====== TRAIL 5ng/ml R00 exp35 ======
==== Mechanism of Action ====
TNF-related apoptosis-inducing ligand, TNF superfamily member, induces extrinsic apoptosis
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Other Receptor Agonist




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%N/A%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** N/A
    * **<color #00a2e8>PubChem CID:</color>** N/A
    * **<color #00a2e8>IUPAC:</color>** %%N/A%%
    * **<color #00a2e8>INCHI Name:</color>** N/A
    * **<color #00a2e8>INCHI Key:</color>** N/A
    * **<color #00a2e8>Molecular Weight:</color>** 19.6kDa
    * **<color #00a2e8>Canonical SMILES:</color>** N/A
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE
{{:chemogenomics:structures:chem-0019.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Peprotech
    * **<color #00a2e8>Catalog #:</color>** 310-04
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
Characterization data not available.
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Trail
    * **<color #00a2e8>Min:</color>** -9.0552; **<color #00a2e8>Max:</color>** 7.2598
{{:chemogenomics:dose_response:dr_278.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 5ng/mL
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 2.9
    * **<color #00a2e8>Numbers of reads</color>**: 13057838


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|56/130|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/TRAIL_5_ng_per_mL_Round-0_exp35.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='TRAIL 5ng/ml R00 exp35'>
{{:chemogenomics:cranks_plots:exp35.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:CNOT2|CNOT2]]|-4.63|<0.01|
|[[:human_genes:S:SZT2|SZT2]]|-4.31|<0.01|
|[[:human_genes:Z:ZBTB10|ZBTB10]]|-4.23|<0.01|
|[[:human_genes:A:ARID4B|ARID4B]]|-3.86|<0.01|
|[[:human_genes:X:XIAP|XIAP]]|-3.64|<0.01|
|[[:human_genes:C:C12orf66|C12orf66]]|-3.61|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.60|<0.01|
|[[:human_genes:C:C12orf44|C12orf44]]|-3.33|<0.01|
|[[:human_genes:C:CNOT8|CNOT8]]|-3.29|<0.01|
|[[:human_genes:T:TMEM30A|TMEM30A]]|-3.28|<0.01|
|[[:human_genes:B:BCL11A|BCL11A]]|-3.28|<0.01|
|[[:human_genes:W:WDR20|WDR20]]|-3.27|<0.01|
|[[:human_genes:A:ATG9A|ATG9A]]|-3.25|<0.01|
|[[:human_genes:W:WIPI2|WIPI2]]|-3.17|<0.01|
|[[:human_genes:O:OTUB1|OTUB1]]|-3.14|<0.01|
|[[:human_genes:P:PCBP2|PCBP2]]|-3.03|<0.01|
|[[:human_genes:R:RNF19A|RNF19A]]|-3.01|<0.01|
|[[:human_genes:A:ANKRD52|ANKRD52]]|-3.00|<0.01|
|[[:human_genes:S:SESN2|SESN2]]|-2.99|<0.01|
|[[:human_genes:T:TRAF2|TRAF2]]|-2.98|<0.01|
|[[:human_genes:M:MAPKAP1|MAPKAP1]]|-2.97|<0.01|
|[[:human_genes:R:RNF19B|RNF19B]]|-2.96|<0.01|
