======= RPA3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPA3
  * **<color #00a2e8>Official Name</color>**: replication protein A3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6119|6119]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P35244|P35244]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPA3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPA3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/179837|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single- stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single- stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S- phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269|PubMed:19010961, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:19996105, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279, ECO:0000303|PubMed:24332808}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rep fac-A 3|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|DNA replication factor A complex|
|error-free translesion synthesis|
|error-prone translesion synthesis|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|nucleotide-excision repair, DNA gap filling|
|telomere maintenance via semi-conservative replication|
|nucleotide-excision repair, preincision complex assembly|
|mismatch repair|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|DNA damage response, detection of DNA damage|
|base-excision repair|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|nuclear DNA replication|
|cell cycle DNA replication|
|DNA synthesis involved in DNA repair|
|interstrand cross-link repair|
|postreplication repair|
|damaged DNA binding|
|site of double-strand break|
|transcription-coupled nucleotide-excision repair|
|regulation of cellular response to heat|
|double-strand break repair via homologous recombination|
|recombinational repair|
|telomere maintenance|
|telomere organization|
|single-stranded DNA binding|
|DNA biosynthetic process|
|nucleotide-excision repair|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|DNA-dependent DNA replication|
|double-strand break repair|
|regulation of signal transduction by p53 class mediator|
|protein-DNA complex assembly|
|DNA replication|
|DNA recombination|
|protein-DNA complex subunit organization|
|positive regulation of protein complex assembly|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nucleic acid phosphodiester bond hydrolysis|
|anatomical structure homeostasis|
|regulation of protein complex assembly|
|DNA repair|
|positive regulation of cellular component biogenesis|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|detection of stimulus|
|regulation of cellular response to stress|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|cellular homeostasis|
|regulation of cellular component biogenesis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|organic cyclic compound biosynthetic process|
|cell cycle|
|regulation of response to stress|
|protein-containing complex assembly|
|regulation of cell population proliferation|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-2.05|
|[[:results:exp40|2-Methoxyestradiol 0.2μM R01 exp40]]|2.24|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 667/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|26/28|
|blood|27/28|
|bone|25/26|
|breast|30/33|
|central nervous system|53/56|
|cervix|4/4|
|colorectal|14/17|
|esophagus|11/13|
|fibroblast|1/1|
|gastric|15/16|
|kidney|18/21|
|liver|19/20|
|lung|67/75|
|lymphocyte|13/16|
|ovary|25/26|
|pancreas|19/24|
|peripheral nervous system|15/16|
|plasma cell|14/15|
|prostate|1/1|
|skin|22/24|
|soft tissue|9/9|
|thyroid|1/2|
|upper aerodigestive|18/22|
|urinary tract|27/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1092
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.98 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPA3 Expression in NALM6 Cells: 4.98'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>