======= SART3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SART3
  * **<color #00a2e8>Official Name</color>**: spliceosome associated factor 3, U4/U6 recycling protein
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9733|9733]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q15020|Q15020]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SART3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SART3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/611684|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity). {ECO:0000250|UniProtKB:Q9JLI8, ECO:0000269|PubMed:12032085, ECO:0000269|PubMed:14749385, ECO:0000269|PubMed:20595234, ECO:0000269|PubMed:24526689}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RRM 1|
|Lsm interact|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|U6atac snRNA binding|
|U6atac snRNP|
|U4atac/U6atac snRNP|
|positive regulation of histone deubiquitination|
|regulation of histone deubiquitination|
|U4/U6 snRNP|
|ASAP complex|
|U4 snRNA binding|
|positive regulation of protein deubiquitination|
|U6 snRNA binding|
|spliceosomal tri-snRNP complex assembly|
|regulation of protein deubiquitination|
|ubiquitin-specific protease binding|
|U4/U6 x U5 tri-snRNP complex|
|spliceosomal snRNP assembly|
|Cajal body|
|regulation of alternative mRNA splicing, via spliceosome|
|positive regulation of histone modification|
|regulation of mRNA splicing, via spliceosome|
|positive regulation of chromatin organization|
|nucleosome assembly|
|histone binding|
|chromatin assembly|
|positive regulation of protein modification by small protein conjugation or removal|
|regulation of RNA splicing|
|regulation of mRNA processing|
|regulation of histone modification|
|chromatin assembly or disassembly|
|nucleosome organization|
|positive regulation of chromosome organization|
|DNA packaging|
|regulation of chromatin organization|
|protein-DNA complex assembly|
|regulation of protein modification by small protein conjugation or removal|
|ribonucleoprotein complex assembly|
|protein-DNA complex subunit organization|
|ribonucleoprotein complex subunit organization|
|mRNA splicing, via spliceosome|
|DNA conformation change|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|RNA splicing, via transesterification reactions|
|regulation of mRNA metabolic process|
|regulation of chromosome organization|
|positive regulation of proteolysis|
|RNA splicing|
|nuclear speck|
|ribonucleoprotein complex biogenesis|
|mRNA processing|
|positive regulation of organelle organization|
|mRNA metabolic process|
|chromatin organization|
|regulation of proteolysis|
|cellular protein-containing complex assembly|
|RNA processing|
|chromosome organization|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|regulation of organelle organization|
|RNA binding|
|protein-containing complex assembly|
|positive regulation of cellular protein metabolic process|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|regulation of protein modification process|
|protein-containing complex subunit organization|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-2.79|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|-2.64|
|[[:results:exp61|YM155 0.0002μM R01 exp61]]|-2.62|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|-2.47|
|[[:results:exp279|D-Fructose 10000μM R06 exp279]]|-2.35|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|-2.26|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|-2.05|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-1.95|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|-1.95|
|[[:results:exp262|Alda-1 10μM R06 exp262]]|-1.9|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-1.89|
|[[:results:exp285|GW501516 25μM R06 exp285]]|-1.87|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-1.84|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|-1.83|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|-1.83|
|[[:results:exp291|LLY-284 2.6μM R06 exp291]]|-1.75|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|-1.72|
|[[:results:exp516|Pyrazinamide 100μM R08 exp516]]|-1.71|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|1.76|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|1.84|
|[[:results:exp114|A-196 10μM R03 exp114]]|2.09|
|[[:results:exp239|PFI-2 4μM R05 exp239]]|2.7|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|3.52|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.479|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 516/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|19/28|
|blood|16/28|
|bone|17/26|
|breast|27/33|
|central nervous system|43/56|
|cervix|2/4|
|colorectal|12/17|
|esophagus|7/13|
|fibroblast|1/1|
|gastric|12/16|
|kidney|11/21|
|liver|14/20|
|lung|47/75|
|lymphocyte|12/16|
|ovary|23/26|
|pancreas|17/24|
|peripheral nervous system|8/16|
|plasma cell|8/15|
|prostate|1/1|
|skin|17/24|
|soft tissue|7/9|
|thyroid|1/2|
|upper aerodigestive|15/22|
|urinary tract|17/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2078
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.17 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SART3 Expression in NALM6 Cells: 7.17'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>