====== S-trityl-L-cysteine 0.5μM R05 exp243 ======
==== Mechanism of Action ====
Inhibits Eg5 kinesin, blocks centrosome separation and bipolar spindle formation, arrests cells with monoastral spindles
  * **<color #00a2e8>Class / Subclass 1:</color>** Organelle Function / Cytoskeletal Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Cell Cycle / Microtubule Poison



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%S-Trityl-L-cysteine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 2799-07-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/76044|76044]]
    * **<color #00a2e8>IUPAC:</color>** %%(2R)-2-amino-3-tritylsulfanylpropanoic acid%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C22H21NO2S/c23-20(21(24)25)16-26-22(17-10-4-1-5-11-17,18-12-6-2-7-13-18)19-14-8-3-9-15-19/h1-15,20H,16,23H2,(H,24,25)/t20-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** DLMYFMLKORXJPO-FQEVSTJZSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 363.5
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SCC(C(=O)O)N
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SC[C@@H](C(=O)O)N
    * **<color #00a2e8>Molecular Formula:</color>** C22H21NO2S
{{:chemogenomics:structures:chem-0148.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** FOCUS Biomolecules
    * **<color #00a2e8>Catalog #:</color>** 10-1042
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C22H21NO2S 386.11852; found 386.11917
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** STLC
    * **<color #00a2e8>Min:</color>** -14.0321; **<color #00a2e8>Max:</color>** 86.9900
{{:chemogenomics:dose_response:dr_277.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.3654    |
| IC30  |0.4628    |
| IC40  |0.5618    |
| IC50  |0.6711    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 05
    * **<color #00a2e8>Dose</color>**: 500nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.9
    * **<color #00a2e8>Numbers of reads</color>**: 21702335


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|16/1|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/S-Trityl-L-cysteine_0.5uM_Round-5_exp243.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='S-trityl-L-cysteine 0.5μM R05 exp243'>
{{:chemogenomics:cranks_plots:exp243.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:K:KIF18B|KIF18B]]|-5.37|<0.01|
|[[:human_genes:K:KIF15|KIF15]]|-4.43|<0.01|
|[[:human_genes:M:MAPRE1|MAPRE1]]|-3.76|<0.01|
|[[:human_genes:K:KLF16|KLF16]]|-3.53|<0.01|
|[[:human_genes:R:ROCK2|ROCK2]]|-3.19|<0.01|
|[[:human_genes:D:DLGAP5|DLGAP5]]|-3.10|<0.01|
|[[:human_genes:M:MSI2|MSI2]]|-2.86|<0.01|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.75|0.02|
|[[:human_genes:A:ASUN|ASUN]]|-2.64|0.03|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.60|0.04|
|[[:human_genes:S:SPEN|SPEN]]|-2.59|0.02|
|[[:human_genes:T:TIAL1|TIAL1]]|-2.59|0.02|
|[[:human_genes:K:KATNA1|KATNA1]]|-2.59|0.02|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.52|0.20|
|[[:human_genes:T:TUBA1B|TUBA1B]]|-2.48|0.11|
|[[:human_genes:Y:YRDC|YRDC]]|-2.47|0.16|
|[[:human_genes:C:CCDC69|CCDC69]]|-2.45|0.04|
|[[:human_genes:D:DPH1|DPH1]]|-2.40|0.04|
|[[:human_genes:S:STMN1|STMN1]]|-2.39|0.05|
|[[:human_genes:B:BICD2|BICD2]]|-2.38|0.08|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.33|0.16|
|[[:human_genes:T:TRIM72|TRIM72]]|-2.31|0.16|
|[[:human_genes:A:ANKRD17|ANKRD17]]|-2.31|0.07|
|[[:human_genes:C:CHD8|CHD8]]|-2.30|0.11|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.28|0.07|
|[[:human_genes:C:C7orf26|C7orf26]]|-2.26|0.08|
|[[:human_genes:A:ATP2B1|ATP2B1]]|-2.25|0.13|
|[[:human_genes:S:SOX4|SOX4]]|-2.23|0.18|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.23|0.18|
|[[:human_genes:Z:ZNF259|ZNF259]]|-2.20|0.15|
|[[:human_genes:S:SKI|SKI]]|1.93|0.52|
|[[:human_genes:F:FBXO39|FBXO39]]|1.94|0.56|
|[[:human_genes:S:SCAF1|SCAF1]]|1.96|0.77|
|[[:human_genes:P:POLD3|POLD3]]|1.96|0.69|
|[[:human_genes:U:UBALD2|UBALD2]]|2.02|0.52|
|[[:human_genes:H:HCLS1|HCLS1]]|2.02|0.56|
|[[:human_genes:D:DYNC1LI1|DYNC1LI1]]|2.02|0.52|
|[[:human_genes:P:PPP1R14D|PPP1R14D]]|2.06|0.38|
|[[:human_genes:S:STX4|STX4]]|2.07|0.38|
|[[:human_genes:N:NDC80|NDC80]]|2.07|0.70|
|[[:human_genes:Z:ZNF830|ZNF830]]|2.08|0.47|
|[[:human_genes:P:PLK4|PLK4]]|2.09|0.56|
|[[:human_genes:S:SCAPER|SCAPER]]|2.09|0.38|
|[[:human_genes:C:C12orf43|C12orf43]]|2.09|0.38|
|[[:human_genes:R:RBP5|RBP5]]|2.11|0.43|
|[[:human_genes:A:ASPM|ASPM]]|2.12|0.43|
|[[:human_genes:Z:ZNF678|ZNF678]]|2.13|0.43|
|[[:human_genes:R:RUNX3|RUNX3]]|2.13|0.43|
|[[:human_genes:C:CLASP1|CLASP1]]|2.15|0.35|
|[[:human_genes:C:CKAP2|CKAP2]]|2.18|0.33|
|[[:human_genes:E:EIF2B4|EIF2B4]]|2.18|0.56|
|[[:human_genes:R:RSC1A1|RSC1A1]]|2.21|0.38|
|[[:human_genes:R:RFC1|RFC1]]|2.29|0.56|
|[[:human_genes:P:PPP2R5D|PPP2R5D]]|2.36|0.25|
|[[:human_genes:B:BAZ2A|BAZ2A]]|2.38|0.27|
|[[:human_genes:C:CALM2|CALM2]]|2.49|0.25|
|[[:human_genes:F:FARSA|FARSA]]|2.53|0.27|
|[[:human_genes:M:MCL1|MCL1]]|2.78|0.11|
|[[:human_genes:C:COPB2|COPB2]]|2.99|0.27|
|[[:human_genes:N:NDE1|NDE1]]|3.27|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.079|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|0.062|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|0.058|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|0.051|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.121|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|0.102|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|0.083|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|0.083|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.079|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|0.076|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.075|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.075|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.058|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|0.058|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|0.057|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|0.056|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.055|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|0.054|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|0.053|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|0.051|<button type="primary" size="small" modal="20">View</button>|
</modal>


