====== Chloramphenicol 20μM R00 exp13 ======
==== Mechanism of Action ====
Binds 23S rRNA of 50S ribosomal subunit, inhibits protein synthesis
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Mitochondrial Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Chloramphenicol%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 56-75-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5959|5959]]
    * **<color #00a2e8>IUPAC:</color>** %%2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** WIIZWVCIJKGZOK-RKDXNWHRSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 323.13
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=CC(=CC=C1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
    * **<color #00a2e8>Molecular Formula:</color>** C11H12Cl2N2O5
{{:chemogenomics:structures:chem-0007.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C0378
    * **<color #00a2e8>Lot #:</color>** 096K1244
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C11H12Cl2N2O5 323.0196; found 323.01899
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Chloramphenicol
    * **<color #00a2e8>Min:</color>** -5.6180; **<color #00a2e8>Max:</color>** 8.0817
{{:chemogenomics:dose_response:dr_260.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.8
    * **<color #00a2e8>Numbers of reads</color>**: 10775991


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/18|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Chloramphenicol_20uM_Round-0_exp13.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Chloramphenicol 20μM R00 exp13'>
{{:chemogenomics:cranks_plots:exp13.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GOT1|GOT1]]|-4.37|<0.01|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.91|0.01|
|[[:human_genes:H:HARS|HARS]]|-2.82|0.37|
|[[:human_genes:A:AURKB|AURKB]]|-2.76|0.37|
|[[:human_genes:P:PDXK|PDXK]]|-2.72|0.03|
|[[:human_genes:P:PRMT5|PRMT5]]|-2.68|0.44|
|[[:human_genes:O:ORC3|ORC3]]|-2.49|0.37|
|[[:human_genes:K:KDM2B|KDM2B]]|-2.32|0.37|
|[[:human_genes:U:UNK|UNK]]|-2.25|0.37|
|[[:human_genes:H:HPRT1|HPRT1]]|-2.23|0.37|
|[[:human_genes:F:FAM132B|FAM132B]]|-2.23|0.37|
|[[:human_genes:R:RACGAP1|RACGAP1]]|-2.16|0.37|
|[[:human_genes:U:UROD|UROD]]|-2.15|0.44|
|[[:human_genes:C:C17orf82|C17orf82]]|-2.11|0.37|
|[[:human_genes:S:SLC4A7|SLC4A7]]|-2.09|0.37|
|[[:human_genes:X:XPO5|XPO5]]|-2.08|0.51|
|[[:human_genes:C:CPXM1|CPXM1]]|-2.05|0.37|
|[[:human_genes:E:ECHS1|ECHS1]]|-2.05|0.37|
|[[:human_genes:Z:ZFAND4|ZFAND4]]|-2.02|0.37|
|[[:human_genes:P:PHF15|PHF15]]|-2.00|0.37|
|[[:human_genes:D:DGAT1|DGAT1]]|-2.00|0.42|
|[[:human_genes:H:HADH|HADH]]|-2.00|0.37|
|[[:human_genes:K:KIF23|KIF23]]|-1.99|0.37|
|[[:human_genes:C:CARKD|CARKD]]|-1.99|0.37|
|[[:human_genes:Y:YTHDF1|YTHDF1]]|-1.98|0.37|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-1.98|0.42|
|[[:human_genes:M:MAP6D1|MAP6D1]]|-1.97|0.42|
|[[:human_genes:A:ADRA1B|ADRA1B]]|-1.97|0.42|
|[[:human_genes:P:PCK1|PCK1]]|-1.96|0.43|
|[[:human_genes:P:PIGY|PIGY]]|-1.95|0.42|
|[[:human_genes:A:ACAD9|ACAD9]]|2.29|0.06|
|[[:human_genes:S:SMG7|SMG7]]|2.31|0.39|
|[[:human_genes:N:NDUFS1|NDUFS1]]|2.32|0.10|
|[[:human_genes:P:PRKCA|PRKCA]]|2.32|0.06|
|[[:human_genes:C:CHMP2A|CHMP2A]]|2.38|0.37|
|[[:human_genes:S:SLC25A52|SLC25A52]]|2.40|0.04|
|[[:human_genes:A:ATP5E|ATP5E]]|2.40|0.12|
|[[:human_genes:N:NFE2L1|NFE2L1]]|2.42|0.07|
|[[:human_genes:C:CD79B|CD79B]]|2.43|0.11|
|[[:human_genes:R:RPL23|RPL23]]|2.46|0.26|
|[[:human_genes:C:CWC22|CWC22]]|2.52|0.03|
|[[:human_genes:M:MTIF2|MTIF2]]|2.53|0.05|
|[[:human_genes:A:ATP5O|ATP5O]]|2.53|0.03|
|[[:human_genes:T:TMEM165|TMEM165]]|2.57|0.04|
|[[:human_genes:A:ATP5A1|ATP5A1]]|2.61|0.03|
|[[:human_genes:C:CKAP5|CKAP5]]|2.62|0.29|
|[[:human_genes:V:VCP|VCP]]|2.63|0.03|
|[[:human_genes:A:ATP5L|ATP5L]]|2.65|0.05|
|[[:human_genes:I:IPPK|IPPK]]|2.67|0.02|
|[[:human_genes:N:NDUFC2|NDUFC2]]|2.69|0.02|
|[[:human_genes:N:NR2C2AP|NR2C2AP]]|2.71|0.03|
|[[:human_genes:K:KIF11|KIF11]]|2.73|0.37|
|[[:human_genes:S:SNRNP70|SNRNP70]]|2.74|0.03|
|[[:human_genes:M:MYCBP2|MYCBP2]]|2.75|0.03|
|[[:human_genes:I:IPMK|IPMK]]|2.80|0.02|
|[[:human_genes:S:STARD7|STARD7]]|2.81|0.02|
|[[:human_genes:M:MDH1|MDH1]]|2.97|0.01|
|[[:human_genes:G:G3BP1|G3BP1]]|3.18|<0.01|
|[[:human_genes:I:ITPK1|ITPK1]]|3.31|<0.01|
