====== 2-Methoxyestradiol 0.55μM R07 exp310 ======
==== Mechanism of Action ====
Endogenous estradiol metabolite, weak agonist for estrogen receptor, agonist of GPER1, microtubule destabilizer
  * **<color #00a2e8>Class / Subclass 1:</color>** Metabolism / Metabolite
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / GPCR Agonist
  * **<color #00a2e8>Class / Subclass 3:</color>** Cell Cycle / Microtubule Poison


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%2-Methoxyestradiol%%
    * **<color #00a2e8>Synonyms:</color>** 2-ME2; NSC-659853
    * **<color #00a2e8>CAS #:</color>** 362-07-2
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/66414|66414]]
    * **<color #00a2e8>IUPAC:</color>** %%(8R,9S,13S,14S,17S)-2-methoxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C19H26O3/c1-19-8-7-12-13(15(19)5-6-18(19)21)4-3-11-9-16(20)17(22-2)10-14(11)12/h9-10,12-13,15,18,20-21H,3-8H2,1-2H3/t12-,13+,15-,18-,19-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** CQOQDQWUFQDJMK-SSTWWWIQSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 302.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC12CCC3C(C1CCC2O)CCC4=CC(=C(C=C34)OC)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=CC(=C(C=C34)OC)O
    * **<color #00a2e8>Molecular Formula:</color>** C19H26O3
{{:chemogenomics:structures:chem-0021.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Toronto Research Chemicals
    * **<color #00a2e8>Catalog #:</color>** M262625
    * **<color #00a2e8>Lot #:</color>** 1-GNM-86-4
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C19H26O3 303.19547; found 303.19491
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Methoxy_b-Estradiol
    * **<color #00a2e8>Min:</color>** -4.8666; **<color #00a2e8>Max:</color>** 98.0088
{{:chemogenomics:dose_response:dr_390.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.1847    |
| IC30  |0.2756    |
| IC40  |0.3897    |
| IC50  |0.5415    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 550nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.5
    * **<color #00a2e8>Numbers of reads</color>**: 24035096


