====== Oligomycin-A 20μM R00 exp26 ====== ==== Mechanism of Action ==== Inhibits F0 proton channel of mitochondrial ATP synthase, blocks electron flow through ETC * **Class / Subclass 1:** Organelle Function / Mitochondrial Inhibitor ==== Technical Notes ==== * **PubChem Name:** %%Oligomycin A%% * **Synonyms:** MCH 32 * **CAS #:** 579-13-5 * **PubChem CID:** [[https://pubchem.ncbi.nlm.nih.gov/compound/5281899|5281899]] * **IUPAC:** %%(1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'-[(2S)-2-hydroxypropyl]-5',6,8,10,12,14,16,28,29-nonamethylspiro[2,26-dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2'-oxane]-3,9,13-trione%% * **INCHI Name:** InChI=1S/C45H74O11/c1-12-34-17-15-13-14-16-27(4)42(51)44(11,53)43(52)32(9)40(50)31(8)39(49)30(7)38(48)26(3)18-21-37(47)54-41-29(6)35(20-19-34)55-45(33(41)10)23-22-25(2)36(56-45)24-28(5)46/h13-15,17-18,21,25-36,38,40-42,46,48,50-51,53H,12,16,19-20,22-24H2,1-11H3/b14-13+,17-15+,21-18+/t25-,26-,27+,28+,29+,30-,31-,32-,33-,34-,35-,36-,38+,40+,41+,42-,44+,45-/m1/s1 * **INCHI Key:** MNULEGDCPYONBU-WMBHJXFZSA-N * **Molecular Weight:** 791.1 * **Canonical SMILES:** CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C * **Isomeric SMILES:** CC[C@H]\\1CC[C@@H]2[C@@H]([C@@H]([C@H]([C@@]3(O2)CC[C@H]([C@H](O3)C[C@H](C)O)C)C)OC(=O)/C=C/[C@H]([C@@H]([C@H](C(=O)[C@H]([C@@H]([C@H](C(=O)[C@@]([C@@H]([C@H](C/C=C/C=C1)C)O)(C)O)C)O)C)C)O)C)C * **Molecular Formula:** C45H74O11 {{:chemogenomics:structures:chem-0014.svg?nolink}} * **Supplier Name:** Tocris Bioscience * **Catalog #:** 4110 * **Lot #:** 6A/200926 * **HRMS (ESI-TOF) m/z:** (M+H)+ Calcd for C45H74O11 791.53039; found 791.53025 Dose response curve not available. ==== Screen Summary ==== * **Round**: 00 * **Dose**: 20µM * **Days of incubation**: 8 * **Doublings**: 0.3 * **Numbers of reads**: 9869667 ==== Screen Results ==== ^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^ |3/0|[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Oligomycin_A_20uM_Round-0_exp26.txt|Scores]]|||||| {{:chemogenomics:cranks_plots:exp26.png?nolink}} ^Gene^CRANKS Score^FDR^ |[[:human_genes:R:RAB3GAP2|RAB3GAP2]]|-4.27|<0.01| |[[:human_genes:A:ATP6AP1|ATP6AP1]]|-3.24|0.22| |[[:human_genes:U:UBE2Q2L|UBE2Q2L]]|-3.02|0.20| |[[:human_genes:T:TBC1D20|TBC1D20]]|-2.90|0.04| |[[:human_genes:R:RAB18|RAB18]]|-2.83|0.02| |[[:human_genes:D:DONSON|DONSON]]|-2.77|0.23| |[[:human_genes:O:OSBP|OSBP]]|-2.75|0.20| |[[:human_genes:O:OR10G3|OR10G3]]|-2.71|0.08| |[[:human_genes:P:POLA1|POLA1]]|-2.67|0.40| |[[:human_genes:P:PSMA3|PSMA3]]|-2.65|0.21| |[[:human_genes:F:FLVCR1|FLVCR1]]|-2.60|0.07| |[[:human_genes:B:BNIP1|BNIP1]]|-2.59|0.06| |[[:human_genes:C:COX7A2L|COX7A2L]]|-2.54|0.06| |[[:human_genes:P:PCYT1A|PCYT1A]]|-2.51|0.06| |[[:human_genes:A:ACOT8|ACOT8]]|-2.49|0.06| |[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-2.39|0.13| |[[:human_genes:P:POLA2|POLA2]]|-2.36|0.31| |[[:human_genes:P:PI4KB|PI4KB]]|-2.35|0.09| |[[:human_genes:L:LRP3