|[[:human_genes:S:SLC25A51|SLC25A51]]|3.92|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|0.107|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|0.095|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|0.094|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|0.09|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|0.084|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|0.076|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|0.073|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|0.065|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp380|NMS-873 0.07μM R07 exp380]]|0.052|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.052|<button type="primary" size="small" modal="10">View</button>|
</modal>


<modal id="1" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  QNZ 0.01μM R08 exp439">
{{:chemogenomics:comparison_plots:exp13_vs_exp439.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Mubritinib 0.2μM R01 exp48">
{{:chemogenomics:comparison_plots:exp13_vs_exp48.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Mubritinib 0.2μM R02 exp65">
{{:chemogenomics:comparison_plots:exp13_vs_exp65.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Phenformin 20μM R05 exp212">
{{:chemogenomics:comparison_plots:exp13_vs_exp212.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  QNZ 0.01μM R05 exp241">
{{:chemogenomics:comparison_plots:exp13_vs_exp241.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Rotenone 0.07μM R08 exp431">
{{:chemogenomics:comparison_plots:exp13_vs_exp431.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Deguelin 0.15μM R05 exp209">
{{:chemogenomics:comparison_plots:exp13_vs_exp209.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp13_vs_exp25.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  NMS-873 0.07μM R07 exp380">
{{:chemogenomics:comparison_plots:exp13_vs_exp380.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Rapamycin 1μM R00 exp29">
{{:chemogenomics:comparison_plots:exp13_vs_exp29.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|mitotic spindle midzone assembly|177.65|AURKB,RACGAP1,KIF23|
|spindle elongation|148.04|AURKB,RACGAP1,KIF23|
|spindle midzone assembly|136.66|AURKB,RACGAP1,KIF23|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|proton-transporting ATP synthase activity, rotational mechanism|157.91|ATP5MG,ATP5F1A,ATP5PO,ATP5F1E|
|mitochondrial proton-transporting ATP synthase complex|147.39|ATP5MG,ATP5F1A,ATP5PO,ATP5F1E|
|proton motive force-driven mitochondrial ATP synthesis|62.57|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|proton motive force-driven ATP synthesis|55.27|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|ATP biosynthetic process|47.37|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|purine ribonucleoside triphosphate biosynthetic process|40.94|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|purine nucleoside triphosphate biosynthetic process|40.44|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|oxidative phosphorylation|39.08|SLC25A51,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|ribonucleoside triphosphate biosynthetic process|38.56|NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|ATP metabolic process|38.31|NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|aerobic respiration|31.36|SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|purine ribonucleoside triphosphate metabolic process|30.95|NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|purine nucleoside triphosphate metabolic process|29.76|NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|ribonucleoside triphosphate metabolic process|29.53|NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|nucleoside triphosphate metabolic process|26.50|NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|cellular respiration|26.16|SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|energy derivation by oxidation of organic compounds|18.90|SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1|
|mitochondrial membrane|9.39|SLC25A51,STARD7,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,SLC25A52,PRKCA,NDUFS1,ALAS1,ACAD9|
|mitochondrial envelope|8.82|SLC25A51,STARD7,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,SLC25A52,PRKCA,NDUFS1,ALAS1,ACAD9|
</modal>