====== Calcium Ionophore 0.4μM R07 exp342 ======
==== Mechanism of Action ====
Calcium ionophore
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Membrane Ionophore



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Calcium ionophore A23187%%
    * **<color #00a2e8>Synonyms:</color>** A-23187; Antibiotic A-23187
    * **<color #00a2e8>CAS #:</color>** 52665-69-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/11957499|11957499]]
    * **<color #00a2e8>IUPAC:</color>** %%5-(methylamino)-2-[[(2S,3R,5R,6S,8R,9R)-3,5,9-trimethyl-2-[(2S)-1-oxo-1-(1H-pyrrol-2-yl)propan-2-yl]-1,7-dioxaspiro[5.5]undecan-8-yl]methyl]-1,3-benzoxazole-4-carboxylic acid%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C29H37N3O6/c1-15-10-11-29(17(3)13-16(2)27(38-29)18(4)26(33)20-7-6-12-31-20)37-22(15)14-23-32-25-21(36-23)9-8-19(30-5)24(25)28(34)35/h6-9,12,15-18,22,27,30-31H,10-11,13-14H2,1-5H3,(H,34,35)/t15-,16-,17-,18-,22-,27+,29+/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** HIYAVKIYRIFSCZ-CYEMHPAKSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 523.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CCC2(C(CC(C(O2)C(C)C(=O)C3=CC=CN3)C)C)OC1CC4=NC5=C(O4)C=CC(=C5C(=O)O)NC
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1CC[C@]2([C@@H](C[C@H]([C@H](O2)[C@H](C)C(=O)C3=CC=CN3)C)C)O[C@@H]1CC4=NC5=C(O4)C=CC(=C5C(=O)O)NC
    * **<color #00a2e8>Molecular Formula:</color>** C29H37N3O6
{{:chemogenomics:structures:chem-0200.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C7522
    * **<color #00a2e8>Lot #:</color>** 037M4005V
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C29H37N3O6 524.27551; found 524.27274
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Ca_Ionophore_A23187
    * **<color #00a2e8>Min:</color>** -7.3147; **<color #00a2e8>Max:</color>** 99.7771
{{:chemogenomics:dose_response:dr_375.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.2509    |
| IC30  |0.3503    |
| IC40  |0.4698    |
| IC50  |0.6220    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 400nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.6
    * **<color #00a2e8>Numbers of reads</color>**: 29297319


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|7/13|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Calcium_Ionophore_A23187_0.4uM_Round-7_exp342.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Calcium Ionophore 0.4μM R07 exp342'>
{{:chemogenomics:cranks_plots:exp342.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GOT1|GOT1]]|-3.34|<0.01|
|[[:human_genes:F:FDFT1|FDFT1]]|-2.85|0.02|
|[[:human_genes:F:FDPS|FDPS]]|-2.79|0.02|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-2.76|0.02|
|[[:human_genes:P:PBRM1|PBRM1]]|-2.74|0.02|
|[[:human_genes:K:KRTAP4-12|KRTAP4-12]]|-2.63|0.03|
|[[:human_genes:H:HSD17B12|HSD17B12]]|-2.55|0.14|
|[[:human_genes:C:COX7A2L|COX7A2L]]|-2.52|0.05|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.46|0.06|
|[[:human_genes:E:EIF5B|EIF5B]]|-2.44|0.57|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.33|0.61|
|[[:human_genes:E:ENO1|ENO1]]|-2.32|0.16|
|[[:human_genes:M:ME2|ME2]]|-2.28|0.14|
|[[:human_genes:P:PLA2G2A|PLA2G2A]]|-2.26|0.14|
|[[:human_genes:M:MOB1A|MOB1A]]|-2.25|0.14|
|[[:human_genes:T:TCF4|TCF4]]|-2.20|0.16|
|[[:human_genes:B:BCL2|BCL2]]|-2.19|0.16|
|[[:human_genes:U:UBE2I|UBE2I]]|-2.16|0.30|
|[[:human_genes:M:MRS2|MRS2]]|-2.15|0.30|
|[[:human_genes:C:CUL5|CUL5]]|-2.12|0.32|
|[[:human_genes:D:DHX33|DHX33]]|-2.11|0.47|
|[[:human_genes:S:SPATS2L|SPATS2L]]|-2.11|0.23|
|[[:human_genes:A:ABCB7|ABCB7]]|-2.09|0.23|
|[[:human_genes:L:LEF1|LEF1]]|-2.09|0.23|
|[[:human_genes:G:GSN|GSN]]|-2.09|0.35|
|[[:human_genes:P:PIAS4|PIAS4]]|-2.04|0.39|
|[[:human_genes:P:PARL|PARL]]|-2.03|0.39|
|[[:human_genes:U:UXS1|UXS1]]|-2.02|0.30|
|[[:human_genes:D:DHX29|DHX29]]|-1.97|0.35|
|[[:human_genes:E:EEFSEC|EEFSEC]]|-1.93|0.39|
|[[:human_genes:N:NDUFA11|NDUFA11]]|2.28|0.09|
|[[:human_genes:C:C16orf80|C16orf80]]|2.30|0.13|
|[[:human_genes:M:MEIS3|MEIS3]]|2.33|0.07|
|[[:human_genes:P:PCYT1A|PCYT1A]]|2.34|0.05|
|[[:human_genes:T:TSFM|TSFM]]|2.34|0.05|
|[[:human_genes:M:MBTPS2|MBTPS2]]|2.36|0.07|
|[[:human_genes:D:DPH1|DPH1]]|2.38|0.05|
|[[:human_genes:E:EBF1|EBF1]]|2.40|0.06|
|[[:human_genes:S:STARD7|STARD7]]|2.40|0.06|
|[[:human_genes:N:NDUFB9|NDUFB9]]|2.41|0.06|
|[[:human_genes:R:RSF1|RSF1]]|2.41|0.09|
|[[:human_genes:N:NDUFV1|NDUFV1]]|2.42|0.09|
|[[:human_genes:S:SLC7A1|SLC7A1]]|2.46|0.06|
|[[:human_genes:N:NDUFA2|NDUFA2]]|2.50|0.05|
|[[:human_genes:Z:ZNF217|ZNF217]]|2.53|0.05|
|[[:human_genes:P:POU2F1|POU2F1]]|2.57|0.06|
|[[:human_genes:E:EIF2AK1|EIF2AK1]]|2.65|0.03|
|[[:human_genes:N:NDUFS5|NDUFS5]]|2.65|0.05|
|[[:human_genes:C:CXorf56|CXorf56]]|2.68|0.03|
|[[:human_genes:Y:YLPM1|YLPM1]]|2.79|0.04|
|[[:human_genes:C:C12orf49|C12orf49]]|2.80|<0.01|
|[[:human_genes:N:NANS|NANS]]|3.04|<0.01|
|[[:human_genes:N:NFU1|NFU1]]|3.12|<0.01|
|[[:human_genes:E:EIF4A1|EIF4A1]]|3.19|<0.01|
|[[:human_genes:N:NDUFB11|NDUFB11]]|3.43|<0.01|
|[[:human_genes:A:AMD1|AMD1]]|3.57|<0.01|
|[[:human_genes:M:MICU1|MICU1]]|3.77|<0.01|
|[[:human_genes:S:SCAP|SCAP]]|4.06|<0.01|
|[[:human_genes:R:RBM26|RBM26]]|4.14|<0.01|
|[[:human_genes:A:ATP2B1|ATP2B1]]|4.53|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|0.095|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.082|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp360|Genistein 15μM R07 exp360]]|0.076|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.069|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|0.068|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.059|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|0.056|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|0.055|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.055|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|0.054|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|0.052|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|0.051|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|0.066|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|0.056|<button type="primary" size="small" modal="14">View</button>|
</modal>