|LRP3]]|-2.35|0.09| |[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.33|0.20| |[[:human_genes:P:PCDHGC5|PCDHGC5]]|-2.31|0.15| |[[:human_genes:A:AGPS|AGPS]]|-2.31|0.15| |[[:human_genes:H:HSD17B4|HSD17B4]]|-2.29|0.11| |[[:human_genes:C:COPE|COPE]]|-2.29|0.34| |[[:human_genes:G:GOLGA6L1|GOLGA6L1]]|-2.29|0.40| |[[:human_genes:R:RNF103-CHMP3|RNF103-CHMP3]]|-2.25|0.17| |[[:human_genes:P:PSAP|PSAP]]|-2.25|0.17| |[[:human_genes:T:TMEM138|TMEM138]]|-2.23|0.14| |[[:human_genes:C:COPA|COPA]]|-2.23|0.36| |[[:human_genes:F:FAM231C|FAM231C]]|-2.23|0.21| |[[:human_genes:N:NAA25|NAA25]]|1.95|0.66| |[[:human_genes:M:MRPL47|MRPL47]]|1.95|0.42| |[[:human_genes:C:CCNC|CCNC]]|1.97|0.39| |[[:human_genes:G:GABARAP|GABARAP]]|1.97|0.39| |[[:human_genes:M:MMP9|MMP9]]|1.97|0.44| |[[:human_genes:P:POLR3H|POLR3H]]|1.98|0.39| |[[:human_genes:C:C9orf92|C9orf92]]|2.02|0.35| |[[:human_genes:S:ST20|ST20]]|2.03|0.76| |[[:human_genes:R:RABGGTB|RABGGTB]]|2.05|0.33| |[[:human_genes:E:EIF3F|EIF3F]]|2.06|0.33| |[[:human_genes:T:TRAPPC9|TRAPPC9]]|2.06|0.33| |[[:human_genes:M:MTRNR2L10|MTRNR2L10]]|2.06|0.45| |[[:human_genes:E:EIF3I|EIF3I]]|2.06|0.45| |[[:human_genes:D:DKC1|DKC1]]|2.07|0.66| |[[:human_genes:C:CRCP|CRCP]]|2.08|0.33| |[[:human_genes:G:GFER|GFER]]|2.10|0.44| |[[:human_genes:C:CAB39|CAB39]]|2.11|0.33| |[[:human_genes:A:ANKRD30A|ANKRD30A]]|2.11|0.33| |[[:human_genes:W:WDR48|WDR48]]|2.18|0.33| |[[:human_genes:S:SEC31A|SEC31A]]|2.21|0.23| |[[:human_genes:H:HHLA3|HHLA3]]|2.24|0.22| |[[:human_genes:T:TXNDC17|TXNDC17]]|2.27|0.22| |[[:human_genes:T:TXNRD1|TXNRD1]]|2.30|0.34| |[[:human_genes:S:SMIM15|SMIM15]]|2.31|0.22| |[[:human_genes:K:KARS|KARS]]|2.42|0.22| |[[:human_genes:H:HLTF|HLTF]]|2.44|0.18| |[[:human_genes:A:ALG5|ALG5]]|2.45|0.18| |[[:human_genes:P:PRMT5|PRMT5]]|2.46|0.56| |[[:human_genes:U:UTP14C|UTP14C]]|2.52|0.18| |[[:human_genes:N:NARS|NARS]]|2.56|0.22| ^Screen^Correlation^Plot^ |[[:results:exp34|Rotenone 20μM R00 exp34]]|0.072|| |[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|0.062|| |[[:results:exp17|DABN 20μM R00 exp17]]|0.092|| |[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|0.072|| |[[:results:exp19|Etoposide 1μM R00 exp19]]|0.062|| |[[:results:exp8|Brefeldin A 0.02μM R00 exp8]]|0.054|| |[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.052|| |[[:results:exp469|CFI-400945 25μM R08 exp469]]|0.052|| {{:chemogenomics:comparison_plots:exp26_vs_exp34.png?nolink |}} {{:chemogenomics:comparison_plots:exp26_vs_exp28.png?nolink |}} {{:chemogenomics:comparison_plots:exp17_vs_exp26.png?nolink |}} {{:chemogenomics:comparison_plots:exp26_vs_exp7.png?nolink |}} {{:chemogenomics:comparison_plots:exp19_vs_exp26.png?nolink |}} {{:chemogenomics:comparison_plots:exp26_vs_exp8.png?nolink |}} {{:chemogenomics:comparison_plots:exp199_vs_exp26.png?nolink |}} {{:chemogenomics:comparison_plots:exp26_vs_exp469.png?nolink |}} No GO term hits below threshold. (FDR <0.05) No GO term hits below threshold. (FDR <0.05)