====== DABN 20μM R00 exp17 ======
==== Mechanism of Action ====
Unknown
* **Class / Subclass 1:** Uncharacterized Mechanism / Chemical Screen Hit
==== Technical Notes ====
* **PubChem Name:** %%[1,1'-Binaphthalene]-2,2'-diamine%%
* **Synonyms:** N/A
* **CAS #:** 4488-22-6
* **PubChem CID:** [[https://pubchem.ncbi.nlm.nih.gov/compound/20571|20571]]
* **IUPAC:** %%1-(2-aminonaphthalen-1-yl)naphthalen-2-amine%%
* **INCHI Name:** InChI=1S/C20H16N2/c21-17-11-9-13-5-1-3-7-15(13)19(17)20-16-8-4-2-6-14(16)10-12-18(20)22/h1-12H,21-22H2
* **INCHI Key:** DDAPSNKEOHDLKB-UHFFFAOYSA-N
* **Molecular Weight:** 284.4
* **Canonical SMILES:** C1=CC=C2C(=C1)C=CC(=C2C3=C(C=CC4=CC=CC=C43)N)N
* **Isomeric SMILES:** N/A
* **Molecular Formula:** C20H16N2
{{:chemogenomics:structures:chem-0009.svg?nolink}}
* **Supplier Name:** Chem-Impex International
* **Catalog #:** 24623
* **Lot #:** 001702-20140303
* **HRMS (ESI-TOF) m/z:** (M+H)+ Calcd for C20H16N2 285.13862; found 285.13894
* **Platform ID:** DABN
* **Min:** -10.2228; **Max:** 85.8979
{{:chemogenomics:dose_response:dr_262.png?nolink&500 |}}
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^ IC ^ Concentration (µM) ^
| IC10 |N/A |
| IC20 |6.0249 |
| IC30 |6.1377 |
| IC40 |6.2318 |
| IC50 |6.3193 |
| IC60 |N/A |
| IC70 |N/A |
| IC80 |N/A |
| IC90 |N/A |
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==== Screen Summary ====
* **Round**: 00
* **Dose**: 20µM
* **Days of incubation**: 8
* **Doublings**: 0.4
* **Numbers of reads**: 11551454
==== Screen Results ====
^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|10/9|[[https://files.tyerslab.com/files/public/chemogenomics/cranks/DABN_20uM_Round-0_exp17.txt|Scores]]||||||
{{:chemogenomics:cranks_plots:exp17.png?nolink}}
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ATP6V1A|ATP6V1A]]|-3.93|<0.01|
|[[:human_genes:A:ATP6V1B2|ATP6V1B2]]|-3.88|<0.01|
|[[:human_genes:S:SPTLC2|SPTLC2]]|-3.71|<0.01|
|[[:human_genes:A:AURKB|AURKB]]|-3.51|0.02|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-3.40|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.35|<0.01|
|[[:human_genes:D:DDX3X|DDX3X]]|-3.09|<0.01|
|[[:human_genes:C:CNOT2|CNOT2]]|-3.00|<0.01|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.93|0.30|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.91|0.30|
|[[:human_genes:E:EP300|EP300]]|-2.89|0.11|
|[[:human_genes:B:BRD2|BRD2]]|-2.88|<0.01|
|[[:human_genes:A:ATP6AP1|ATP6AP1]]|-2.77|0.35|
|[[:human_genes:X:XAB2|XAB2]]|-2.74|0.12|
|[[:human_genes:S:SRBD1|SRBD1]]|-2.70|0.12|
|[[:human_genes:P:PSMB4|PSMB4]]|-2.67|0.08|
|[[:human_genes:L:LRP10|LRP10]]|-2.59|0.09|
|[[:human_genes:H:HNRNPCL1|HNRNPCL1]]|-2.53|0.10|
|[[:human_genes:A:ATP6V0D1|ATP6V0D1]]|-2.53|0.12|
|[[:human_genes:C:COPA|COPA]]|-2.53|0.21|
|[[:human_genes:B:BAGE|BAGE]]|-2.52|0.44|
|[[:human_genes:B:BAGE3|BAGE3]]|-2.51|0.44|
|[[:human_genes:P:PRIM1|PRIM1]]|-2.51|0.44|
|[[:human_genes:B:BAGE2|BAGE2]]|-2.50|0.44|
|[[:human_genes:B:BAGE5|BAGE5]]|-2.49|0.44|
|[[:human_genes:B:BAGE4|BAGE4]]|-2.48|0.44|
|[[:human_genes:E:EGFL8|EGFL8]]|-2.47|0.04|
|[[:human_genes:I:INCENP|INCENP]]|-2.44|0.09|
|[[:human_genes:R:RNF103-CHMP3|RNF103-CHMP3]]|-2.42|0.09|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.41|0.11|
|[[:human_genes:T:TLE4|TLE4]]|2.05|0.39|
|[[:human_genes:I:ITIH2|ITIH2]]|2.09|0.25|
|[[:human_genes:H:HSPD1|HSPD1]]|2.09|0.16|
|[[:human_genes:R:RIMS2|RIMS2]]|2.12|0.15|
|[[:human_genes:M:MED23|MED23]]|2.14|0.14|
|[[:human_genes:C:CSNK2B|CSNK2B]]|2.15|0.23|
|[[:human_genes:S:SKIL|SKIL]]|2.16|0.14|
|[[:human_genes:A:ATG16L1|ATG16L1]]|2.18|0.13|
|[[:human_genes:T:TRIAP1|TRIAP1]]|2.22|0.13|
|[[:human_genes:P:PABPC4|PABPC4]]|2.24|0.13|
|[[:human_genes:L:LDB2|LDB2]]|2.24|0.15|
|[[:human_genes:C:CRLS1|CRLS1]]|2.25|0.13|
|[[:human_genes:G:GPC3|GPC3]]|2.27|0.12|
|[[:human_genes:M:MTOR|MTOR]]|2.29|0.23|
