======= WDR61 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: WDR61
  * **<color #00a2e8>Official Name</color>**: WD repeat domain 61
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=80349|80349]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9GZS3|Q9GZS3]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=WDR61&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20WDR61|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**:  N/A

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C. {ECO:0000269|PubMed:16024656, ECO:0000269|PubMed:16307923, ECO:0000269|PubMed:19952111, ECO:0000269|PubMed:20178742}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
|eIF2A|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of histone H3-K79 methylation|
|regulation of histone H3-K79 methylation|
|Ski complex|
|Cdc73/Paf1 complex|
|histone H3-K4 trimethylation|
|positive regulation of histone H3-K4 methylation|
|positive regulation of transcription elongation from RNA polymerase II promoter|
|transcriptionally active chromatin|
|regulation of histone H3-K4 methylation|
|peptidyl-lysine trimethylation|
|regulation of transcription elongation from RNA polymerase II promoter|
|positive regulation of DNA-templated transcription, elongation|
|exonucleolytic catabolism of deadenylated mRNA|
|nuclear-transcribed mRNA catabolic process, exonucleolytic|
|histone H3-K4 methylation|
|positive regulation of histone methylation|
|regulation of DNA-templated transcription, elongation|
|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|
|regulation of histone methylation|
|histone lysine methylation|
|transcription elongation from RNA polymerase II promoter|
|negative regulation of myeloid cell differentiation|
|peptidyl-lysine methylation|
|DNA-templated transcription, elongation|
|positive regulation of histone modification|
|histone methylation|
|positive regulation of chromatin organization|
|protein methylation|
|protein alkylation|
|negative regulation of hemopoiesis|
|regulation of histone modification|
|positive regulation of chromosome organization|
|regulation of chromatin organization|
|nuclear-transcribed mRNA catabolic process|
|mRNA catabolic process|
|regulation of myeloid cell differentiation|
|RNA catabolic process|
|macromolecule methylation|
|methylation|
|peptidyl-lysine modification|
|regulation of chromosome organization|
|Wnt signaling pathway|
|cell-cell signaling by wnt|
|histone modification|
|covalent chromatin modification|
|nucleobase-containing compound catabolic process|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|negative regulation of immune system process|
|aromatic compound catabolic process|
|regulation of hemopoiesis|
|organic cyclic compound catabolic process|
|transcription by RNA polymerase II|
|positive regulation of organelle organization|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|protein ubiquitination|
|mRNA metabolic process|
|chromatin organization|
|negative regulation of cell differentiation|
|protein modification by small protein conjugation|
|peptidyl-amino acid modification|
|cellular macromolecule catabolic process|
|negative regulation of developmental process|
|protein modification by small protein conjugation or removal|
|macromolecule catabolic process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|cell-cell signaling|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|positive regulation of protein modification process|
|regulation of organelle organization|
|organic cyclic compound biosynthetic process|
|positive regulation of transcription, DNA-templated|
|positive regulation of cellular protein metabolic process|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|cellular catabolic process|
|regulation of cell differentiation|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp59|UMK57 1μM R01 exp59]]|-3.27|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-2.73|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|-2.24|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|-2.15|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|-2.12|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-2.1|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|-2.08|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-2.07|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|-2.06|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-2.05|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-1.92|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-1.76|
|[[:results:exp283|Glyphosate 1000μM R06 exp283]]|-1.74|
|[[:results:exp145|PNU96415E 10μM R03 exp145]]|-1.73|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-1.72|
|[[:results:exp99|NFN1 0.4μM R03 exp99]]|-1.71|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|-1.7|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.7|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.83|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:cdc73|CDC73]]|0.476|
|[[:human genes:c:ctr9|CTR9]]|0.407|
|[[:human genes:n:narfl|NARFL]]|0.407|
|[[:human genes:i:ints5|INTS5]]|0.406|
|[[:human genes:n:nup160|NUP160]]|0.401|
|[[:human genes:i:ints8|INTS8]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 81/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|2/28|
|blood|1/28|
|bone|4/25|
|breast|12/33|
|central nervous system|6/56|
|cervix|1/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|1/15|
|kidney|5/21|
|liver|3/20|
|lung|5/75|
|lymphocyte|3/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|5/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|4/22|
|urinary tract|6/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 518
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.21 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='WDR61 Expression in NALM6 Cells: 6.21'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>