====== Apcin 50 to 100μM on day4 R04 exp176 ======
==== Mechanism of Action ====
Inhibits substrate interactions with APC/C-Cdc20
  * **<color #00a2e8>Class / Subclass 1:</color>** Proteostasis / E3 ubiquitin ligase inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Cell Cycle / Mitotic Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Apcin%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 300815-04-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/2830389|2830389]]
    * **<color #00a2e8>IUPAC:</color>** %%2-(2-methyl-5-nitroimidazol-1-yl)ethyl N-[2,2,2-trichloro-1-(pyrimidin-2-ylamino)ethyl]carbamate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C13H14Cl3N7O4/c1-8-19-7-9(23(25)26)22(8)5-6-27-12(24)21-10(13(14,15)16)20-11-17-3-2-4-18-11/h2-4,7,10H,5-6H2,1H3,(H,21,24)(H,17,18,20)
    * **<color #00a2e8>INCHI Key:</color>** ZEXHXVOGJFGTRX-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 438.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=NC=C(N1CCOC(=O)NC(C(Cl)(Cl)Cl)NC2=NC=CC=N2)[N+](=O)[O-]
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C13H14Cl3N7O4
{{:chemogenomics:structures:chem-0110.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Boston Biochem
    * **<color #00a2e8>Catalog #:</color>** I-444
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C13H14Cl3N7O4 438.02456; found 438.02545
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Apcin
    * **<color #00a2e8>Min:</color>** -13.2600; **<color #00a2e8>Max:</color>** 16.4769
{{:chemogenomics:dose_response:dr_183.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 04
    * **<color #00a2e8>Dose</color>**: 50-100µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.8
    * **<color #00a2e8>Numbers of reads</color>**: 17845743


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|14/1|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Apcin_50-100day4uM_Round-4_exp176.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Apcin 50 to 100μM on day4 R04 exp176'>
{{:chemogenomics:cranks_plots:exp176.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:U:UBE2S|UBE2S]]|-4.33|<0.01|
|[[:human_genes:O:OTUB1|OTUB1]]|-4.24|<0.01|
|[[:human_genes:A:ANAPC7|ANAPC7]]|-4.11|<0.01|
|[[:human_genes:K:KIF20A|KIF20A]]|-4.10|<0.01|
|[[:human_genes:U:UBE2C|UBE2C]]|-3.52|<0.01|
|[[:human_genes:C:CCNB1|CCNB1]]|-3.39|<0.01|
|[[:human_genes:A:ANAPC13|ANAPC13]]|-3.27|<0.01|
|[[:human_genes:S:SCAP|SCAP]]|-2.76|<0.01|
|[[:human_genes:K:KCTD5|KCTD5]]|-2.63|0.02|
|[[:human_genes:B:BEX5|BEX5]]|-2.62|0.02|
|[[:human_genes:S:SEPSECS|SEPSECS]]|-2.60|0.04|
|[[:human_genes:P:PIK3R4|PIK3R4]]|-2.50|0.03|
|[[:human_genes:B:BRD1|BRD1]]|-2.44|0.04|
|[[:human_genes:D:DNAJC8|DNAJC8]]|-2.40|0.05|
|[[:human_genes:P:PMM2|PMM2]]|-2.36|0.06|
|[[:human_genes:S:SLC5A6|SLC5A6]]|-2.34|0.06|
|[[:human_genes:I:IPO13|IPO13]]|-2.29|0.12|
|[[:human_genes:K:KIAA1432|KIAA1432]]|-2.27|0.08|
|[[:human_genes:A:ANAPC15|ANAPC15]]|-2.27|0.21|
|[[:human_genes:D:DAXX|DAXX]]|-2.22|0.16|
|[[:human_genes:E:ESPL1|ESPL1]]|-2.22|0.58|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.20|0.24|
|[[:human_genes:C:CKS2|CKS2]]|-2.20|0.11|
|[[:human_genes:N:NPRL2|NPRL2]]|-2.20|0.11|
|[[:human_genes:P:PGGT1B|PGGT1B]]|-2.17|0.12|
|[[:human_genes:A:ADNP|ADNP]]|-2.17|0.12|
|[[:human_genes:M:MEDAG|MEDAG]]|-2.16|0.12|
|[[:human_genes:D:DPH1|DPH1]]|-2.13|0.13|
|[[:human_genes:M:MNS1|MNS1]]|-2.08|0.23|
|[[:human_genes:E:EIF4A1|EIF4A1]]|-2.05|0.19|
|[[:human_genes:E:ERCC6L2|ERCC6L2]]|1.95|0.49|
|[[:human_genes:P:POLR3G|POLR3G]]|1.96|0.51|
|[[:human_genes:P:PLXNB1|PLXNB1]]|1.97|0.49|