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|4/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/2-Methoxyestradiol_0.55uM_Round-7_exp310.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='2-Methoxyestradiol 0.55μM R07 exp310'>
{{:chemogenomics:cranks_plots:exp310.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SEPHS1|SEPHS1]]|-3.86|<0.01|
|[[:human_genes:A:ASPM|ASPM]]|-3.09|<0.01|
|[[:human_genes:B:BRD1|BRD1]]|-3.00|<0.01|
|[[:human_genes:S:SUGT1|SUGT1]]|-2.87|0.01|
|[[:human_genes:S:SART3|SART3]]|-2.79|0.23|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.54|0.06|
|[[:human_genes:M:MSI2|MSI2]]|-2.50|0.12|
|[[:human_genes:K:KNTC1|KNTC1]]|-2.48|0.12|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.48|0.12|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.47|0.54|
|[[:human_genes:Z:ZNF708|ZNF708]]|-2.38|0.16|
|[[:human_genes:Z:ZNF718|ZNF718]]|-2.33|0.42|
|[[:human_genes:Z:ZWILCH|ZWILCH]]|-2.23|0.16|
|[[:human_genes:C:CNOT2|CNOT2]]|-2.23|0.16|
|[[:human_genes:Z:ZBTB10|ZBTB10]]|-2.22|0.16|
|[[:human_genes:T:TSC1|TSC1]]|-2.17|0.28|
|[[:human_genes:D:DLGAP5|DLGAP5]]|-2.13|0.23|
|[[:human_genes:S:STAG2|STAG2]]|-2.12|0.23|
|[[:human_genes:T:TACC3|TACC3]]|-2.11|0.23|
|[[:human_genes:B:BUB3|BUB3]]|-2.09|0.49|
|[[:human_genes:H:HMGB2|HMGB2]]|-2.09|0.24|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.08|0.38|
|[[:human_genes:T:TRIP13|TRIP13]]|-2.08|0.38|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.07|0.43|
|[[:human_genes:U:UBE2N|UBE2N]]|-2.07|0.25|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.06|0.43|
|[[:human_genes:T:TMED10|TMED10]]|-2.04|0.42|
|[[:human_genes:Q:QDPR|QDPR]]|-2.03|0.29|
|[[:human_genes:G:G2E3|G2E3]]|-2.01|0.42|
|[[:human_genes:S:SAP130|SAP130]]|-2.01|0.42|
|[[:human_genes:P:PLRG1|PLRG1]]|1.90|0.57|
|[[:human_genes:W:WEE1|WEE1]]|1.90|0.69|
|[[:human_genes:N:NUDCD1|NUDCD1]]|1.91|0.57|
|[[:human_genes:N:NBPF1|NBPF1]]|1.91|0.57|
|[[:human_genes:A:ATP1A1|ATP1A1]]|1.92|0.57|
|[[:human_genes:R:RUNDC1|RUNDC1]]|1.92|0.57|
|[[:human_genes:P:PSMB2|PSMB2]]|1.93|0.58|
|[[:human_genes:T:TRIM52|TRIM52]]|1.95|0.71|
|[[:human_genes:M:MEX3C|MEX3C]]|1.95|0.58|
|[[:human_genes:F:FBXO5|FBXO5]]|1.95|0.57|
|[[:human_genes:P:PSMD2|PSMD2]]|1.99|0.57|
|[[:human_genes:M:MARCO|MARCO]]|2.00|0.57|
|[[:human_genes:Z:ZNF710|ZNF710]]|2.00|0.57|
|[[:human_genes:T:TIPRL|TIPRL]]|2.01|0.57|
|[[:human_genes:L:LIG1|LIG1]]|2.02|0.83|
|[[:human_genes:S:SMIM15|SMIM15]]|2.03|0.57|
|[[:human_genes:N:NUMA1|NUMA1]]|2.05|0.57|
|[[:human_genes:P:PEX16|PEX16]]|2.08|0.57|
|[[:human_genes:C:CCNF|CCNF]]|2.08|0.57|
|[[:human_genes:P:PRRC1|PRRC1]]|2.11|0.57|
|[[:human_genes:K:KCNN4|KCNN4]]|2.12|0.57|
|[[:human_genes:E:EIF2B4|EIF2B4]]|2.12|0.57|
|[[:human_genes:C:CADPS|CADPS]]|2.12|0.57|
|[[:human_genes:W:WDR75|WDR75]]|2.32|0.57|
|[[:human_genes:C:CDC27|CDC27]]|2.34|0.57|
|[[:human_genes:H:HAP1|HAP1]]|2.37|0.48|
|[[:human_genes:C:CMC2|CMC2]]|2.46|0.57|
|[[:human_genes:K:KIF18B|KIF18B]]|2.48|0.14|
|[[:human_genes:S:SCG2|SCG2]]|2.51|0.14|
|[[:human_genes:K:KIF2C|KIF2C]]|2.53|0.14|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|0.126|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.102|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.089|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.089|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.087|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.084|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp59|UMK57 1μM R01 exp59]]|0.074|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.069|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.068|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|0.067|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|0.062|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|0.059|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|0.059|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp46|HMS-I1 1μM R01 exp46]]|0.056|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|0.056|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|0.054|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.187|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.105|<button type="primary" size="small" modal="18">View</button>|
</modal>


<modal id="1" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp310.png?nolink |}}
</modal>

<modal id="2" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp310.png?nolink |}}
</modal>

<modal id="3" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  BML-284 0.09μM R08 exp460">
{{:chemogenomics:comparison_plots:exp310_vs_exp460.png?nolink |}}
</modal>

<modal id="4" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp190_vs_exp310.png?nolink |}}
</modal>

<modal id="5" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Combretastatin A4 0.002 to 0.003μM day4 R04 exp179">
{{:chemogenomics:comparison_plots:exp179_vs_exp310.png?nolink |}}
</modal>

