====== BI-D1870 2μM R04 exp162 ======
==== Mechanism of Action ====
Inhibits ribosomal p90 S6 Kinase, active against RSK1/2/3/4 isoforms
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%2-((3,5-Difluoro-4-hydroxyphenyl)amino)-8-isopentyl-5,7-dimethyl-7,8-dihydropteridin-6(5H)-one%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 501437-28-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/25023738|25023738]]
    * **<color #00a2e8>IUPAC:</color>** %%2-(3,5-difluoro-4-hydroxyanilino)-5,7-dimethyl-8-(3-methylbutyl)-7H-pteridin-6-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C19H23F2N5O2/c1-10(2)5-6-26-11(3)18(28)25(4)15-9-22-19(24-17(15)26)23-12-7-13(20)16(27)14(21)8-12/h7-11,27H,5-6H2,1-4H3,(H,22,23,24)
    * **<color #00a2e8>INCHI Key:</color>** DTEKTGDVSARYDS-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 391.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC1C(=O)N(C2=CN=C(N=C2N1CCC(C)C)NC3=CC(=C(C(=C3)F)O)F)C
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C19H23F2N5O2
{{:chemogenomics:structures:chem-0042.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Selleck Chemicals
    * **<color #00a2e8>Catalog #:</color>** S2843
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C19H23F2N5O2 392.18926; found 392.19055
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** BI-D1870
    * **<color #00a2e8>Min:</color>** -18.9777; **<color #00a2e8>Max:</color>** 94.2694
{{:chemogenomics:dose_response:dr_3.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |2.5715    |
| IC30  |2.6985    |
| IC40  |2.8073    |
| IC50  |2.9110    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 04
    * **<color #00a2e8>Dose</color>**: 2µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.0
    * **<color #00a2e8>Numbers of reads</color>**: 16218675


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|15/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/BI-D1870_2uM_Round-4_exp162.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='BI-D1870 2μM R04 exp162'>
{{:chemogenomics:cranks_plots:exp162.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:T:TCF4|TCF4]]|-3.97|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.18|<0.01|
|[[:human_genes:S:SKA3|SKA3]]|-3.06|<0.01|
|[[:human_genes:C:CENPQ|CENPQ]]|-3.01|<0.01|
|[[:human_genes:C:CCNB1|CCNB1]]|-2.88|0.01|
|[[:human_genes:V:VEZT|VEZT]]|-2.78|0.01|
|[[:human_genes:K:KIF18A|KIF18A]]|-2.73|0.01|
|[[:human_genes:S:SKA1|SKA1]]|-2.72|0.01|
|[[:human_genes:M:MSI2|MSI2]]|-2.70|0.01|
|[[:human_genes:S:SPEN|SPEN]]|-2.66|0.01|
|[[:human_genes:R:RBM14|RBM14]]|-2.65|0.03|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.60|0.06|
|[[:human_genes:M:METTL14|METTL14]]|-2.57|0.07|
|[[:human_genes:C:CTPS1|CTPS1]]|-2.56|0.37|
|[[:human_genes:E:ELF2|ELF2]]|-2.51|0.03|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|-2.51|0.05|
|[[:human_genes:M:MCTS1|MCTS1]]|-2.48|0.03|
|[[:human_genes:E:ELAVL1|ELAVL1]]|-2.47|0.03|
|[[:human_genes:B:BRD1|BRD1]]|-2.43|0.04|
|[[:human_genes:P:PRC1|PRC1]]|-2.35|0.09|
|[[:human_genes:A:AGGF1|AGGF1]]|-2.33|0.09|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-2.32|0.06|
|[[:human_genes:N:NCAPH2|NCAPH2]]|-2.31|0.09|
|[[:human_genes:P:PRUNE|PRUNE]]|-2.31|0.06|
|[[:human_genes:L:LSM10|LSM10]]|-2.30|0.06|
|[[:human_genes:P:PIAS4|PIAS4]]|-2.28|0.07|
|[[:human_genes:N:NUTF2|NUTF2]]|-2.25|0.48|
|[[:human_genes:P:PCIF1|PCIF1]]|-2.22|0.09|
|[[:human_genes:S:SNF8|SNF8]]|-2.20|0.15|
|[[:human_genes:P:PRKACA|PRKACA]]|-2.19|0.09|
|[[:human_genes:Z:ZC3H15|ZC3H15]]|1.99|0.38|
|[[:human_genes:S:SPOP|SPOP]]|1.99|0.38|
|[[:human_genes:S:SFXN1|SFXN1]]|2.00|0.38|
|[[:human_genes:K:KIF2A|KIF2A]]|2.01|0.38|
|[[:human_genes:T:TRIM37|TRIM37]]|2.02|0.38|
|[[:human_genes:C:CKS2|CKS2]]|2.02|0.38|
|[[:human_genes:A:ABHD12B|ABHD12B]]|2.04|0.38|
|[[:human_genes:G:GPBP1|GPBP1]]|2.08|0.38|
|[[:human_genes:F:FAM186A|FAM186A]]|2.08|0.40|
|[[:human_genes:R:RBM10|RBM10]]|2.11|0.38|
|[[:human_genes:T:TAZ|TAZ]]|2.13|0.68|
|[[:human_genes:W:WDR46|WDR46]]|2.15|0.47|
|[[:human_genes:D:DNM2|DNM2]]|2.15|0.68|
|[[:human_genes:Y:YLPM1|YLPM1]]|2.16|0.47|
|[[:human_genes:L:LOC643669|LOC643669]]|2.16|0.47|
|[[:human_genes:P:POLA1|POLA1]]|2.18|0.67|
|[[:human_genes:X:XPO4|XPO4]]|2.18|0.38|
|[[:human_genes:S:SNRNP70|SNRNP70]]|2.19|0.38|
|[[:human_genes:S:SMU1|SMU1]]|2.20|0.50|
|[[:human_genes:M:MORC2|MORC2]]|2.21|0.38|
|[[:human_genes:M:MCL1|MCL1]]|2.26|0.38|
|[[:human_genes:P:PCDHGB4|PCDHGB4]]|2.30|0.38|
|[[:human_genes:S:SIRT6|SIRT6]]|2.30|0.38|
|[[:human_genes:B:BCAS3|BCAS3]]|2.31|0.30|
|[[:human_genes:C:CDK8|CDK8]]|2.32|0.30|
|[[:human_genes:C:CFAP45|CFAP45]]|2.40|0.65|
|[[:human_genes:S:ST7|ST7]]|2.41|0.30|
|[[:human_genes:D:DDX21|DDX21]]|2.51|0.61|
|[[:human_genes:S:SRSF4|SRSF4]]|2.55|0.29|
|[[:human_genes:W:WDR75|WDR75]]|2.56|0.32|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|0.075|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|0.062|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|0.06|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.06|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.059|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|0.054|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.06|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.06|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|0.051|<button type="primary" size="small" modal="10">View</button>|
</modal>


