====== HMS-I1 10μM R06 exp256 ======
==== Mechanism of Action ====
Inhibits HDAC
  * **<color #00a2e8>Class / Subclass 1:</color>** Uncharacterized Mechanism / Chemical Screen Hit




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%2-(2,3-Dihydro-1,4-benzodioxin-6-yl)-6-methylimidazo[1,2-a]pyridine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 702636-92-8
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/2813456|2813456]]
    * **<color #00a2e8>IUPAC:</color>** %%2-(2,3-dihydro-1,4-benzodioxin-6-yl)-6-methylimidazo[1,2-a]pyridine%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C16H14N2O2/c1-11-2-5-16-17-13(10-18(16)9-11)12-3-4-14-15(8-12)20-7-6-19-14/h2-5,8-10H,6-7H2,1H3
    * **<color #00a2e8>INCHI Key:</color>** ZNAWJWFMKKSTJM-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 266.29
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=CN2C=C(N=C2C=C1)C3=CC4=C(C=C3)OCCO4
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C16H14N2O2
{{:chemogenomics:structures:chem-0026.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Maybridge
    * **<color #00a2e8>Catalog #:</color>** HTS 07134
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C16H14N2O2 267.1128; found 267.11196
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** EMI1
    * **<color #00a2e8>Min:</color>** 1.1936; **<color #00a2e8>Max:</color>** 97.3773
{{:chemogenomics:dose_response:dr_79.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.5341    |
| IC30  |0.9549    |
| IC40  |1.5374    |
| IC50  |2.3801    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 06
    * **<color #00a2e8>Dose</color>**: 10µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.6
    * **<color #00a2e8>Numbers of reads</color>**: 9286629


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/94|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/HMS-I1_10uM_Round-6_exp256.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='HMS-I1 10μM R06 exp256'>
{{:chemogenomics:cranks_plots:exp256.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:R:RPS19|RPS19]]|-2.71|0.46|
|[[:human_genes:C:CUL4B|CUL4B]]|-2.66|0.38|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.51|0.43|
|[[:human_genes:E:EXOSC6|EXOSC6]]|-2.48|0.54|
|[[:human_genes:L:LIG4|LIG4]]|-2.41|0.43|
|[[:human_genes:N:NAPA|NAPA]]|-2.39|0.51|
|[[:human_genes:A:ADRM1|ADRM1]]|-2.35|0.43|
|[[:human_genes:C:CHAF1A|CHAF1A]]|-2.33|0.50|
|[[:human_genes:N:NPC2|NPC2]]|-2.29|0.43|
|[[:human_genes:P:PSTK|PSTK]]|-2.22|0.46|
|[[:human_genes:P:PSMC2|PSMC2]]|-2.21|0.51|
|[[:human_genes:A:ACACA|ACACA]]|-2.20|0.46|
|[[:human_genes:S:SMNDC1|SMNDC1]]|-2.19|0.50|
|[[:human_genes:C:CFAP45|CFAP45]]|-2.16|0.65|
|[[:human_genes:U:USP17L2|USP17L2]]|-2.15|0.48|
|[[:human_genes:G:GDF9|GDF9]]|-2.14|0.46|
|[[:human_genes:G:GREM2|GREM2]]|-2.11|0.50|
|[[:human_genes:M:MIEF1|MIEF1]]|-2.11|0.46|
|[[:human_genes:F:FAM179B|FAM179B]]|-2.10|0.50|
|[[:human_genes:U:UPF2|UPF2]]|-2.07|0.65|
|[[:human_genes:P:PKHD1|PKHD1]]|-2.06|0.46|
|[[:human_genes:O:OR5H6|OR5H6]]|-2.06|0.46|
|[[:human_genes:S:SMG7|SMG7]]|-2.00|0.54|
|[[:human_genes:P:POLR1B|POLR1B]]|-2.00|0.67|
|[[:human_genes:U:UBXN1|UBXN1]]|-1.98|0.61|
|[[:human_genes:C:CDK12|CDK12]]|-1.98|0.54|
|[[:human_genes:B:BACH2|BACH2]]|-1.98|0.51|
|[[:human_genes:D:DPYD|DPYD]]|-1.97|0.50|
|[[:human_genes:P:POTEH|POTEH]]|-1.97|0.50|
|[[:human_genes:B:BRMS1|BRMS1]]|-1.97|0.54|
|[[:human_genes:A:AMZ1|AMZ1]]|2.82|<0.01|
|[[:human_genes:P:PPP5C|PPP5C]]|2.83|<0.01|
|[[:human_genes:A:ADCYAP1R1|ADCYAP1R1]]|2.84|<0.01|
|[[:human_genes:M:MAD2L1BP|MAD2L1BP]]|2.84|<0.01|
|[[:human_genes:O:OSBPL3|OSBPL3]]|2.84|<0.01|
|[[:human_genes:M:MPLKIP|MPLKIP]]|2.86|<0.01|
|[[:human_genes:N:NFE2L3|NFE2L3]]|2.89|<0.01|
|[[:human_genes:A:AGMO|AGMO]]|2.99|0.00|
|[[:human_genes:R:RADIL|RADIL]]|3.00|<0.01|
|[[:human_genes:I:INMT|INMT]]|3.00|0.00|
|[[:human_genes:I:IGFBP1|IGFBP1]]|3.00|0.00|
|[[:human_genes:M:MAPKAP1|MAPKAP1]]|3.06|<0.01|
|[[:human_genes:M:MEOX2|MEOX2]]|3.09|0.00|
|[[:human_genes:P:PGAM2|PGAM2]]|3.23|0.00|
|[[:human_genes:C:CDC20|CDC20]]|3.28|<0.01|
|[[:human_genes:T:TMED4|TMED4]]|3.42|0.00|
|[[:human_genes:Z:ZWILCH|ZWILCH]]|3.64|0.00|
|[[:human_genes:E:ELAVL1|ELAVL1]]|3.65|0.00|
|[[:human_genes:C:CDCA2|CDCA2]]|3.69|0.00|
|[[:human_genes:L:LRRC72|LRRC72]]|3.75|0.00|
|[[:human_genes:M:MAD2L1|MAD2L1]]|4.08|0.00|
|[[:human_genes:F:FNTB|FNTB]]|4.64|0.00|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|5.50|0.00|
|[[:human_genes:B:BUB3|BUB3]]|5.54|0.00|
|[[:human_genes:M:MAD1L1|MAD1L1]]|5.84|0.00|
|[[:human_genes:B:BUB1B|BUB1B]]|6.06|0.00|
|[[:human_genes:R:RBM14|RBM14]]|6.07|0.00|
|[[:human_genes:Z:ZW10|ZW10]]|6.12|0.00|
|[[:human_genes:T:TRIP13|TRIP13]]|6.17|0.00|
|[[:human_genes:K:KNTC1|KNTC1]]|6.64|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|0.271|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|0.216|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.211|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|0.206|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.189|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.189|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|0.184|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|0.065|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.246|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.089|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|0.059|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp17|DABN 20μM R00 exp17]]|0.056|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|0.055|<button type="primary" size="small" modal="13">View</button>|
</modal>