|[[:human_genes:T:TTYH1|TTYH1]]|1.99|0.51|
|[[:human_genes:G:GPR101|GPR101]]|2.01|0.49|
|[[:human_genes:R:RANBP2|RANBP2]]|2.02|0.49|
|[[:human_genes:R:RSL1D1|RSL1D1]]|2.03|0.49|
|[[:human_genes:H:HOXA7|HOXA7]]|2.04|0.40|
|[[:human_genes:R:ROBO4|ROBO4]]|2.04|0.49|
|[[:human_genes:C:C9orf170|C9orf170]]|2.09|0.49|
|[[:human_genes:S:STAT4|STAT4]]|2.10|0.32|
|[[:human_genes:A:ANKRD23|ANKRD23]]|2.16|0.40|
|[[:human_genes:H:HTRA2|HTRA2]]|2.18|0.26|
|[[:human_genes:P:POLD3|POLD3]]|2.22|0.71|
|[[:human_genes:S:SYT14|SYT14]]|2.23|0.22|
|[[:human_genes:R:RPL30|RPL30]]|2.25|0.73|
|[[:human_genes:C:CSAD|CSAD]]|2.26|0.21|
|[[:human_genes:U:UTP18|UTP18]]|2.26|0.29|
|[[:human_genes:H:HTR1B|HTR1B]]|2.29|0.27|
|[[:human_genes:A:AQR|AQR]]|2.30|0.59|
|[[:human_genes:N:NPIPA8|NPIPA8]]|2.34|0.15|
|[[:human_genes:K:KPNB1|KPNB1]]|2.35|0.57|
|[[:human_genes:N:NPIPA7|NPIPA7]]|2.35|0.15|
|[[:human_genes:T:TUBGCP2|TUBGCP2]]|2.37|0.15|
|[[:human_genes:P:PHB2|PHB2]]|2.40|0.56|
|[[:human_genes:P:PALM|PALM]]|2.45|0.15|
|[[:human_genes:C:CACNA1A|CACNA1A]]|2.48|0.15|
|[[:human_genes:P:POLA2|POLA2]]|2.68|0.53|
|[[:human_genes:K:KRT78|KRT78]]|2.74|0.13|
|[[:human_genes:P:PAQR6|PAQR6]]|3.19|0.02|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp187|proTAME 5μM R04 exp187]]|0.095|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|0.094|<button type="primary" size="small" modal="2">View</button>|
</modal>


<modal id="1" size="lg" title="Apcin 50 to 100μM on day4 R04 exp176  vs  proTAME 5μM R04 exp187">
{{:chemogenomics:comparison_plots:exp176_vs_exp187.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Apcin 25μM plus proTAME 2μM R04 exp177  vs  Apcin 50 to 100μM on day4 R04 exp176">
{{:chemogenomics:comparison_plots:exp176_vs_exp177.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|anaphase-promoting complex|147.98|UBE2S,ANAPC7,UBE2C,ANAPC13,ANAPC15|
|anaphase-promoting complex-dependent catabolic process|147.98|UBE2S,ANAPC7,UBE2C,ANAPC13,ANAPC15|
|positive regulation of metaphase/anaphase transition of cell cycle|126.84|ANAPC7,UBE2C,ESPL1|
|positive regulation of mitotic sister chromatid separation|104.46|ANAPC7,UBE2C,ESPL1|
|protein K11-linked ubiquitination|84.56|UBE2S,ANAPC7,UBE2C,ANAPC13|
|nuclear ubiquitin ligase complex|68.83|UBE2S,ANAPC7,UBE2C,ANAPC13,ANAPC15|
|regulation of mitotic sister chromatid separation|51.03|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of chromosome separation|40.54|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of mitotic metaphase/anaphase transition|33.25|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of metaphase/anaphase transition of cell cycle|32.17|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of sister chromatid segregation|28.73|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of mitotic nuclear division|25.30|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|regulation of chromosome segregation|23.49|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|cullin-RING ubiquitin ligase complex|23.02|UBE2S,ANAPC7,UBE2C,ANAPC13,KCTD5,ANAPC15,CKS2|
|regulation of nuclear division|20.55|ANAPC7,UBE2C,CCNB1,ANAPC15,ESPL1|
|ubiquitin ligase complex|13.32|UBE2S,ANAPC7,UBE2C,ANAPC13,KCTD5,ANAPC15,CKS2|
|cell division|10.53|UBE2S,ANAPC7,KIF20A,UBE2C,CCNB1,ANAPC13,ANAPC15,ESPL1,CKS2|
|transferase complex|7.86|UBE2S,ANAPC7,UBE2C,CCNB1,ANAPC13,KCTD5,PIK3R4,BRD1,ANAPC15,CKS2,PGGT1B|
|intracellular protein-containing complex|6.76|UBE2S,ANAPC7,UBE2C,ANAPC13,KCTD5,BRD1,RIC1,ANAPC15,CKS2,PGGT1B|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>