|[[:human_genes:D:DEPDC5|DEPDC5]]|-2.85|<0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.82|0.01|
|[[:human_genes:I:ITFG2|ITFG2]]|-2.81|<0.01|
|[[:human_genes:A:ATG14|ATG14]]|-2.79|<0.01|
|[[:human_genes:S:STUB1|STUB1]]|-2.68|<0.01|
|[[:human_genes:S:SNRNP40|SNRNP40]]|-2.64|<0.01|
|[[:human_genes:T:TSC2|TSC2]]|-2.62|<0.01|
|[[:human_genes:M:MEF2D|MEF2D]]|-2.61|0.05|
|[[:human_genes:G:GCN1L1|GCN1L1]]|4.27|<0.01|
|[[:human_genes:B:BTAF1|BTAF1]]|4.43|<0.01|
|[[:human_genes:I:IKZF1|IKZF1]]|4.47|<0.01|
|[[:human_genes:S:SEC31A|SEC31A]]|4.49|<0.01|
|[[:human_genes:E:EIF4A1|EIF4A1]]|4.50|<0.01|
|[[:human_genes:C:CLK3|CLK3]]|4.56|<0.01|
|[[:human_genes:C:CDK6|CDK6]]|4.60|<0.01|
|[[:human_genes:T:TP53|TP53]]|4.63|<0.01|
|[[:human_genes:Z:ZC3HAV1|ZC3HAV1]]|4.65|<0.01|
|[[:human_genes:S:SPOP|SPOP]]|4.65|<0.01|
|[[:human_genes:F:FNIP1|FNIP1]]|4.72|<0.01|
|[[:human_genes:F:FLCN|FLCN]]|4.83|<0.01|
|[[:human_genes:C:CHD7|CHD7]]|4.89|<0.01|
|[[:human_genes:D:DDX3X|DDX3X]]|4.91|<0.01|
|[[:human_genes:H:HNRNPL|HNRNPL]]|5.27|<0.01|
|[[:human_genes:Z:ZNF217|ZNF217]]|5.37|<0.01|
|[[:human_genes:M:MTMR4|MTMR4]]|5.59|<0.01|
|[[:human_genes:U:UBE3A|UBE3A]]|5.78|<0.01|
|[[:human_genes:C:CREB1|CREB1]]|6.03|<0.01|
|[[:human_genes:C:CCNC|CCNC]]|6.50|<0.01|
|[[:human_genes:K:KHDRBS1|KHDRBS1]]|6.50|<0.01|
|[[:human_genes:C:CXorf38|CXorf38]]|7.25|<0.01|
|[[:human_genes:I:IKZF2|IKZF2]]|7.29|<0.01|
|[[:human_genes:F:FBXO11|FBXO11]]|7.72|<0.01|
|[[:human_genes:T:TNFRSF10B|TNFRSF10B]]|7.74|<0.01|
|[[:human_genes:Z:ZBTB7A|ZBTB7A]]|7.85|<0.01|
|[[:human_genes:E:ELAVL1|ELAVL1]]|8.00|<0.01|
|[[:human_genes:C:CASP3|CASP3]]|8.14|<0.01|
|[[:human_genes:F:FADD|FADD]]|8.49|<0.01|
|[[:human_genes:C:CASP8|CASP8]]|8.49|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|0.062|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.054|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.051|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|0.408|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|0.087|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|0.075|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|0.06|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.06|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp211|AICAR 240μM R05 exp211]]|0.06|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.058|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|0.056|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|0.056|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|0.052|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|0.052|<button type="primary" size="small" modal="14">View</button>|
</modal>