<modal id="1" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp256_vs_exp94.png?nolink |}}
</modal>

<modal id="2" size="lg" title="HMS-I1 10μM R06 exp256  vs  SB743921 0.001μM R05 exp244">
{{:chemogenomics:comparison_plots:exp244_vs_exp256.png?nolink |}}
</modal>

<modal id="3" size="lg" title="HMS-I1 10μM R06 exp256  vs  Rotenone 2μM R00 exp33">
{{:chemogenomics:comparison_plots:exp256_vs_exp33.png?nolink |}}
</modal>

<modal id="4" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp256.png?nolink |}}
</modal>

<modal id="5" size="lg" title="HMS-I1 10μM R06 exp256  vs  Vinblastine 0.015μM R05 exp208">
{{:chemogenomics:comparison_plots:exp208_vs_exp256.png?nolink |}}
</modal>

<modal id="6" size="lg" title="HMS-I1 10μM R06 exp256  vs  R-DABN 8μM R03 exp111">
{{:chemogenomics:comparison_plots:exp111_vs_exp256.png?nolink |}}
</modal>

<modal id="7" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp256_vs_exp64.png?nolink |}}
</modal>

<modal id="8" size="lg" title="HMS-I1 10μM R06 exp256  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp256.png?nolink |}}
</modal>

<modal id="9" size="lg" title="DABN racemic mixture R03 exp93  vs  HMS-I1 10μM R06 exp256">
{{:chemogenomics:comparison_plots:exp256_vs_exp93.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BI-6727 0.04μM R03 exp98  vs  HMS-I1 10μM R06 exp256">
{{:chemogenomics:comparison_plots:exp256_vs_exp98.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Centrinone 0.5μM R07 exp343  vs  HMS-I1 10μM R06 exp256">
{{:chemogenomics:comparison_plots:exp256_vs_exp343.png?nolink |}}
</modal>

<modal id="12" size="lg" title="DABN 20μM R00 exp17  vs  HMS-I1 10μM R06 exp256">
{{:chemogenomics:comparison_plots:exp17_vs_exp256.png?nolink |}}
</modal>

<modal id="13" size="lg" title="BI-2536 0.02μM R03 exp96  vs  HMS-I1 10μM R06 exp256">
{{:chemogenomics:comparison_plots:exp256_vs_exp96.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|spindle assembly checkpoint signaling|177.56|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|mitotic spindle checkpoint signaling|177.56|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|mitotic spindle assembly checkpoint signaling|177.56|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|negative regulation of mitotic sister chromatid segregation|160.37|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|negative regulation of mitotic sister chromatid separation|160.37|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|spindle checkpoint signaling|152.39|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,ZWILCH,CDC20|
|negative regulation of metaphase/anaphase transition of cell cycle|150.65|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|negative regulation of chromosome separation|150.65|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|negative regulation of chromosome segregation|150.65|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|negative regulation of sister chromatid segregation|142.55|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,ZWILCH,CDC20|
|negative regulation of mitotic metaphase/anaphase transition|142.55|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,MAD2L1,ZWILCH,CDC20|
|negative regulation of nuclear division|108.08|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20|
|regulation of mitotic sister chromatid segregation|93.80|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,MAD2L1BP|
|regulation of mitotic sister chromatid separation|85.72|TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20,MAD2L1BP|
|regulation of chromosome separation|75.67|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20,MAD2L1BP|
|regulation of mitotic metaphase/anaphase transition|62.07|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20,MAD2L1BP|
|regulation of metaphase/anaphase transition of cell cycle|60.04|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,ZWILCH,CDC20,MAD2L1BP|
|regulation of mitotic nuclear division|51.94|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,CDCA2,ZWILCH,CDC20,MAD2L1BP|
|regulation of chromosome segregation|48.23|KNTC1,TRIP13,ZW10,BUB1B,MAD1L1,BUB3,MAD2L1,CDCA2,ZWILCH,CDC20,MAD2L1BP|
</modal>