<modal id="1" size="lg" title="S-trityl-L-cysteine 0.5μM R05 exp243  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp190_vs_exp243.png?nolink |}}
</modal>

<modal id="2" size="lg" title="S-trityl-L-cysteine 0.5μM R05 exp243  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp243.png?nolink |}}
</modal>

<modal id="3" size="lg" title="S-trityl-L-cysteine 0.5μM R05 exp243  vs  Vinblastine 0.002μM R01 exp60">
{{:chemogenomics:comparison_plots:exp243_vs_exp60.png?nolink |}}
</modal>

<modal id="4" size="lg" title="S-trityl-L-cysteine 0.5μM R05 exp243  vs  Taxol 0.004μM R03 exp103">
{{:chemogenomics:comparison_plots:exp103_vs_exp243.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp179_vs_exp243.png?nolink |}}
</modal>

<modal id="6" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp310.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp228_vs_exp243.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Colchicine 0.009μM R05 exp215  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp215_vs_exp243.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Ixabepilone 0.004 to 0.005μM on day4 R04 exp184  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp184_vs_exp243.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Cabazitaxel 0.001μM R05 exp223  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp223_vs_exp243.png?nolink |}}
</modal>

<modal id="11" size="lg" title="BML-284 0.09μM R08 exp460  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp460.png?nolink |}}
</modal>

<modal id="12" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp311.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Mdivi-1 15μM R05 exp217  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp217_vs_exp243.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Docosahexaenoic-acid 50μM R07 exp356  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp356.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Epothilone-B 0.0015μM R05 exp231  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp231_vs_exp243.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Docetaxel 0.001 to 0.002μM on day4 R04 exp159  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp159_vs_exp243.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Quercetin 20μM R08 exp517  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp517.png?nolink |}}
</modal>

<modal id="18" size="lg" title="BI-D1870 2μM R04 exp162  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp162_vs_exp243.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp5.png?nolink |}}
</modal>

<modal id="20" size="lg" title="GSK461364A 0.005μM R03 exp125  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp125_vs_exp243.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|centrosome localization|63.49|DLGAP5,INTS13,BICD2|
|microtubule organizing center localization|63.49|DLGAP5,INTS13,BICD2|
|spindle midzone|52.60|KIF18B,MAPRE1,CCDC69|
|spindle organization|17.84|MAPRE1,DLGAP5,INTS13,CCDC69,STMN1|
|microtubule binding|13.90|KIF18B,KIF15,MAPRE1,DLGAP5,KATNA1,CCDC69|
|tubulin binding|11.74|KIF18B,KIF15,MAPRE1,DLGAP5,KATNA1,CCDC69,STMN1|
|microtubule cytoskeleton organization|11.39|KIF18B,MAPRE1,ROCK2,DLGAP5,INTS13,KATNA1,TUBA1B,CCDC69,STMN1,BICD2|
|cell division|9.70|KIF18B,MAPRE1,ROCK2,INTS13,TIAL1,KATNA1,TUBA1B,STMN1|
|microtubule-based process|8.65|KIF18B,KIF15,MAPRE1,ROCK2,DLGAP5,INTS13,KATNA1,TUBA1B,CCDC69,STMN1,BICD2|
|mitotic cell cycle|8.16|KIF18B,KIF15,MAPRE1,ROCK2,DLGAP5,INTS13,TUBA1B,STMN1|
|cytoskeletal protein binding|5.68|KIF18B,KIF15,MAPRE1,ROCK2,DLGAP5,KATNA1,CCDC69,STMN1,BICD2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|spindle localization|36.82|NDE1,CLASP1,ASPM,NDC80|
|centrosome|8.08|NDE1,CALM2,CKAP2,CLASP1,ASPM,PLK4,NDC80,DYNC1LI1,SKI|
|microtubule organizing center|6.17|NDE1,CALM2,CKAP2,CLASP1,ASPM,PLK4,NDC80,DYNC1LI1,SKI|
</modal>