<modal id="1" size="lg" title="TRAIL 5ng/ml R00 exp35  vs  Tubacin 1.6μM R07 exp416">
{{:chemogenomics:comparison_plots:exp35_vs_exp416.png?nolink |}}
</modal>

<modal id="2" size="lg" title="TRAIL 5ng/ml R00 exp35  vs  Thapsigargin 0.005μM R07 exp407">
{{:chemogenomics:comparison_plots:exp35_vs_exp407.png?nolink |}}
</modal>

<modal id="3" size="lg" title="TRAIL 5ng/ml R00 exp35  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp35_vs_exp38.png?nolink |}}
</modal>

<modal id="4" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp36.png?nolink |}}
</modal>

<modal id="5" size="lg" title="MLN-4924 0.2μM R00 exp21  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp21_vs_exp35.png?nolink |}}
</modal>

<modal id="6" size="lg" title="3-Bromopyruvate 7μM R04 exp175  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp175_vs_exp35.png?nolink |}}
</modal>

<modal id="7" size="lg" title="LY2090314 0.003μM R08 exp499  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp499.png?nolink |}}
</modal>

<modal id="8" size="lg" title="GSK626616 14μM R08 exp485  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp485.png?nolink |}}
</modal>

<modal id="9" size="lg" title="AICAR 240μM R05 exp211  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp211_vs_exp35.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Quercetin 20μM R08 exp517  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp517.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Bortezomib 0.005μM R00 exp6  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp6.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Aminopterin 0.005μM R07 exp335  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp335_vs_exp35.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Actinomycin-D 0.001μM R00 exp3  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp3_vs_exp35.png?nolink |}}
</modal>

<modal id="14" size="lg" title="LY2090314 0.003μM R08 exp500 no dilution day6  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp500.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|negative regulation of TORC1 signaling|97.06|SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|cellular response to amino acid starvation|70.56|SZT2,SESN2,DEPDC5,NPRL3,ITFG2|
|response to amino acid starvation|66.32|SZT2,SESN2,DEPDC5,NPRL3,ITFG2|
|negative regulation of TOR signaling|60.29|SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|cellular response to glucose starvation|55.27|SZT2,SESN2,ITFG2,ATG14|
|regulation of TORC1 signaling|53.98|SZT2,OTUB1,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|cellular response to starvation|31.21|SZT2,WIPI2,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|regulation of TOR signaling|29.48|SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|cellular response to nutrient levels|25.73|SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|response to starvation|25.39|SZT2,WIPI2,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|cellular response to extracellular stimulus|22.87|SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|cellular response to external stimulus|18.31|SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|vacuolar membrane|12.81|SZT2,TMEM30A,ATG9A,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|response to nutrient levels|12.62|SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|response to extracellular stimulus|11.89|SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
|positive regulation of catabolic process|11.61|CNOT2,CNOT8,RNF19A,SESN2,RNF19B,DEPDC5,NPRL3,STUB1,TSC2|
|lytic vacuole membrane|10.95|SZT2,TMEM30A,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|lysosomal membrane|10.95|SZT2,TMEM30A,SESN2,DEPDC5,NPRL3,ITFG2,TSC2|
|vacuole|8.82|SZT2,TMEM30A,ATG9A,WIPI2,SESN2,RNF19B,DEPDC5,NPRL3,ITFG2,ATG14,TSC2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|death effector domain binding|368.47|CASP8,FADD|
|TRAIL-activated apoptotic signaling pathway|276.35|CASP8,FADD,TNFRSF10B|
|death-inducing signaling complex|207.26|CASP8,FADD,CASP3|
|death receptor binding|82.91|CASP8,FADD,CASP3|
|extrinsic apoptotic signaling pathway via death domain receptors|52.64|CASP8,FADD,TNFRSF10B,DDX3X|
|regulation of TORC1 signaling|25.71|UBE3A,FLCN,FNIP1,LAMTOR2|
|intrinsic apoptotic signaling pathway|17.27|CASP3,TNFRSF10B,DDX3X,FLCN,TP53|
|lymphocyte differentiation|13.62|FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1|
|apoptotic signaling pathway|12.56|CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,TP53|
|leukocyte differentiation|11.93|CASP8,FADD,ZBTB7A,CREB1,CHD7,FNIP1,TP53,CDK6,IKZF1|
|cellular response to external stimulus|11.87|CASP8,FADD,TNFRSF10B,FLCN,FNIP1,TP53,GCN1|
|mononuclear cell differentiation|11.83|FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1|
|mRNA binding|10.63|ELAVL1,KHDRBS1,HNRNPL,DDX3X,TP53,EIF4A1|
|cellular response to environmental stimulus|10.56|CASP8,FADD,CASP3,TNFRSF10B,DDX3X,TP53|
|lymphocyte activation|9.55|CASP8,FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1|
|hemopoiesis|9.11|CASP8,FADD,CASP3,ZBTB7A,CREB1,CHD7,FLCN,FNIP1,TP53,CDK6,IKZF1|
|positive regulation of apoptotic process|9.08|CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,FNIP1,TP53|
|positive regulation of programmed cell death|8.70|CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,FNIP1,TP53|
|leukocyte activation|7.58|CASP8,FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1|
</modal>