======= ROBO1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ROBO1
  * **<color #00a2e8>Official Name</color>**: roundabout guidance receptor 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6091|6091]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9Y6N7|Q9Y6N7]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ROBO1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ROBO1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602430|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP- bound RHOA (PubMed:26529257). May be required for lung development (By similarity). {ECO:0000250|UniProtKB:O89026, ECO:0000269|PubMed:10102268, ECO:0000269|PubMed:24560577, ECO:0000269|PubMed:26529257, ECO:0000305}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|I-set|
|fn3|
|V-set|
|ig|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|chemorepulsion involved in postnatal olfactory bulb interneuron migration|
|negative regulation of negative chemotaxis|
|cerebral cortex tangential migration using cell-cell interactions|
|postnatal olfactory bulb interneuron migration|
|negative regulation of mammary gland epithelial cell proliferation|
|positive regulation of Notch signaling pathway involved in heart induction|
|regulation of Notch signaling pathway involved in heart induction|
|axon midline choice point recognition|
|regulation of negative chemotaxis|
|positive regulation of heart induction|
|Roundabout signaling pathway|
|substrate-dependent cerebral cortex tangential migration|
|LRR domain binding|
|positive regulation of vascular endothelial growth factor signaling pathway|
|axon choice point recognition|
|regulation of heart induction|
|negative regulation of chemokine-mediated signaling pathway|
|tangential migration from the subventricular zone to the olfactory bulb|
|olfactory bulb interneuron development|
|axon guidance receptor activity|
|cerebral cortex tangential migration|
|regulation of chemokine-mediated signaling pathway|
|olfactory bulb interneuron differentiation|
|regulation of mammary gland epithelial cell proliferation|
|regulation of vascular endothelial growth factor signaling pathway|
|pulmonary valve morphogenesis|
|regulation of cellular response to vascular endothelial growth factor stimulus|
|endocardial cushion formation|
|pulmonary valve development|
|cell migration involved in sprouting angiogenesis|
|substrate-dependent cell migration|
|outflow tract septum morphogenesis|
|aortic valve morphogenesis|
|positive regulation of Rho protein signal transduction|
|blood vessel endothelial cell migration|
|forebrain neuron development|
|olfactory bulb development|
|aortic valve development|
|olfactory lobe development|
|endocardial cushion morphogenesis|
|regulation of animal organ formation|
|semi-lunar valve development|
|regulation of heart morphogenesis|
|ventricular septum morphogenesis|
|cerebral cortex cell migration|
|endocardial cushion development|
|negative chemotaxis|
|neuron recognition|
|mesenchyme morphogenesis|
|aorta development|
|forebrain neuron differentiation|
|heart valve morphogenesis|
|positive regulation of Notch signaling pathway|
|negative regulation of chemotaxis|
|sprouting angiogenesis|
|telencephalon cell migration|
|heart valve development|
|forebrain cell migration|
|negative regulation of cytokine-mediated signaling pathway|
|positive regulation of Ras protein signal transduction|
|endothelial cell migration|
|negative regulation of response to cytokine stimulus|
|forebrain generation of neurons|
|endoplasmic reticulum-Golgi intermediate compartment membrane|
|positive regulation of small GTPase mediated signal transduction|
|ventricular septum development|
|outflow tract morphogenesis|
|cardiac septum morphogenesis|
|central nervous system neuron development|
|positive regulation of animal organ morphogenesis|
|epithelial cell migration|
|artery development|
|positive regulation of axonogenesis|
|epithelium migration|
|activation of cysteine-type endopeptidase activity involved in apoptotic process|
|tissue migration|
|regulation of Notch signaling pathway|
|cardiac septum development|
|cerebral cortex development|
|negative regulation of epithelial cell proliferation|
|cardiac chamber morphogenesis|
|cardiac ventricle development|
|positive regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|regulation of Rho protein signal transduction|
|positive regulation of cysteine-type endopeptidase activity|
|positive regulation of cell morphogenesis involved in differentiation|
|regulation of cytokine-mediated signaling pathway|
|homophilic cell adhesion via plasma membrane adhesion molecules|
|cardiac chamber development|
|ameboidal-type cell migration|
|positive regulation of endopeptidase activity|
|pallium development|
|regulation of response to cytokine stimulus|
|central nervous system neuron differentiation|
|regulation of axonogenesis|
|positive regulation of peptidase activity|
|regulation of chemotaxis|
|regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|mesenchyme development|
|cell recognition|
|regulation of cysteine-type endopeptidase activity|
|regulation of Ras protein signal transduction|
|heart morphogenesis|
|regulation of animal organ morphogenesis|
