======= SFPQ =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SFPQ
  * **<color #00a2e8>Official Name</color>**: splicing factor proline and glutamine rich
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6421|6421]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P23246|P23246]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SFPQ&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SFPQ|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605199|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA (PubMed:25765647). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Functions as transcriptional activator (PubMed:25765647). Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation (By similarity). Required for the assembly of nuclear speckles (PubMed:25765647). Plays a role in the regulation of DNA virus- mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). {ECO:0000250|UniProtKB:Q8VIJ6, ECO:0000269|PubMed:10847580, ECO:0000269|PubMed:10858305, ECO:0000269|PubMed:10931916, ECO:0000269|PubMed:11259580, ECO:0000269|PubMed:11525732, ECO:0000269|PubMed:11897684, ECO:0000269|PubMed:15590677, ECO:0000269|PubMed:20932480, ECO:0000269|PubMed:25765647, ECO:0000269|PubMed:28712728, ECO:0000269|PubMed:8045264, ECO:0000269|PubMed:8449401}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|NOPS|
|RRM 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|dendritic transport of ribonucleoprotein complex|
|dendritic transport of messenger ribonucleoprotein complex|
|positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway|
|paraspeckles|
|dendritic transport|
|positive regulation of sister chromatid cohesion|
|axo-dendritic protein transport|
|positive regulation of oxidative stress-induced cell death|
|negative regulation of circadian rhythm|
|positive regulation of cellular response to oxidative stress|
|alternative mRNA splicing, via spliceosome|
|positive regulation of response to oxidative stress|
|histone H3 deacetylation|
|regulation of sister chromatid cohesion|
|regulation of oxidative stress-induced intrinsic apoptotic signaling pathway|
|positive regulation of chromosome segregation|
|dendrite cytoplasm|
|histone deacetylation|
|E-box binding|
|protein deacetylation|
|positive regulation of intrinsic apoptotic signaling pathway|
|protein deacylation|
|macromolecule deacylation|
|regulation of oxidative stress-induced cell death|
|axo-dendritic transport|
|microtubule-based protein transport|
|protein transport along microtubule|
|RNA polymerase II transcription factor complex|
|regulation of cellular response to oxidative stress|
|transcription regulatory region sequence-specific DNA binding|
|regulation of sister chromatid segregation|
|regulation of response to oxidative stress|
|double-strand break repair via homologous recombination|
|RNA polymerase II distal enhancer sequence-specific DNA binding|
|recombinational repair|
|regulation of chromosome segregation|
|nuclear matrix|
|histone deacetylase binding|
|chromatin|
|regulation of circadian rhythm|
|transport along microtubule|
|regulation of intrinsic apoptotic signaling pathway|
|positive regulation of apoptotic signaling pathway|
|positive regulation of chromosome organization|
|cytoskeleton-dependent intracellular transport|
|double-strand break repair|
|microtubule-based transport|
|RNA transport|
|nucleic acid transport|
|establishment of RNA localization|
|RNA localization|
|transcription regulatory region DNA binding|
|DNA recombination|
|nucleobase-containing compound transport|
|protein-containing complex localization|
|activation of innate immune response|
|microtubule-based movement|
|rhythmic process|
|positive regulation of cell cycle process|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions|
|positive regulation of innate immune response|
|regulation of chromosome organization|
|positive regulation of response to biotic stimulus|
|histone modification|
|covalent chromatin modification|
|positive regulation of cell cycle|
|chromatin binding|
|regulation of apoptotic signaling pathway|
|RNA splicing|
|nuclear speck|
|regulation of innate immune response|
|positive regulation of defense response|
|mRNA processing|
|DNA repair|
|positive regulation of multi-organism process|
|regulation of response to biotic stimulus|
|positive regulation of response to external stimulus|
|positive regulation of organelle organization|
|activation of immune response|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|microtubule-based process|
|mRNA metabolic process|
|positive regulation of cell death|
|chromatin organization|
|regulation of cellular response to stress|
|DNA metabolic process|
|regulation of cell cycle process|
|regulation of defense response|
|innate immune response|
|cellular response to DNA damage stimulus|
|regulation of multi-organism process|
|negative regulation of transcription by RNA polymerase II|
