======= SLX4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SLX4
  * **<color #00a2e8>Official Name</color>**: SLX4 structure-specific endonuclease subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=84464|84464]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8IY92|Q8IY92]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SLX4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SLX4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613278|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein that functions as an assembly component of multiple structure-specific endonucleases. These endonuclease complexes are required for repair of specific types of DNA lesions and critical for cellular responses to replication fork failure. Mutations in this gene were found in patients with Fanconi anemia. [provided by RefSeq, Sep 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure- specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81- EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. {ECO:0000269|PubMed:19595721, ECO:0000269|PubMed:19595722, ECO:0000269|PubMed:19596235, ECO:0000269|PubMed:19596236}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|BTB|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Slx1-Slx4 complex|
|3-flap endonuclease activity|
|positive regulation of t-circle formation|
|ERCC4-ERCC1 complex|
|meiotic DNA double-strand break processing|
|response to intra-S DNA damage checkpoint signaling|
|Holliday junction resolvase complex|
|DNA double-strand break processing involved in repair via single-strand annealing|
|double-strand break repair via single-strand annealing|
|response to DNA damage checkpoint signaling|
|response to cell cycle checkpoint signaling|
|5-flap endonuclease activity|
|response to DNA integrity checkpoint signaling|
|telomeric D-loop disassembly|
|regulation of t-circle formation|
|crossover junction endodeoxyribonuclease activity|
|t-circle formation|
|telomere maintenance via telomere trimming|
|formation of extrachromosomal circular DNA|
|telomeric loop disassembly|
|resolution of meiotic recombination intermediates|
|meiotic chromosome separation|
|DNA double-strand break processing|
|negative regulation of telomere maintenance via telomere lengthening|
|chromosome separation|
|negative regulation of telomere maintenance|
|enzyme activator activity|
|positive regulation of telomere maintenance|
|chromosome, telomeric region|
|reciprocal meiotic recombination|
|interstrand cross-link repair|
|homologous recombination|
|regulation of telomere maintenance via telomere lengthening|
|non-recombinational repair|
|regulation of telomere maintenance|
|meiotic chromosome segregation|
|double-strand break repair via homologous recombination|
|recombinational repair|
|telomere maintenance|
|telomere organization|
|meiosis I|
|nuclear chromosome, telomeric region|
|nucleotide-excision repair|
|meiosis I cell cycle process|
|negative regulation of DNA metabolic process|
|negative regulation of chromosome organization|
|meiotic nuclear division|
|meiotic cell cycle process|
|positive regulation of chromosome organization|
|double-strand break repair|
|positive regulation of DNA metabolic process|
|DNA replication|
|cellular response to biotic stimulus|
|nuclear chromosome segregation|
|DNA recombination|
|meiotic cell cycle|
|nuclear chromatin|
|chromosome segregation|
|nuclear division|
|organelle fission|
|anatomical structure homeostasis|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|negative regulation of organelle organization|
|DNA repair|
|positive regulation of cellular component biogenesis|
|cell junction|
|positive regulation of organelle organization|
|negative regulation of cellular component organization|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|regulation of cellular component biogenesis|
|cell cycle process|
|chromosome organization|
|positive regulation of cellular component organization|
|cellular response to endogenous stimulus|
|regulation of organelle organization|
|response to biotic stimulus|
|cell cycle|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|negative regulation of nucleobase-containing compound metabolic process|
|response to endogenous stimulus|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|positive regulation of nucleobase-containing compound metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|-1.94|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|-1.91|
|[[:results:exp146|Quinacrine 2.5μM R03 exp146]]|-1.85|
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|-1.7|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2712
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.71 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SLX4 Expression in NALM6 Cells: 5.71'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>