|[[:human_genes:C:CCDC117|CCDC117]]|2.32|0.13|
|[[:human_genes:B:BTBD9|BTBD9]]|2.32|0.13|
|[[:human_genes:T:TIMM23B|TIMM23B]]|2.35|0.39|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|2.43|0.14|
|[[:human_genes:R:RBM14|RBM14]]|2.44|0.14|
|[[:human_genes:R:RAD1|RAD1]]|2.44|0.05|
|[[:human_genes:A:APAF1|APAF1]]|2.73|0.01|
|[[:human_genes:H:HCCS|HCCS]]|2.73|0.06|
|[[:human_genes:P:PELO|PELO]]|2.91|<0.01|
|[[:human_genes:M:MAD1L1|MAD1L1]]|3.33|<0.01|
|[[:human_genes:B:BUB1B|BUB1B]]|3.77|<0.01|
|[[:human_genes:Z:ZW10|ZW10]]|4.17|<0.01|
|[[:human_genes:M:MAD2L1|MAD2L1]]|4.79|<0.01|
|[[:human_genes:B:BUB3|BUB3]]|4.85|<0.01|
|[[:human_genes:T:TRIP13|TRIP13]]|5.07|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|6.66|<0.01|
^Screen^Correlation^Plot^
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|0.113||
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|0.111||
|[[:results:exp19|Etoposide 1μM R00 exp19]]|0.11||
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.094||
|[[:results:exp26|Oligomycin-A 20μM R00 exp26]]|0.092||
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|0.083||
|[[:results:exp34|Rotenone 20μM R00 exp34]]|0.079||
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|0.078||
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|0.074||
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.074||
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|0.071||
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|0.057||
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|0.056||
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.054||
|[[:results:exp515|PU-H71 1μM R08 exp515]]|0.053||
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.053||
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.052||
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.051||
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|0.119||
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|0.098||
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.066||
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|0.064||
|[[:results:exp8|Brefeldin A 0.02μM R00 exp8]]|0.056||
|[[:results:exp4|Actinomycin-D 0.01μM R00 exp4]]|0.056||
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|0.053||
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|0.052||
{{:chemogenomics:comparison_plots:exp17_vs_exp28.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp24.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp19.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp33.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp26.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp22.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp34.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp64.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp531.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp208.png?nolink |}}
{{:chemogenomics:comparison_plots:exp108_vs_exp17.png?nolink |}}
{{:chemogenomics:comparison_plots:exp126_vs_exp17.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp256.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp199.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp515.png?nolink |}}
{{:chemogenomics:comparison_plots:exp111_vs_exp17.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp93.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp478.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp7.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp2.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp98.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp3.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp8.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp4.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp96.png?nolink |}}
{{:chemogenomics:comparison_plots:exp17_vs_exp343.png?nolink |}}