|positive regulation of immune response|
|RNA processing|
|protein homodimerization activity|
|defense response to other organism|
|intracellular protein transport|
|positive regulation of intracellular signal transduction|
|chromosome organization|
|regulation of response to external stimulus|
|regulation of immune response|
|positive regulation of immune system process|
|regulation of cell cycle|
|negative regulation of transcription, DNA-templated|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|regulation of organelle organization|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|defense response|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|regulation of response to stress|
|protein transport|
|negative regulation of cellular biosynthetic process|
|intracellular transport|
|peptide transport|
|regulation of apoptotic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|movement of cell or subcellular component|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|regulation of programmed cell death|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of signal transduction|
|regulation of immune system process|
|RNA metabolic process|
|regulation of cell death|
|cellular response to stress|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|nitrogen compound transport|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|1.7|
|[[:results:exp235|Geldanamycin 0.01μM R05 exp235]]|1.71|
|[[:results:exp130|JQ1 0.01μM R03 exp130]]|1.73|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|1.76|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|1.77|
|[[:results:exp279|D-Fructose 10000μM R06 exp279]]|1.81|
|[[:results:exp295|Pyronaridine 1μM R06 exp295]]|1.84|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|1.89|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|1.9|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|2.06|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|2.16|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:v:vasn|VASN]]|0.524|
|[[:human genes:r:rrm1|RRM1]]|0.494|
|[[:human genes:p:pan2|PAN2]]|0.492|
|[[:human genes:s:supt6h|SUPT6H]]|0.491|
|[[:human genes:p:psmd1|PSMD1]]|0.482|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.481|
|[[:human genes:p:pan3|PAN3]]|0.474|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.459|
|[[:human genes:p:psmd11|PSMD11]]|0.456|
|[[:human genes:h:hist2h3a|HIST2H3A]]|0.451|
|[[:human genes:h:hist2h3c|HIST2H3C]]|0.45|
|[[:human genes:p:psmc2|PSMC2]]|0.449|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.448|
|[[:human genes:s:scaf1|SCAF1]]|0.446|
|[[:human genes:c:cope|COPE]]|0.443|
|[[:human genes:d:dync1i2|DYNC1I2]]|0.44|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.44|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.439|
|[[:human genes:c:chd4|CHD4]]|0.439|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.439|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.439|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.439|
|[[:human genes:p:pcf11|PCF11]]|0.435|
|[[:human genes:p:psmd7|PSMD7]]|0.434|
|[[:human genes:t:tceb3cl|TCEB3CL]]|0.434|
|[[:human genes:f:fbxo5|FBXO5]]|0.433|
|[[:human genes:c:cdca3|CDCA3]]|0.43|
|[[:human genes:r:rfxank|RFXANK]]|0.428|
|[[:human genes:t:tceb3c|TCEB3C]]|0.427|
|[[:human genes:n:nop16|NOP16]]|0.427|
|[[:human genes:a:atxn1|ATXN1]]|0.426|
|[[:human genes:c:c14orf93|C14orf93]]|0.425|
|[[:human genes:r:rps12|RPS12]]|0.425|
|[[:human genes:c:cbwd5|CBWD5]]|0.423|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.421|
|[[:human genes:a:aqr|AQR]]|0.42|
|[[:human genes:r:rps9|RPS9]]|0.42|
|[[:human genes:s:s1pr4|S1PR4]]|0.42|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.419|
|[[:human genes:n:nol12|NOL12]]|0.417|
|[[:human genes:t:ticrr|TICRR]]|0.415|
|[[:human genes:s:ssh2|SSH2]]|0.415|
|[[:human genes:b:brd4|BRD4]]|0.415|
|[[:human genes:k:krt36|KRT36]]|0.415|
|[[:human genes:r:rad21|RAD21]]|0.415|
|[[:human genes:t:terf2|TERF2]]|0.415|
|[[:human genes:d:ddtl|DDTL]]|0.414|
|[[:human genes:r:rps2|RPS2]]|0.413|
|[[:human genes:z:znf106|ZNF106]]|0.411|
|[[:human genes:c:cbwd3|CBWD3]]|0.407|
|[[:human genes:m:med14|MED14]]|0.407|
|[[:human genes:i:igfn1|IGFN1]]|0.405|
|[[:human genes:r:rbbp5|RBBP5]]|0.403|
|[[:human genes:n:npipb5|NPIPB5]]|0.403|
|[[:human genes:p:pola2|POLA2]]|0.402|
|[[:human genes:d:dtl|DTL]]|0.401|
|[[:human genes:h:hist1h3i|HIST1H3I]]|0.401|
|[[:human genes:m:mt1e|MT1E]]|0.401|
|[[:human genes:p:psmc1|PSMC1]]|0.4|
|[[:human genes:p:psmd3|PSMD3]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 721/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|27/28|
|bone|25/25|
|breast|32/33|
|central nervous system|54/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|14/14|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 717
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SFPQ Expression in NALM6 Cells: 9.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>