====== Cycloheximide 0.2μM R00 exp15 ======
==== Mechanism of Action ====
Translation elongation inhibitor, binds 60S ribosomal subunit
  * **<color #00a2e8>Class / Subclass 1:</color>** Proteostasis / Translation Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Environmental Stresses / Toxin



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Cycloheximide%%
    * **<color #00a2e8>Synonyms:</color>** Naramycin A; Actidione; CHX
    * **<color #00a2e8>CAS #:</color>** 66-81-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/6197|6197]]
    * **<color #00a2e8>IUPAC:</color>** %%4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** YPHMISFOHDHNIV-FSZOTQKASA-N
    * **<color #00a2e8>Molecular Weight:</color>** 281.35
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]1C[C@@H](C(=O)[C@@H](C1)[C@@H](CC2CC(=O)NC(=O)C2)O)C
    * **<color #00a2e8>Molecular Formula:</color>** C15H23NO4
{{:chemogenomics:structures:chem-0008.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C7698
    * **<color #00a2e8>Lot #:</color>** MKBR7146V
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C15H23NO4 282.16998; found 282.17004
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Cycloheximide
    * **<color #00a2e8>Min:</color>** 3.7773; **<color #00a2e8>Max:</color>** 80.9065
{{:chemogenomics:dose_response:dr_261.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.1270    |
| IC30  |0.3675    |
| IC40  |0.8782    |
| IC50  |1.9535    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 200nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.6
    * **<color #00a2e8>Numbers of reads</color>**: 9929747