<modal id="1" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp342.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Lead acetate 2000μM R08 exp497">
{{:chemogenomics:comparison_plots:exp342_vs_exp497.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp342_vs_exp360.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp342_vs_exp492.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Lead acetate 2000μM R08 exp498 no dilution day6">
{{:chemogenomics:comparison_plots:exp342_vs_exp498.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Hydroxyurea 100μM R07 exp329">
{{:chemogenomics:comparison_plots:exp329_vs_exp342.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  HMS-I2 10μM R06 exp288">
{{:chemogenomics:comparison_plots:exp288_vs_exp342.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  QNZ 0.01μM R05 exp241">
{{:chemogenomics:comparison_plots:exp241_vs_exp342.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Thapsigargin 0.005μM R07 exp407">
{{:chemogenomics:comparison_plots:exp342_vs_exp407.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Tubastatin-A 2.5μM R07 exp417">
{{:chemogenomics:comparison_plots:exp342_vs_exp417.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307">
{{:chemogenomics:comparison_plots:exp307_vs_exp342.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Pterostilbene 16μM R02 exp78">
{{:chemogenomics:comparison_plots:exp342_vs_exp78.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Artemisinin 50μM R08 exp450  vs  Calcium Ionophore 0.4μM R07 exp342">
{{:chemogenomics:comparison_plots:exp342_vs_exp450.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Atovaquone 15μM R08 exp451  vs  Calcium Ionophore 0.4μM R07 exp342">
{{:chemogenomics:comparison_plots:exp342_vs_exp451.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|NADH dehydrogenase complex|94.35|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|respiratory chain complex I|94.35|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|mitochondrial respiratory chain complex I|92.11|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|NADH dehydrogenase complex assembly|74.39|NDUFB11,NDUFS5,NDUFA2,NDUFB9,NDUFA11,NUBPL,NDUFA1|
|mitochondrial respiratory chain complex I assembly|74.39|NDUFB11,NDUFS5,NDUFA2,NDUFB9,NDUFA11,NUBPL,NDUFA1|
|proton motive force-driven ATP synthesis|64.47|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|proton motive force-driven mitochondrial ATP synthesis|62.56|NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|ATP biosynthetic process|55.26|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|respiratory chain complex|52.28|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|mitochondrial respirasome|51.58|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|purine ribonucleoside triphosphate biosynthetic process|47.76|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|purine nucleoside triphosphate biosynthetic process|47.18|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|respirasome|47.18|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|ribonucleoside triphosphate biosynthetic process|44.98|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|mitochondrial respiratory chain complex assembly|44.46|NDUFB11,NDUFS5,NDUFA2,NDUFB9,NDUFA11,NUBPL,NDUFA1|
|nucleoside triphosphate biosynthetic process|41.15|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|oxidative phosphorylation|39.08|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|oxidoreductase complex|34.23|NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
|inner mitochondrial membrane protein complex|33.24|MICU1,NDUFB11,NDUFS5,NDUFA2,NDUFV1,NDUFB9,NDUFA11,NDUFA1|
</modal>