====== Nocodazole 0.2μM R00 exp24 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Nocodazole%%
    * **<color #00a2e8>Synonyms:</color>** Oncodazole; R17934
    * **<color #00a2e8>CAS #:</color>** 31430-18-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/4122|4122]]
    * **<color #00a2e8>IUPAC:</color>** %%methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
    * **<color #00a2e8>INCHI Key:</color>** KYRVNWMVYQXFEU-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 301.32
    * **<color #00a2e8>Canonical SMILES:</color>** COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C14H11N3O3S
{{:chemogenomics:structures:chem-0013.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** M1404
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Nocodazole
    * **<color #00a2e8>Min:</color>** -9.8754; **<color #00a2e8>Max:</color>** 86.3481
{{:chemogenomics:dose_response:dr_270.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0313    |
| IC30  |0.0403    |
| IC40  |0.0496    |
| IC50  |0.0601    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 200nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.2
    * **<color #00a2e8>Numbers of reads</color>**: 11500839


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/139|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Nocodazole_0.2uM_Round-0_exp24.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Nocodazole 0.2μM R00 exp24'>
{{:chemogenomics:cranks_plots:exp24.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:POLA1|POLA1]]|-3.81|0.10|
|[[:human_genes:G:GOLGA6L1|GOLGA6L1]]|-3.34|0.10|
|[[:human_genes:A:ATP6V0C|ATP6V0C]]|-3.08|0.29|
|[[:human_genes:A:ATP6V1A|ATP6V1A]]|-2.97|0.05|
|[[:human_genes:I:INCENP|INCENP]]|-2.91|0.05|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.87|0.06|
|[[:human_genes:V:VPS28|VPS28]]|-2.86|0.06|
|[[:human_genes:S:SYNCRIP|SYNCRIP]]|-2.74|0.09|
|[[:human_genes:T:TINF2|TINF2]]|-2.58|0.10|
|[[:human_genes:T:TSC2|TSC2]]|-2.58|0.06|
|[[:human_genes:X:XAB2|XAB2]]|-2.55|0.25|
|[[:human_genes:D:DDX3X|DDX3X]]|-2.55|0.06|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-2.52|0.10|
|[[:human_genes:P:PSMB4|PSMB4]]|-2.42|0.16|
|[[:human_genes:A:ATP6V1D|ATP6V1D]]|-2.41|0.10|
|[[:human_genes:C:COPA|COPA]]|-2.37|0.33|
|[[:human_genes:B:BAG2|BAG2]]|-2.37|0.10|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.34|0.10|
|[[:human_genes:T:TRAPPC11|TRAPPC11]]|-2.34|0.10|
|[[:human_genes:R:RPS11|RPS11]]|-2.29|0.38|
|[[:human_genes:P:PCGF1|PCGF1]]|-2.29|0.10|
|[[:human_genes:L:LRRC16A|LRRC16A]]|-2.27|0.10|
|[[:human_genes:V:VPS51|VPS51]]|-2.26|0.16|
|[[:human_genes:N:NRTN|NRTN]]|-2.26|0.10|
|[[:human_genes:E:EMC7|EMC7]]|-2.25|0.16|
|[[:human_genes:S:SOX4|SOX4]]|-2.21|0.25|
|[[:human_genes:N:NCAPD3|NCAPD3]]|-2.21|0.12|
|[[:human_genes:A:AATF|AATF]]|-2.19|0.33|
|[[:human_genes:M:MTCH2|MTCH2]]|-2.19|0.13|
|[[:human_genes:A:AURKB|AURKB]]|-2.18|0.40|
|[[:human_genes:H:HOXA3|HOXA3]]|3.28|<0.01|
|[[:human_genes:P:PLEKHA8|PLEKHA8]]|3.29|<0.01|
|[[:human_genes:I:IGF2BP3|IGF2BP3]]|3.30|<0.01|
|[[:human_genes:H:HOXA13|HOXA13]]|3.30|<0.01|
|[[:human_genes:C:CPVL|CPVL]]|3.39|<0.01|
|[[:human_genes:W:WIPI2|WIPI2]]|3.41|<0.01|
|[[:human_genes:U:USP42|USP42]]|3.47|<0.01|
|[[:human_genes:C:CCZ1B|CCZ1B]]|3.48|<0.01|
|[[:human_genes:T:TRIL|TRIL]]|3.50|<0.01|
|[[:human_genes:E:EVX1|EVX1]]|3.53|<0.01|
|[[:human_genes:C:CYTH3|CYTH3]]|3.58|<0.01|
|[[:human_genes:R:RNF216|RNF216]]|3.61|<0.01|
|[[:human_genes:I:IGFBP1|IGFBP1]]|3.63|<0.01|
|[[:human_genes:A:AMZ1|AMZ1]]|3.79|<0.01|
|[[:human_genes:C:CCZ1|CCZ1]]|3.83|<0.01|
|[[:human_genes:I:IQCE|IQCE]]|3.84|<0.01|
|[[:human_genes:Z:ZWILCH|ZWILCH]]|4.08|<0.01|
|[[:human_genes:S:SDK1|SDK1]]|4.25|<0.01|
|[[:human_genes:A:ADCYAP1R1|ADCYAP1R1]]|4.25|<0.01|
|[[:human_genes:M:MAD1L1|MAD1L1]]|5.10|<0.01|
|[[:human_genes:M:MAD2L1BP|MAD2L1BP]]|5.21|<0.01|
|[[:human_genes:M:MAD2L1|MAD2L1]]|5.62|<0.01|
|[[:human_genes:B:BUB1B|BUB1B]]|5.81|<0.01|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|5.82|<0.01|
|[[:human_genes:C:CDC20|CDC20]]|6.47|<0.01|
|[[:human_genes:R:RBM14|RBM14]]|6.58|<0.01|
|[[:human_genes:B:BUB3|BUB3]]|6.90|<0.01|
|[[:human_genes:T:TRIP13|TRIP13]]|7.81|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|8.29|<0.01|
|[[:human_genes:Z:ZW10|ZW10]]|8.37|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.387|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|0.311|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.245|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.245|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|0.212|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|0.06|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|0.053|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|0.311|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.229|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|0.206|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp17|DABN 20μM R00 exp17]]|0.111|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.085|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|0.063|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|0.055|<button type="primary" size="small" modal="14">View</button>|
</modal>