<modal id="1" size="lg" title="BI-D1870 2μM R04 exp162  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp162.png?nolink |}}
</modal>

<modal id="2" size="lg" title="BI-D1870 2μM R04 exp162  vs  Epigallocatechin gallate 20μM R05 exp230">
{{:chemogenomics:comparison_plots:exp162_vs_exp230.png?nolink |}}
</modal>

<modal id="3" size="lg" title="BI-D1870 2μM R04 exp162  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp162.png?nolink |}}
</modal>

<modal id="4" size="lg" title="BI-D1870 2μM R04 exp162  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp162_vs_exp184.png?nolink |}}
</modal>

<modal id="5" size="lg" title="BI-D1870 2μM R04 exp162  vs  Combretastatin A4 0.002 to 0.003μM day4 R04 exp179">
{{:chemogenomics:comparison_plots:exp162_vs_exp179.png?nolink |}}
</modal>

<modal id="6" size="lg" title="BI-D1870 2μM R04 exp162  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp162_vs_exp243.png?nolink |}}
</modal>

<modal id="7" size="lg" title="BI-D1870 2μM R04 exp162  vs  I-BRD9 4μM R07 exp365">
{{:chemogenomics:comparison_plots:exp162_vs_exp365.png?nolink |}}
</modal>

<modal id="8" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  BI-D1870 2μM R04 exp162">
{{:chemogenomics:comparison_plots:exp162_vs_exp311.png?nolink |}}
</modal>

<modal id="9" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  BI-D1870 2μM R04 exp162">
{{:chemogenomics:comparison_plots:exp162_vs_exp312.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BI-2536 0.0042μM R03 exp95  vs  BI-D1870 2μM R04 exp162">
{{:chemogenomics:comparison_plots:exp162_vs_exp95.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|outer kinetochore|93.46|SKA3,CCNB1,SKA1|
|spindle microtubule|30.36|SKA3,KIF18A,SKA1,PRC1|
|intercellular bridge|26.60|SKA1,PRC1,NCAPH2,PCIF1|
|kinetochore|18.16|SKA3,CENPQ,CCNB1,KIF18A,SKA1|
|condensed chromosome, centromeric region|17.01|SKA3,CENPQ,CCNB1,KIF18A,SKA1|
|chromosome, centromeric region|15.11|PDS5B,SKA3,CENPQ,CCNB1,KIF18A,SKA1|
|condensed chromosome|13.11|SKA3,CENPQ,CCNB1,KIF18A,SKA1,NCAPH2|
|chromosome segregation|12.63|SKA3,CENPQ,CCNB1,KIF18A,SKA1,PRC1,NCAPH2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>