<modal id="6" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Mdivi-1 15μM R05 exp217">
{{:chemogenomics:comparison_plots:exp217_vs_exp310.png?nolink |}}
</modal>

<modal id="7" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  UMK57 1μM R01 exp59">
{{:chemogenomics:comparison_plots:exp310_vs_exp59.png?nolink |}}
</modal>

<modal id="8" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp184_vs_exp310.png?nolink |}}
</modal>

<modal id="9" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Quercetin 20μM R08 exp517">
{{:chemogenomics:comparison_plots:exp310_vs_exp517.png?nolink |}}
</modal>

<modal id="10" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp310.png?nolink |}}
</modal>

<modal id="11" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Cabazitaxel 0.001μM R05 exp223">
{{:chemogenomics:comparison_plots:exp223_vs_exp310.png?nolink |}}
</modal>

<modal id="12" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Vinblastine 0.002μM R01 exp60">
{{:chemogenomics:comparison_plots:exp310_vs_exp60.png?nolink |}}
</modal>

<modal id="13" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Docosahexaenoic-acid 50μM R07 exp356">
{{:chemogenomics:comparison_plots:exp310_vs_exp356.png?nolink |}}
</modal>

<modal id="14" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  HMS-I1 1μM R01 exp46">
{{:chemogenomics:comparison_plots:exp310_vs_exp46.png?nolink |}}
</modal>

<modal id="15" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp310_vs_exp32.png?nolink |}}
</modal>

<modal id="16" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp310.png?nolink |}}
</modal>

<modal id="17" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  2-Methoxyestradiol 0.55μM R07 exp310">
{{:chemogenomics:comparison_plots:exp310_vs_exp311.png?nolink |}}
</modal>

<modal id="18" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  2-Methoxyestradiol 0.55μM R07 exp310">
{{:chemogenomics:comparison_plots:exp310_vs_exp312.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|protein localization to condensed chromosome|159.41|KNTC1,ZWILCH,BUB3|
|kinetochore assembly|147.15|SUGT1,KNTC1,DLGAP5|
|mitotic spindle assembly checkpoint signaling|98.10|KNTC1,ZWILCH,BUB3,TRIP13|
|mitotic spindle checkpoint signaling|98.10|KNTC1,ZWILCH,BUB3,TRIP13|
|spindle checkpoint signaling|94.47|KNTC1,ZWILCH,BUB3,TRIP13|
|spindle assembly checkpoint signaling|94.47|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of mitotic sister chromatid separation|87.95|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of sister chromatid segregation|87.95|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of mitotic sister chromatid segregation|85.02|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of chromosome segregation|82.28|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of chromosome separation|82.28|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of metaphase/anaphase transition of cell cycle|82.28|KNTC1,ZWILCH,BUB3,TRIP13|
|negative regulation of nuclear division|57.97|KNTC1,ZWILCH,BUB3,TRIP13|
|regulation of chromosome separation|44.91|KNTC1,ZWILCH,DLGAP5,BUB3,TRIP13|
|regulation of sister chromatid segregation|37.88|KNTC1,ZWILCH,DLGAP5,TACC3,BUB3,TRIP13|
|regulation of mitotic metaphase/anaphase transition|36.65|KNTC1,ZWILCH,DLGAP5,BUB3,TRIP13|
|regulation of metaphase/anaphase transition of cell cycle|35.43|KNTC1,ZWILCH,DLGAP5,BUB3,TRIP13|
|regulation of mitotic nuclear division|27.72|KNTC1,ZWILCH,DLGAP5,BUB3,TRIP13|
|nuclear chromosome segregation|17.55|ASPM,KNTC1,DLGAP5,STAG2,BUB3,TRIP13|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|cell division|8.75|KIF2C,KIF18B,CDC27,CCNF,NUMA1,LIG1,FBXO5,WEE1|
</modal>