======= WAS =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: WAS
  * **<color #00a2e8>Official Name</color>**: WASP actin nucleation promoting factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7454|7454]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P42768|P42768]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=WAS&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20WAS|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/300392|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:   The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton.  The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins.  Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients.  A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. {ECO:0000269|PubMed:12235133, ECO:0000269|PubMed:16275905, ECO:0000269|PubMed:18650809}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PBD|
|WH2|
|WH1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of T cell antigen processing and presentation|
|Cdc42 protein signal transduction|
|actin cortical patch organization|
|actin cortical patch assembly|
|actin cortical patch localization|
|positive regulation of Arp2/3 complex-mediated actin nucleation|
|positive regulation of double-strand break repair via homologous recombination|
|GTPase regulator activity|
|positive regulation of actin nucleation|
|phospholipase binding|
|regulation of Arp2/3 complex-mediated actin nucleation|
|actin cortical patch|
|regulation of antigen processing and presentation|
|negative regulation of stress fiber assembly|
|small GTPase binding|
|regulation of double-strand break repair via nonhomologous end joining|
|negative regulation of actin filament bundle assembly|
|regulation of actin nucleation|
|vesicle membrane|
|regulation of lamellipodium assembly|
|positive regulation of double-strand break repair|
|actin filament polymerization|
|positive regulation of DNA recombination|
|regulation of lamellipodium organization|
|cortical actin cytoskeleton organization|
|regulation of double-strand break repair via homologous recombination|
|cortical cytoskeleton organization|
|actin polymerization or depolymerization|
|Rac GTPase binding|
|site of double-strand break|
|phagocytic vesicle|
|positive regulation of DNA repair|
|regulation of T cell mediated immunity|
|regulation of double-strand break repair|
|Rho protein signal transduction|
|regulation of stress fiber assembly|
|actin filament|
|protein polymerization|
|regulation of actomyosin structure organization|
|positive regulation of actin filament polymerization|
|regulation of actin filament bundle assembly|
|positive regulation of response to DNA damage stimulus|
|regulation of DNA recombination|
|actin filament-based movement|
|regulation of DNA repair|
|positive regulation of protein polymerization|
|negative regulation of supramolecular fiber organization|
|SH3 domain binding|
|Fc-gamma receptor signaling pathway involved in phagocytosis|
|immune response-regulating cell surface receptor signaling pathway involved in phagocytosis|
|Fc receptor mediated stimulatory signaling pathway|
|negative regulation of cytoskeleton organization|
|Fc-gamma receptor signaling pathway|
|regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains|
|regulation of lymphocyte mediated immunity|
|regulation of adaptive immune response|
|cellular response to interferon-gamma|
|regulation of actin filament polymerization|
|cell-cell junction|
|T cell receptor signaling pathway|
|response to interferon-gamma|
|regulation of actin polymerization or depolymerization|
|regulation of actin filament length|
|positive regulation of DNA metabolic process|
|positive regulation of supramolecular fiber organization|
|regulation of plasma membrane bounded cell projection assembly|
|regulation of cell projection assembly|
|actin filament binding|
|regulation of leukocyte mediated immunity|
|actin cytoskeleton|
|regulation of response to DNA damage stimulus|
|positive regulation of cytoskeleton organization|
|regulation of protein polymerization|
|endosomal transport|
|T cell activation|
|actin filament organization|
|Fc receptor signaling pathway|
|Ras protein signal transduction|
|positive regulation of protein complex assembly|
|regulation of actin filament organization|
|negative regulation of cell motility|
|blood coagulation|
|coagulation|
|antigen receptor-mediated signaling pathway|
|hemostasis|
|negative regulation of cellular component movement|
|small GTPase mediated signal transduction|
|negative regulation of locomotion|
|phagocytosis|
|regulation of actin cytoskeleton organization|
|regulation of supramolecular fiber organization|
|regulation of DNA metabolic process|
|negative regulation of organelle organization|
|regulation of cellular component size|
|lymphocyte activation|
|regulation of actin filament-based process|
|epidermis development|
|immune response-activating cell surface receptor signaling pathway|
|regulation of protein complex assembly|
|supramolecular fiber organization|
|protein kinase binding|
|regulation of immune effector process|
|wound healing|
|immune response-regulating cell surface receptor signaling pathway|
|actin cytoskeleton organization|
|regulation of body fluid levels|
|regulation of anatomical structure size|
|positive regulation of cellular component biogenesis|
|regulation of cytoskeleton organization|
|endocytosis|
|immune response-activating signal transduction|
|actin filament-based process|
|response to wounding|
|immune response-regulating signaling pathway|
|positive regulation of organelle organization|
|activation of immune response|
|import into cell|
|regulation of plasma membrane bounded cell projection organization|
|negative regulation of cellular component organization|
|regulation of cell projection organization|
|regulation of cellular response to stress|
|innate immune response|
|cellular protein-containing complex assembly|
|positive regulation of immune response|
|regulation of cell motility|
|leukocyte activation|
|defense response to other organism|
|regulation of cellular component biogenesis|
|regulation of locomotion|
|regulation of cellular component movement|
|cellular response to cytokine stimulus|
|identical protein binding|
|cell activation|
|immune effector process|
|response to cytokine|
|cytoskeleton organization|
|regulation of immune response|
|positive regulation of immune system process|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|regulation of organelle organization|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|defense response|
|regulation of response to stress|
|intracellular transport|
|positive regulation of transcription, DNA-templated|
|movement of cell or subcellular component|
|protein-containing complex assembly|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|intracellular signal transduction|
|positive regulation of RNA metabolic process|
|tissue development|
|establishment of localization in cell|
|protein-containing complex subunit organization|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|vesicle-mediated transport|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|-1.74|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/694
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/26|
|bone|0/26|
|breast|0/30|
|central nervous system|0/49|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/11|
|fibroblast|0/1|
|gastric|0/14|
|kidney|0/18|
|liver|0/19|
|lung|0/72|
|lymphocyte|0/16|
|ovary|0/25|
|pancreas|0/22|
|peripheral nervous system|0/15|
|plasma cell|0/12|
|prostate|0/1|
|skin|0/20|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/28|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9117
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='WAS Expression in NALM6 Cells: 5.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>