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Cycloheximide_0.2uM_Round-0_exp15.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Cycloheximide 0.2μM R00 exp15'>
{{:chemogenomics:cranks_plots:exp15.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SETD6|SETD6]]|-4.60|<0.01|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-3.41|0.27|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-3.39|0.27|
|[[:human_genes:D:DDX39A|DDX39A]]|-3.09|<0.01|
|[[:human_genes:C:CD2BP2|CD2BP2]]|-2.76|0.02|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.58|0.10|
|[[:human_genes:D:DDX6|DDX6]]|-2.53|0.07|
|[[:human_genes:A:AHCY|AHCY]]|-2.50|0.07|
|[[:human_genes:O:ORC3|ORC3]]|-2.38|0.21|
|[[:human_genes:C:CALML3|CALML3]]|-2.37|0.18|
|[[:human_genes:D:DYSF|DYSF]]|-2.32|0.15|
|[[:human_genes:S:SCO2|SCO2]]|-2.28|0.15|
|[[:human_genes:D:DRG2|DRG2]]|-2.28|0.15|
|[[:human_genes:P:PTRH2|PTRH2]]|-2.27|0.21|
|[[:human_genes:K:KRTAP10-3|KRTAP10-3]]|-2.23|0.21|
|[[:human_genes:C:CDK2|CDK2]]|-2.18|0.26|
|[[:human_genes:K:KRT71|KRT71]]|-2.15|0.21|
|[[:human_genes:O:ODC1|ODC1]]|-2.15|0.21|
|[[:human_genes:G:GGPS1|GGPS1]]|-2.12|0.30|
|[[:human_genes:F:FBXO11|FBXO11]]|-2.12|0.21|
|[[:human_genes:T:TOMM34|TOMM34]]|-2.12|0.21|
|[[:human_genes:A:ASB3|ASB3]]|-2.11|0.21|
|[[:human_genes:M:MEF2A|MEF2A]]|-2.08|0.35|
|[[:human_genes:C:CFAP45|CFAP45]]|-2.08|0.67|
|[[:human_genes:N:NEMF|NEMF]]|-2.07|0.35|
|[[:human_genes:T:TP53I13|TP53I13]]|-2.03|0.38|
|[[:human_genes:A:ATF7IP|ATF7IP]]|-2.02|0.28|
|[[:human_genes:D:DCAF15|DCAF15]]|-2.00|0.43|
|[[:human_genes:P:PRDM10|PRDM10]]|-1.99|0.44|
|[[:human_genes:Z:ZNF598|ZNF598]]|-1.95|0.46|
|[[:human_genes:T:TNFRSF10D|TNFRSF10D]]|2.15|0.27|
|[[:human_genes:P:PSMD1|PSMD1]]|2.15|0.55|
|[[:human_genes:Z:ZNF28|ZNF28]]|2.16|0.27|
|[[:human_genes:N:NSL1|NSL1]]|2.16|0.27|
|[[:human_genes:G:GPR183|GPR183]]|2.16|0.27|
|[[:human_genes:T:TIMM23B|TIMM23B]]|2.16|0.44|
|[[:human_genes:M:MPC2|MPC2]]|2.17|0.27|
|[[:human_genes:C:COPG1|COPG1]]|2.19|0.43|
|[[:human_genes:N:NUP153|NUP153]]|2.20|0.30|
|[[:human_genes:R:RRP1|RRP1]]|2.21|0.27|
|[[:human_genes:C:CCDC90B|CCDC90B]]|2.21|0.27|
|[[:human_genes:K:KRAS|KRAS]]|2.22|0.27|
|[[:human_genes:V:VASN|VASN]]|2.22|0.27|
|[[:human_genes:D:DNM2|DNM2]]|2.24|0.49|
|[[:human_genes:E:ELOVL5|ELOVL5]]|2.24|0.27|
|[[:human_genes:R:RPL8|RPL8]]|2.25|0.57|
|[[:human_genes:T:TMEM165|TMEM165]]|2.25|0.27|
|[[:human_genes:W:WDR43|WDR43]]|2.27|0.40|
|[[:human_genes:U:USP27X|USP27X]]|2.30|0.57|
|[[:human_genes:M:MARVELD1|MARVELD1]]|2.30|0.57|
|[[:human_genes:P:PSMD7|PSMD7]]|2.32|0.40|
|[[:human_genes:D:DDI2|DDI2]]|2.45|0.25|
|[[:human_genes:B:BCL2L1|BCL2L1]]|2.52|0.27|
|[[:human_genes:R:RPS6|RPS6]]|2.57|0.40|
|[[:human_genes:P:PLK4|PLK4]]|2.64|0.29|
|[[:human_genes:D:DTL|DTL]]|2.68|0.27|
|[[:human_genes:S:SCAF1|SCAF1]]|2.79|0.43|
|[[:human_genes:C:COL28A1|COL28A1]]|3.00|0.17|
|[[:human_genes:K:KIF11|KIF11]]|3.05|0.36|
|[[:human_genes:C:CKAP5|CKAP5]]|3.28|0.27|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|0.071|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp235|Geldanamycin 0.01μM R05 exp235]]|0.065|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|0.061|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.058|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.056|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|0.074|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|0.059|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|0.051|<button type="primary" size="small" modal="8">View</button>|
</modal>


<modal id="1" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp15_vs_exp31.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Geldanamycin 0.01μM R05 exp235">
{{:chemogenomics:comparison_plots:exp15_vs_exp235.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp15_vs_exp32.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Rapamycin 1μM R00 exp29">
{{:chemogenomics:comparison_plots:exp15_vs_exp29.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp15_vs_exp38.png?nolink |}}
</modal>

<modal id="6" size="lg" title="CCCP 0.1μM R00 exp10  vs  Cycloheximide 0.2μM R00 exp15">
{{:chemogenomics:comparison_plots:exp10_vs_exp15.png?nolink |}}
</modal>

<modal id="7" size="lg" title="5-Fluorouracil 2μM R00 exp1  vs  Cycloheximide 0.2μM R00 exp15">
{{:chemogenomics:comparison_plots:exp1_vs_exp15.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Cycloheximide 0.2μM R00 exp15">
{{:chemogenomics:comparison_plots:exp15_vs_exp5.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>