======= RNF4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RNF4
  * **<color #00a2e8>Official Name</color>**: ring finger protein 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6047|6047]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P78317|P78317]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RNF4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RNF4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602850|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. {ECO:0000269|PubMed:12885770, ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:19307308, ECO:0000269|PubMed:20026589, ECO:0000269|PubMed:20212317, ECO:0000269|PubMed:20943951}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|zf-C3HC4|
|zf-rbx1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of kinetochore assembly|
|regulation of centromere complex assembly|
|SUMO polymer binding|
|protein K6-linked ubiquitination|
|nucleosome binding|
|regulation of spindle assembly|
|protein K11-linked ubiquitination|
|response to arsenic-containing substance|
|androgen receptor signaling pathway|
|protein K63-linked ubiquitination|
|regulation of spindle organization|
|androgen receptor binding|
|protein K48-linked ubiquitination|
|protein autoubiquitination|
|nuclear receptor transcription coactivator activity|
|intracellular steroid hormone receptor signaling pathway|
|PML body|
|steroid hormone mediated signaling pathway|
|intracellular receptor signaling pathway|
|hormone-mediated signaling pathway|
|cellular response to steroid hormone stimulus|
|regulation of microtubule cytoskeleton organization|
|regulation of organelle assembly|
|regulation of microtubule-based process|
|ubiquitin-protein transferase activity|
|transcription coactivator activity|
|protein polyubiquitination|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|response to steroid hormone|
|transcription factor binding|
|proteasomal protein catabolic process|
|regulation of chromosome organization|
|regulation of protein complex assembly|
|cellular response to lipid|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|regulation of cytoskeleton organization|
|cellular response to organic cyclic compound|
|modification-dependent macromolecule catabolic process|
|proteolysis involved in cellular protein catabolic process|
|cellular response to hormone stimulus|
|cellular protein catabolic process|
|protein catabolic process|
|protein ubiquitination|
|regulation of cell cycle process|
|protein modification by small protein conjugation|
|zinc ion binding|
|response to lipid|
|cellular macromolecule catabolic process|
|response to hormone|
|response to organic cyclic compound|
|regulation of cellular component biogenesis|
|protein modification by small protein conjugation or removal|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|identical protein binding|
|regulation of cell cycle|
|positive regulation of transcription by RNA polymerase II|
|cellular response to endogenous stimulus|
|proteolysis|
|regulation of organelle organization|
|DNA binding|
|response to endogenous stimulus|
|positive regulation of transcription, DNA-templated|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of RNA metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp485|GSK626616 14μM R08 exp485]]|-2.48|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-2.11|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-1.78|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-1.73|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|-1.7|
|[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|2.06|
|[[:results:exp100|NFN1 1μM R03 exp100]]|2.12|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.501|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.497|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.483|
|[[:human genes:z:zfand5|ZFAND5]]|0.482|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.471|
|[[:human genes:n:nup155|NUP155]]|0.468|
|[[:human genes:p:prpf8|PRPF8]]|0.456|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.445|
|[[:human genes:m:myo1h|MYO1H]]|0.443|
|[[:human genes:r:rps28|RPS28]]|0.443|
|[[:human genes:c:c14orf93|C14orf93]]|0.442|
|[[:human genes:s:stil|STIL]]|0.434|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.433|
|[[:human genes:s:scaf1|SCAF1]]|0.433|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.432|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.432|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.432|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.432|
|[[:human genes:z:znf106|ZNF106]]|0.428|
|[[:human genes:c:cope|COPE]]|0.427|
|[[:human genes:i:igfn1|IGFN1]]|0.424|
|[[:human genes:s:syt9|SYT9]]|0.422|
|[[:human genes:i:iscu|ISCU]]|0.421|
|[[:human genes:m:mdm2|MDM2]]|0.419|
|[[:human genes:t:twistnb|TWISTNB]]|0.419|
|[[:human genes:n:nupl1|NUPL1]]|0.415|
|[[:human genes:p:pola2|POLA2]]|0.415|
|[[:human genes:i:ints6|INTS6]]|0.414|
|[[:human genes:z:znf655|ZNF655]]|0.414|
|[[:human genes:r:rfxank|RFXANK]]|0.413|
|[[:human genes:r:rps2|RPS2]]|0.411|
|[[:human genes:c:copz1|COPZ1]]|0.408|
|[[:human genes:c:chd4|CHD4]]|0.406|
|[[:human genes:k:kif3c|KIF3C]]|0.405|
|[[:human genes:c:ccdc94|CCDC94]]|0.404|
|[[:human genes:o:orc1|ORC1]]|0.402|
|[[:human genes:v:vasn|VASN]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 148/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|8/28|
|blood|10/28|
|bone|9/25|
|breast|7/33|
|central nervous system|6/56|
|cervix|1/4|
|colorectal|2/17|
|esophagus|0/13|
|fibroblast|1/1|
|gastric|4/15|
|kidney|3/21|
|liver|6/20|
|lung|8/75|
|lymphocyte|3/14|
|ovary|9/26|
|pancreas|5/24|
|peripheral nervous system|2/16|
|plasma cell|3/15|
|prostate|0/1|
|skin|8/24|
|soft tissue|1/7|
|thyroid|1/2|
|upper aerodigestive|8/22|
|urinary tract|2/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 841
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.49 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RNF4 Expression in NALM6 Cells: 6.49'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>