====== Chloramphenicol 2μM R00 exp12 ======
==== Mechanism of Action ====
Binds 23S rRNA of 50S ribosomal subunit, inhibits protein synthesis
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Mitochondrial Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Chloramphenicol%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 56-75-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5959|5959]]
    * **<color #00a2e8>IUPAC:</color>** %%2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** WIIZWVCIJKGZOK-RKDXNWHRSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 323.13
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=CC(=CC=C1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
    * **<color #00a2e8>Molecular Formula:</color>** C11H12Cl2N2O5
{{:chemogenomics:structures:chem-0007.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C0378
    * **<color #00a2e8>Lot #:</color>** 096K1244
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C11H12Cl2N2O5 323.0196; found 323.01899
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Chloramphenicol
    * **<color #00a2e8>Min:</color>** -5.6180; **<color #00a2e8>Max:</color>** 8.0817
{{:chemogenomics:dose_response:dr_260.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 2µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.1
    * **<color #00a2e8>Numbers of reads</color>**: 12267396


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Chloramphenicol_2uM_Round-0_exp12.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Chloramphenicol 2μM R00 exp12'>
{{:chemogenomics:cranks_plots:exp12.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.71|0.62|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.71|0.62|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.68|0.17|
|[[:human_genes:R:RPP30|RPP30]]|-2.66|0.14|
|[[:human_genes:C:CCDC42|CCDC42]]|-2.42|0.18|
|[[:human_genes:T:TEN1|TEN1]]|-2.39|0.18|
|[[:human_genes:K:KIAA0355|KIAA0355]]|-2.28|0.28|
|[[:human_genes:K:KIAA1432|KIAA1432]]|-2.20|0.31|
|[[:human_genes:K:KANK4|KANK4]]|-2.20|0.31|
|[[:human_genes:D:DDX55|DDX55]]|-2.13|0.52|
|[[:human_genes:N:NHLRC2|NHLRC2]]|-2.13|0.52|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.12|0.43|
|[[:human_genes:F:FAM50A|FAM50A]]|-2.08|0.62|
|[[:human_genes:T:TOMM5|TOMM5]]|-2.06|0.52|
|[[:human_genes:S:SHISA4|SHISA4]]|-2.02|0.62|
|[[:human_genes:G:GPS2|GPS2]]|-2.02|0.55|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.02|0.52|
|[[:human_genes:N:NCLN|NCLN]]|-2.01|0.52|
|[[:human_genes:P:PRMT5|PRMT5]]|-2.01|0.63|
|[[:human_genes:A:AHCY|AHCY]]|-1.99|0.52|
|[[:human_genes:C:CTC1|CTC1]]|-1.98|0.52|
|[[:human_genes:T:TAF1C|TAF1C]]|-1.98|0.52|
|[[:human_genes:G:GUCA2B|GUCA2B]]|-1.98|0.52|
|[[:human_genes:O:ORC3|ORC3]]|-1.97|0.61|
|[[:human_genes:F:FOXL2|FOXL2]]|-1.97|0.52|
|[[:human_genes:A:AURKB|AURKB]]|-1.96|0.63|
|[[:human_genes:G:GPR126|GPR126]]|-1.96|0.52|
|[[:human_genes:Z:ZNF488|ZNF488]]|-1.95|0.62|
|[[:human_genes:R:REEP2|REEP2]]|-1.95|0.52|
|[[:human_genes:T:TRIP6|TRIP6]]|-1.95|0.52|
|[[:human_genes:A:ANKH|ANKH]]|2.12|0.23|
|[[:human_genes:C:C2orf27B|C2orf27B]]|2.13|0.33|
|[[:human_genes:L:LRR1|LRR1]]|2.13|0.34|
|[[:human_genes:P:POLR2A|POLR2A]]|2.19|0.37|
|[[:human_genes:W:WEE1|WEE1]]|2.19|0.37|
|[[:human_genes:R:RPS5|RPS5]]|2.19|0.27|
|[[:human_genes:U:UTY|UTY]]|2.23|0.16|
|[[:human_genes:L:LOC643669|LOC643669]]|2.25|0.23|
|[[:human_genes:P:PTGFR|PTGFR]]|2.26|0.16|
|[[:human_genes:E:EIF2B4|EIF2B4]]|2.30|0.33|
|[[:human_genes:R:RPL14|RPL14]]|2.32|0.34|
|[[:human_genes:S:SYNPR|SYNPR]]|2.35|0.12|
|[[:human_genes:P:PSME3|PSME3]]|2.38|0.12|
|[[:human_genes:S:SLC25A36|SLC25A36]]|2.39|0.12|
|[[:human_genes:R:RPL8|RPL8]]|2.40|0.49|
|[[:human_genes:M:MGAT4D|MGAT4D]]|2.45|0.47|
|[[:human_genes:T:TPR|TPR]]|2.47|0.12|
|[[:human_genes:A:ANKRD40|ANKRD40]]|2.51|0.16|
|[[:human_genes:Z:ZNF713|ZNF713]]|2.53|0.16|
|[[:human_genes:A:AGK|AGK]]|2.54|0.08|
|[[:human_genes:A:ATF6|ATF6]]|2.54|0.12|
|[[:human_genes:N:NGLY1|NGLY1]]|2.57|0.08|
|[[:human_genes:P:PRPF8|PRPF8]]|2.59|0.34|
|[[:human_genes:A:ANKRD36|ANKRD36]]|2.60|0.08|
|[[:human_genes:C:COPE|COPE]]|2.68|0.27|
|[[:human_genes:R:RPS12|RPS12]]|2.75|0.08|
|[[:human_genes:S:SCAF1|SCAF1]]|2.82|0.36|
|[[:human_genes:C:CCT4|CCT4]]|2.96|0.34|
|[[:human_genes:R:RBM39|RBM39]]|3.09|0.33|
|[[:human_genes:S:SUPT6H|SUPT6H]]|3.25|0.16|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|0.07|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|0.068|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|0.054|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp295|Pyronaridine 1μM R06 exp295]]|0.054|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|0.081|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp11|CCCP 1μM R00 exp11]]|0.075|<button type="primary" size="small" modal="6">View</button>|
</modal>


<modal id="1" size="lg" title="Chloramphenicol 2μM R00 exp12  vs  Nocodazole 0.02μM R00 exp23">
{{:chemogenomics:comparison_plots:exp12_vs_exp23.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Chloramphenicol 2μM R00 exp12  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp12_vs_exp31.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Chloramphenicol 2μM R00 exp12  vs  Deguelin 0.15μM R05 exp209">
{{:chemogenomics:comparison_plots:exp12_vs_exp209.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Chloramphenicol 2μM R00 exp12  vs  Pyronaridine 1μM R06 exp295">
{{:chemogenomics:comparison_plots:exp12_vs_exp295.png?nolink |}}
</modal>

<modal id="5" size="lg" title="CCCP 0.1μM R00 exp10  vs  Chloramphenicol 2μM R00 exp12">
{{:chemogenomics:comparison_plots:exp10_vs_exp12.png?nolink |}}
</modal>

<modal id="6" size="lg" title="CCCP 1μM R00 exp11  vs  Chloramphenicol 2μM R00 exp12">
{{:chemogenomics:comparison_plots:exp11_vs_exp12.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|CST complex|637.73|TEN1,CTC1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>