======= TOP2B =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TOP2B
  * **<color #00a2e8>Official Name</color>**: DNA topoisomerase II beta
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7155|7155]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q02880|Q02880]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TOP2B&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TOP2B|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/126431|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. {ECO:0000269|PubMed:10684600}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DNA topoisoIV|
|DTHCT|
|DNA gyraseB|
|HATPase c|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|viral integration complex|
|positive regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|DNA topoisomerase type II (ATP-hydrolyzing) activity|
|DNA topological change|
|regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|resolution of meiotic recombination intermediates|
|meiotic chromosome separation|
|heterochromatin|
|chromosome separation|
|positive regulation of viral genome replication|
|reciprocal meiotic recombination|
|homologous recombination|
|protein kinase C binding|
|positive regulation of viral life cycle|
|meiotic chromosome segregation|
|regulation of viral genome replication|
|mitotic DNA integrity checkpoint|
|positive regulation of viral process|
|meiosis I|
|histone deacetylase binding|
|meiosis I cell cycle process|
|neuron migration|
|sister chromatid segregation|
|DNA integrity checkpoint|
|regulation of viral life cycle|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|ribonucleoprotein complex|
|meiotic cell cycle process|
|protein C-terminus binding|
|cell cycle checkpoint|
|regulation of viral process|
|nuclear chromosome segregation|
|regulation of symbiosis, encompassing mutualism through parasitism|
|DNA recombination|
|meiotic cell cycle|
|chromosome segregation|
|nuclear division|
|DNA conformation change|
|negative regulation of mitotic cell cycle|
|organelle fission|
|enzyme binding|
|axonogenesis|
|forebrain development|
|chromatin binding|
|axon development|
|cell morphogenesis involved in neuron differentiation|
|neuron projection morphogenesis|
|plasma membrane bounded cell projection morphogenesis|
|protein heterodimerization activity|
|cell projection morphogenesis|
|positive regulation of multi-organism process|
|cell part morphogenesis|
|cell morphogenesis involved in differentiation|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|neuron projection development|
|mitotic cell cycle|
|cell morphogenesis|
|brain development|
|DNA metabolic process|
|regulation of multi-organism process|
|head development|
|neuron development|
|cellular component morphogenesis|
|nucleolus|
|cell migration|
|central nervous system development|
|cell cycle process|
|neuron differentiation|
|chromosome organization|
|cell motility|
|localization of cell|
|plasma membrane bounded cell projection organization|
|cell projection organization|
|regulation of cell cycle|
|locomotion|
|cell cycle|
|reproductive process|
|reproduction|
|DNA binding|
|ATP binding|
|generation of neurons|
|movement of cell or subcellular component|
|neurogenesis|
|cell development|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp19|Etoposide 1μM R00 exp19]]|1.71|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|1.91|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|3.28|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|4.37|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|4.79|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|7.21|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|7.74|
|[[:results:exp360|Genistein 15μM R07 exp360]]|8.37|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hars|HARS]]|0.438|
|[[:human genes:e:eri1|ERI1]]|0.413|
|[[:human genes:t:top2a|TOP2A]]|0.412|
|[[:human genes:u:ubxn7|UBXN7]]|0.405|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 14091
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 10.42 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TOP2B Expression in NALM6 Cells: 10.42'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>