|telencephalon development|
|negative regulation of cell migration|
|cell-cell adhesion via plasma-membrane adhesion molecules|
|axon guidance|
|neuron projection guidance|
|positive regulation of MAP kinase activity|
|regulation of cellular response to growth factor stimulus|
|negative regulation of cell motility|
|positive regulation of neuron projection development|
|axon|
|regulation of cell morphogenesis involved in differentiation|
|negative regulation of cellular component movement|
|angiogenesis|
|negative regulation of locomotion|
|regulation of epithelial cell proliferation|
|positive regulation of protein serine/threonine kinase activity|
|regulation of MAP kinase activity|
|regulation of small GTPase mediated signal transduction|
|positive regulation of proteolysis|
|negative regulation of response to external stimulus|
|positive regulation of neuron differentiation|
|axonogenesis|
|positive regulation of cell projection organization|
|forebrain development|
|blood vessel morphogenesis|
|axon development|
|regulation of endopeptidase activity|
|cell morphogenesis involved in neuron differentiation|
|regulation of peptidase activity|
|positive regulation of neurogenesis|
|neuron projection morphogenesis|
|plasma membrane bounded cell projection morphogenesis|
|blood vessel development|
|cell projection morphogenesis|
|regulation of cell morphogenesis|
|regulation of neuron projection development|
|cell-cell adhesion|
|vasculature development|
|cell part morphogenesis|
|regulation of protein serine/threonine kinase activity|
|cardiovascular system development|
|heart development|
|positive regulation of protein kinase activity|
|positive regulation of nervous system development|
|positive regulation of cell development|
|positive regulation of MAPK cascade|
|chemotaxis|
|taxis|
|cell morphogenesis involved in differentiation|
|tissue morphogenesis|
|positive regulation of kinase activity|
|cell surface|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|tube morphogenesis|
|regulation of neuron differentiation|
|positive regulation of transferase activity|
|neuron projection development|
|negative regulation of cell population proliferation|
|regulation of plasma membrane bounded cell projection organization|
|positive regulation of cell death|
|regulation of cell projection organization|
|cell morphogenesis|
|regulation of proteolysis|
|brain development|
|regulation of MAPK cascade|
|positive regulation of hydrolase activity|
|head development|
|regulation of protein kinase activity|
|neuron development|
|regulation of neurogenesis|
|cellular component morphogenesis|
|tube development|
|regulation of cell migration|
|circulatory system development|
|regulation of kinase activity|
|anatomical structure formation involved in morphogenesis|
|regulation of cell motility|
|regulation of nervous system development|
|cell adhesion|
|regulation of cell development|
|biological adhesion|
|negative regulation of developmental process|
|animal organ morphogenesis|
|cell migration|
|positive regulation of cell differentiation|
|regulation of transferase activity|
|central nervous system development|
|regulation of locomotion|
|regulation of cellular component movement|
|neuron differentiation|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|identical protein binding|
|localization of cell|
|cell motility|
|regulation of response to external stimulus|
|plasma membrane bounded cell projection organization|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|cell projection organization|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|negative regulation of signal transduction|
|regulation of hydrolase activity|
|locomotion|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of developmental process|
|integral component of plasma membrane|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|generation of neurons|
|regulation of apoptotic process|
|movement of cell or subcellular component|
|regulation of programmed cell death|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|neurogenesis|
|cell development|
|positive regulation of signal transduction|
|regulation of cell death|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|positive regulation of multicellular organismal process|
|tissue development|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|-1.87|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-1.86|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-1.83|
|[[:results:exp471|Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471]]|-1.74|
|[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|1.73|
|[[:results:exp61|YM155 0.0002μM R01 exp61]]|1.93|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|2.01|
|[[:results:exp58|UM131593 0.1μM R01 exp58]]|2.06|
|[[:results:exp114|A-196 10μM R03 exp114]]|2.17|
|[[:results:exp534|Trientine 500μM R08 exp534]]|2.45|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.518|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5086
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 1.18 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ROBO1 Expression in NALM6 Cells: 1.18'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>