^GO Term^Fold Change^Genes^
|cellular response to increased oxygen levels|205.55|ATP6V1A,ATP6V0A2,ATP6AP1|
|synaptic vesicle lumen acidification|200.98|ATP6V1A,ATP6V1B2,ATP6AP1,ATP6V0D1|
|proton-transporting V-type ATPase complex|139.57|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|ATPase-coupled ion transmembrane transporter activity|131.08|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1|
|proton-transporting ATPase activity, rotational mechanism|131.08|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1|
|ATPase activity, coupled to transmembrane movement of ions, rotational mechanism|131.08|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1|
|vacuolar proton-transporting V-type ATPase complex|125.61|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1|
|response to increased oxygen levels|120.59|ATP6V1A,ATP6V0A2,ATP6AP1,ATP6V0D1|
|vacuolar acidification|114.19|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|proton-transporting two-sector ATPase complex|83.74|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|intracellular pH reduction|73.89|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|ATPase-coupled monoatomic cation transmembrane transporter activity|59.11|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1|
|intracellular iron ion homeostasis|44.33|ATP6V1A,ATP6V0A2,ATP6AP1,ATP6V0D1|
|regulation of intracellular pH|43.32|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|regulation of cellular pH|40.96|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|regulation of pH|36.95|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|proton transmembrane transport|28.55|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|ATPase complex|26.92|ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1|
|coated vesicle membrane|19.53|ATP6V1A,ATP6V1B2,ATP6AP1,ATP6V0D1,COPA|
^GO Term^Fold Change^Genes^
|mitotic spindle assembly checkpoint signaling|143.29|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|spindle assembly checkpoint signaling|143.29|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|mitotic spindle checkpoint signaling|143.29|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of mitotic sister chromatid segregation|128.96|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of mitotic sister chromatid separation|128.96|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of chromosome segregation|120.90|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of metaphase/anaphase transition of cell cycle|120.90|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of chromosome separation|120.90|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|spindle checkpoint signaling|118.44|KNTC1,TRIP13,BUB3,ZW10,BUB1B,MAD1L1|
|negative regulation of sister chromatid segregation|110.54|KNTC1,TRIP13,BUB3,ZW10,BUB1B,MAD1L1|
|negative regulation of mitotic metaphase/anaphase transition|110.54|KNTC1,TRIP13,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of nuclear division|98.26|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1|
|regulation of mitotic sister chromatid segregation|73.00|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|regulation of chromosome separation|53.73|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|negative regulation of chromosome organization|52.28|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1|
|mitotic cell cycle checkpoint signaling|36.54|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,TRIAP1|
|cell cycle checkpoint signaling|32.09|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,TRIAP1|
|negative regulation of cell cycle process|21.18|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,RBM14,TRIAP1|
|negative regulation of organelle organization|18.82|KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,RBM14,MTOR,TRIAP1|