<modal id="1" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Rotenone 2μM R00 exp33">
{{:chemogenomics:comparison_plots:exp24_vs_exp33.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  SB743921 0.001μM R05 exp244">
{{:chemogenomics:comparison_plots:exp24_vs_exp244.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Vinblastine 0.015μM R05 exp208">
{{:chemogenomics:comparison_plots:exp208_vs_exp24.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  R-DABN 8μM R03 exp111">
{{:chemogenomics:comparison_plots:exp111_vs_exp24.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp24_vs_exp64.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp24.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Pimelic-diphenylamide-106 5μM R00 exp28">
{{:chemogenomics:comparison_plots:exp24_vs_exp28.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp94.png?nolink |}}
</modal>

<modal id="9" size="lg" title="DABN racemic mixture R03 exp93  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp93.png?nolink |}}
</modal>

<modal id="10" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp256.png?nolink |}}
</modal>

<modal id="11" size="lg" title="DABN 20μM R00 exp17  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp17_vs_exp24.png?nolink |}}
</modal>

<modal id="12" size="lg" title="BI-6727 0.04μM R03 exp98  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp98.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Etoposide 1μM R00 exp19  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp19_vs_exp24.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Bortezomib 0.05μM R00 exp7  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp7.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|Golgi lumen acidification|263.21|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D|
|vacuolar proton-transporting V-type ATPase, V0 domain|197.40|ATP6V0C,ATP6V0B,ATP6V0A2|
|ATPase-coupled ion transmembrane transporter activity|128.74|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|proton-transporting ATPase activity, rotational mechanism|128.74|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|ATPase activity, coupled to transmembrane movement of ions, rotational mechanism|128.74|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|vacuolar proton-transporting V-type ATPase complex|123.38|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|lysosomal lumen acidification|112.80|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D|
|proton-transporting V-type ATPase complex|109.67|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|regulation of lysosomal lumen pH|84.60|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D|
|vacuolar acidification|71.78|ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|proton-transporting two-sector ATPase complex|65.80|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|intracellular pH reduction|58.06|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|ATPase-coupled monoatomic cation transmembrane transporter activity|58.06|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|regulation of intracellular pH|34.04|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|endosome organization|33.65|ATP6V0C,ATP6V1A,ATP6V0B,VPS28,ATP6V1D|
|regulation of cellular pH|32.19|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|ATPase-coupled transmembrane transporter activity|31.17|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|regulation of pH|29.03|ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D|
|regulation of macroautophagy|23.07|ATP6V0C,ATP6V1A,ATP6V0B,TSC2,ATP6V0A2,ATP6V1D|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitotic spindle checkpoint signaling|177.66|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|spindle assembly checkpoint signaling|177.66|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|mitotic spindle assembly checkpoint signaling|177.66|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of mitotic sister chromatid separation|160.47|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of mitotic sister chromatid segregation|160.47|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|spindle checkpoint signaling|152.48|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD1L1,ZWILCH|
|negative regulation of chromosome separation|150.75|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of metaphase/anaphase transition of cell cycle|150.75|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of chromosome segregation|150.75|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of mitotic metaphase/anaphase transition|142.64|ZW10,KNTC1,TRIP13,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|negative regulation of sister chromatid segregation|142.64|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD1L1,ZWILCH|
|negative regulation of nuclear division|108.14|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH|
|regulation of mitotic sister chromatid segregation|93.86|ZW10,KNTC1,TRIP13,BUB3,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH|
|regulation of mitotic sister chromatid separation|85.77|ZW10,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH|
|regulation of chromosome separation|75.72|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH|
|regulation of mitotic metaphase/anaphase transition|62.10|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH|
|regulation of metaphase/anaphase transition of cell cycle|60.08|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH|
|regulation of mitotic nuclear division|51.97|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH,HOXA13|
|regulation of nuclear division|42.22|ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH,HOXA13|
</modal>