolic process|
|regulation of proteasomal protein catabolic process|
|positive regulation of protein catabolic process|
|regulation of proteolysis involved in cellular protein catabolic process|
|ubiquitin protein ligase activity|
|ubiquitin-protein transferase activity|
|regulation of cellular protein catabolic process|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|proteasomal protein catabolic process|
|positive regulation of proteolysis|
|post-translational protein modification|
|positive regulation of cellular catabolic process|
|regulation of protein catabolic process|
|nuclear speck|
|positive regulation of catabolic process|
|response to radiation|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|proteolysis involved in cellular protein catabolic process|
|Golgi membrane|
|cellular protein catabolic process|
|protein catabolic process|
|protein ubiquitination|
|regulation of proteolysis|
|protein modification by small protein conjugation|
|regulation of cellular catabolic process|
|cellular macromolecule catabolic process|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|response to abiotic stimulus|
|proteolysis|
|positive regulation of cellular protein metabolic process|
|positive regulation of protein metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp480|ETC-159 50μM R08 exp480]]|-2.16|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|-1.8|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|1.72|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|1.73|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|1.76|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|1.77|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|1.77|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|1.8|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|1.8|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|1.81|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|1.84|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|1.88|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|1.94|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|2.25|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|2.8|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|3.11|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:d:dusp6|DUSP6]]|0.475|
|[[:human genes:d:det1|DET1]]|0.46|
|[[:human genes:l:lamtor4|LAMTOR4]]|0.455|
|[[:human genes:n:nf1|NF1]]|0.439|
|[[:human genes:s:spred2|SPRED2]]|0.425|
|[[:human genes:t:tsc22d2|TSC22D2]]|0.425|
|[[:human genes:t:tbc1d5|TBC1D5]]|0.422|
|[[:human genes:d:dgkd|DGKD]]|0.416|
|[[:human genes:l:lztr1|LZTR1]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 11/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|2/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5911
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.24 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RFWD2 Expression in NALM6 Cells: 5.24'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>