======= VPRBP =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DCAF1
  * **<color #00a2e8>Official Name</color>**: DDB1 and CUL4 associated factor 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9730|9730]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9Y4B6|Q9Y4B6]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=VPRBP&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20VPRBP|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/617259|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1- DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine- protein kinase that specifically mediates phosphorylation of 'Thr- 120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714). {ECO:0000269|PubMed:16964240, ECO:0000269|PubMed:17609381, ECO:0000269|PubMed:17630831, ECO:0000269|PubMed:18332868, ECO:0000269|PubMed:18524771, ECO:0000269|PubMed:18606781, ECO:0000269|PubMed:19287380, ECO:0000269|PubMed:20644714, ECO:0000269|PubMed:22184063, ECO:0000269|PubMed:23063525, ECO:0000269|PubMed:23362280, ECO:0000269|PubMed:24140421}. (Microbial infection) In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A- RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. {ECO:0000269|PubMed:17314515, ECO:0000269|PubMed:18464893, ECO:0000269|PubMed:19264781, ECO:0000269|PubMed:19923175, ECO:0000269|PubMed:24336198}.

<button type='default' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
No Pfam Domain information is available for this gene.
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|histone kinase activity (H2A-T120 specific)|
|cell competition in a multicellular organism|
|histone H2A-T120 phosphorylation|
|histone H2A phosphorylation|
|histone-threonine phosphorylation|
|V(D)J recombination|
|Cul4-RING E3 ubiquitin ligase complex|
|histone phosphorylation|
|somatic cell DNA recombination|
|COP9 signalosome|
|somatic diversification of immune receptors via germline recombination within a single locus|
|estrogen receptor binding|
|somatic diversification of immune receptors|
|peptidyl-threonine phosphorylation|
|peptidyl-threonine modification|
|B cell differentiation|
|fibrillar center|
|B cell activation|
|DNA recombination|
|lymphocyte differentiation|
|leukocyte differentiation|
|histone modification|
|covalent chromatin modification|
|lymphocyte activation|
|hemopoiesis|
|hematopoietic or lymphoid organ development|
|immune system development|
|regulation of growth|
|protein ubiquitination|
|chromatin organization|
|viral process|
|DNA metabolic process|
|protein modification by small protein conjugation|
|symbiotic process|
|interspecies interaction between organisms|
|negative regulation of transcription by RNA polymerase II|
|peptidyl-amino acid modification|
|leukocyte activation|
|protein phosphorylation|
|protein modification by small protein conjugation or removal|
|chromosome organization|
|cell activation|
|negative regulation of transcription, DNA-templated|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|phosphorylation|
|negative regulation of RNA metabolic process|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|ATP binding|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|negative regulation of gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-1.89|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-1.81|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|1.72|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|1.81|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|1.85|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|1.88|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|2.01|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|2.1|
|[[:results:exp114|A-196 10μM R03 exp114]]|2.15|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|2.15|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.458|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: N/A
^Tissue^Fraction Of Cell Lines Where Essential^
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 548
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.1 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='VPRBP Expression in NALM6 Cells: 7.1'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>