======= RHOH =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RHOH
  * **<color #00a2e8>Official Name</color>**: ras homolog family member H
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=399|399]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q15669|Q15669]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RHOH&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RHOH|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602037|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. {ECO:0000250, ECO:0000269|PubMed:11809807, ECO:0000269|PubMed:19414807}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Ras|
|Arf|
|Miro|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|kinase inhibitor activity|
|GTPase inhibitor activity|
|mast cell activation|
|Rho GTPase binding|
|immunological synapse|
|negative regulation of I-kappaB kinase/NF-kappaB signaling|
|negative regulation of GTPase activity|
|Rho protein signal transduction|
|cell projection|
|T cell differentiation|
|cell cortex|
|regulation of cell shape|
|establishment or maintenance of cell polarity|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|T cell activation|
|lymphocyte differentiation|
|actin filament organization|
|negative regulation of kinase activity|
|Ras protein signal transduction|
|negative regulation of transferase activity|
|small GTPase mediated signal transduction|
|GTPase activity|
|leukocyte differentiation|
|regulation of actin cytoskeleton organization|
|regulation of small GTPase mediated signal transduction|
|lymphocyte activation|
|regulation of actin filament-based process|
|GTP binding|
|negative regulation of phosphorylation|
|negative regulation of hydrolase activity|
|supramolecular fiber organization|
|protein kinase binding|
|regulation of GTPase activity|
|regulation of cell morphogenesis|
|actin cytoskeleton organization|
|negative regulation of intracellular signal transduction|
|regulation of cytoskeleton organization|
|hemopoiesis|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|actin filament-based process|
|myeloid leukocyte activation|
|hematopoietic or lymphoid organ development|
|immune system development|
|negative regulation of catalytic activity|
|regulation of kinase activity|
|leukocyte activation|
|regulation of transferase activity|
|regulation of anatomical structure morphogenesis|
|cell activation|
|cytoskeleton organization|
|negative regulation of molecular function|
|negative regulation of signal transduction|
|regulation of hydrolase activity|
|regulation of organelle organization|
|negative regulation of cell communication|
|negative regulation of signaling|
|regulation of phosphorylation|
|negative regulation of response to stimulus|
|intracellular signal transduction|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.6|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-2.3|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-1.83|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|-1.8|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-1.8|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-1.79|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-1.78|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|1.7|
|[[:results:exp224|CB-839 10μM R05 exp224]]|1.71|
|[[:results:exp519|RS-1 10μM R08 exp519]]|1.73|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|1.74|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.75|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|1.75|
|[[:results:exp458|Bisphenol S 100μM R08 exp458]]|1.76|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|1.77|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|1.77|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|1.78|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|1.8|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|1.82|
|[[:results:exp115|A-366 10μM R03 exp115]]|1.83|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|1.87|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|1.88|
|[[:results:exp275|Citral 75μM R06 exp275]]|1.89|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|1.96|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|1.99|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|1.99|
|[[:results:exp330|5-Azacytidine 2μM R07 exp330]]|2.02|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|2.08|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|2.19|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|2.24|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|2.25|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|2.34|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.51|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|2.53|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|2.55|
|[[:results:exp274|Citral 50μM R06 exp274]]|2.58|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|2.62|
|[[:results:exp453|B02 10μM R08 exp453]]|2.65|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|2.73|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.76|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|3.05|
|[[:results:exp116|AICAR 240μM R03 exp116]]|3.07|
|[[:results:exp134|MS023 2μM R03 exp134]]|3.1|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|3.24|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|3.33|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|3.33|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|3.41|
|[[:results:exp455|Benzoate 10000μM R08 exp455]]|3.56|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|4.02|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|4.15|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:pik3cd|PIK3CD]]|0.78|
|[[:human genes:c:cd19|CD19]]|0.762|
|[[:human genes:s:syk|SYK]]|0.755|
|[[:human genes:i:igll1|IGLL1]]|0.738|
|[[:human genes:c:cd81|CD81]]|0.655|
|[[:human genes:e:ergic1|ERGIC1]]|0.655|
|[[:human genes:c:cd79b|CD79B]]|0.653|
|[[:human genes:m:mapkap1|MAPKAP1]]|0.639|
|[[:human genes:c:cd79a|CD79A]]|0.629|
|[[:human genes:r:rictor|RICTOR]]|0.627|
|[[:human genes:a:akt2|AKT2]]|0.601|
|[[:human genes:v:vezf1|VEZF1]]|0.495|
|[[:human genes:p:pds5a|PDS5A]]|0.491|
|[[:human genes:p:pdpk1|PDPK1]]|0.489|
|[[:human genes:a:akt1|AKT1]]|0.487|
|[[:human genes:m:mlst8|MLST8]]|0.479|
|[[:human genes:s:ssr3|SSR3]]|0.446|
|[[:human genes:g:ganab|GANAB]]|0.443|
|[[:human genes:r:rbm41|RBM41]]|0.424|
|[[:human genes:p:pdcl|PDCL]]|0.42|
|[[:human genes:t:tox4|TOX4]]|0.409|
|[[:human genes:m:midn|MIDN]]|0.407|
|[[:human genes:s:ssr2|SSR2]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|1/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2334
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.32 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RHOH Expression in NALM6 Cells: 6.32'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>