======= TOP2A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TOP2A
  * **<color #00a2e8>Official Name</color>**: DNA topoisomerase II alpha
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7153|7153]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P11388|P11388]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TOP2A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TOP2A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/126430|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DNA gyraseB|
|HATPase c|
|DTHCT|
|DNA topoisoIV|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|DNA topoisomerase complex (ATP-hydrolyzing)|
|viral integration complex|
|DNA topoisomerase type II (ATP-hydrolyzing) activity|
|positive regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|negative regulation of DNA duplex unwinding|
|apoptotic chromosome condensation|
|regulation of DNA duplex unwinding|
|embryonic cleavage|
|DNA topological change|
|DNA ligation|
|regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|resolution of meiotic recombination intermediates|
|DNA binding, bending|
|meiotic chromosome separation|
|apoptotic nuclear changes|
|cellular component disassembly involved in execution phase of apoptosis|
|condensed chromosome|
|nuclear chromosome|
|chromosome separation|
|female meiotic nuclear division|
|positive regulation of viral genome replication|
|chromosome condensation|
|DNA-dependent ATPase activity|
|reciprocal meiotic recombination|
|homologous recombination|
|protein kinase C binding|
|execution phase of apoptosis|
|drug binding|
|positive regulation of viral life cycle|
|ubiquitin binding|
|hematopoietic progenitor cell differentiation|
|meiotic chromosome segregation|
|regulation of viral genome replication|
|mitotic DNA integrity checkpoint|
|positive regulation of viral process|
|meiosis I|
|histone deacetylase binding|
|meiosis I cell cycle process|
|regulation of circadian rhythm|
|female gamete generation|
|negative regulation of chromosome organization|
|centriole|
|sister chromatid segregation|
|DNA integrity checkpoint|
|regulation of viral life cycle|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|ribonucleoprotein complex|
|meiotic cell cycle process|
|DNA packaging|
|protein C-terminus binding|
|cell cycle checkpoint|
|regulation of viral process|
|magnesium ion binding|
|nuclear chromosome segregation|
|regulation of symbiosis, encompassing mutualism through parasitism|
|DNA recombination|
|meiotic cell cycle|
|chromosome segregation|
|rhythmic process|
|nuclear division|
|DNA conformation change|
|negative regulation of mitotic cell cycle|
|organelle fission|
|enzyme binding|
|regulation of chromosome organization|
|cellular process involved in reproduction in multicellular organism|
|negative regulation of organelle organization|
|chromatin binding|
|cellular component disassembly|
|protein heterodimerization activity|
|cell division|
|positive regulation of multi-organism process|
|hemopoiesis|
|negative regulation of cell cycle|
|protein-containing complex|
|mitotic cell cycle process|
|hematopoietic or lymphoid organ development|
|regulation of mitotic cell cycle|
|positive regulation of apoptotic process|
|immune system development|
|positive regulation of programmed cell death|
|mitotic cell cycle|
|gamete generation|
|positive regulation of cell death|
|negative regulation of cellular component organization|
|DNA metabolic process|
|regulation of multi-organism process|
|cellular response to DNA damage stimulus|
|multicellular organismal reproductive process|
|sexual reproduction|
|multicellular organism reproduction|
|nucleolus|
|protein homodimerization activity|
|apoptotic process|
|embryo development|
|multi-organism reproductive process|
|cell cycle process|
|programmed cell death|
|chromosome organization|
|cell death|
|regulation of cell cycle|
|positive regulation of transcription by RNA polymerase II|
|regulation of organelle organization|
|cell cycle|
|RNA binding|
|reproductive process|
|reproduction|
|DNA binding|
|ATP binding|
|positive regulation of transcription, DNA-templated|
|regulation of apoptotic process|
|regulation of programmed cell death|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of cell death|
|cellular response to stress|
|positive regulation of RNA metabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-1.94|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|-1.76|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|1.72|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|1.78|
|[[:results:exp391|Pomalidomide 20μM R07 exp391]]|1.87|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|1.91|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|2.09|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|2.16|
|[[:results:exp59|UMK57 1μM R01 exp59]]|2.29|
|[[:results:exp210|LB-100 2μM R05 exp210]]|2.47|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|3.39|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|4.11|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|6.09|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|8.37|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|8.37|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.497|
|[[:human genes:t:top2b|TOP2B]]|0.412|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 727/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|24/28|
|bone|25/26|
|breast|31/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|15/16|
|plasma cell|15/15|
|prostate|0/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1059
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TOP2A Expression in NALM6 Cells: 9.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>