======= SPAG5 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SPAG5
  * **<color #00a2e8>Official Name</color>**: sperm associated antigen 5
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10615|10615]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96R06|Q96R06]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SPAG5&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SPAG5|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/615562|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457). {ECO:0000269|PubMed:12356910, ECO:0000269|PubMed:17664331, ECO:0000269|PubMed:18055457, ECO:0000269|PubMed:18361916, ECO:0000269|PubMed:21402792, ECO:0000269|PubMed:23953116, ECO:0000269|PubMed:26297806, ECO:0000269|PubMed:27462074, ECO:0000305|PubMed:11724960}.

<button type='default' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
No Pfam Domain information is available for this gene.
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of spindle assembly|
|regulation of metaphase plate congression|
|regulation of attachment of spindle microtubules to kinetochore|
|microtubule plus-end|
|protein localization to microtubule organizing center|
|protein localization to centrosome|
|mitotic spindle pole|
|regulation of spindle assembly|
|centriolar satellite|
|protein localization to microtubule cytoskeleton|
|protein localization to cytoskeleton|
|regulation of spindle organization|
|positive regulation of organelle assembly|
|mitotic spindle|
|kinetochore|
|condensed chromosome kinetochore|
|regulation of chromosome segregation|
|mitotic sister chromatid segregation|
|sister chromatid segregation|
|mitotic nuclear division|
|spindle organization|
|midbody|
|regulation of microtubule cytoskeleton organization|
|positive regulation of intracellular transport|
|nuclear chromosome segregation|
|regulation of organelle assembly|
|positive regulation of cytoskeleton organization|
|regulation of microtubule-based process|
|microtubule binding|
|chromosome segregation|
|nuclear division|
|positive regulation of cell cycle process|
|organelle fission|
|regulation of intracellular transport|
|positive regulation of cell cycle|
|microtubule cytoskeleton organization|
|cell division|
|positive regulation of cellular component biogenesis|
|regulation of cytoskeleton organization|
|mitotic cell cycle process|
|positive regulation of organelle organization|
|microtubule-based process|
|mitotic cell cycle|
|protein localization to organelle|
|regulation of cell cycle process|
|regulation of cellular localization|
|regulation of cellular component biogenesis|
|positive regulation of transport|
|cell cycle process|
|chromosome organization|
|cytoskeleton organization|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|regulation of organelle organization|
|cell cycle|
|cellular protein localization|
|cellular macromolecule localization|
|regulation of transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-3.13|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|-2.24|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-2.14|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-2.06|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-2.01|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|-1.91|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-1.84|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|-1.72|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|-1.71|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-1.71|
|[[:results:exp40|2-Methoxyestradiol 0.2μM R01 exp40]]|2.01|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|2.05|
|[[:results:exp61|YM155 0.0002μM R01 exp61]]|2.16|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|2.54|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:u:ube2c|UBE2C]]|0.417|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2983
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.82 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SPAG5 Expression in NALM6 Cells: 7.82'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>