====== Dexamethasone 0.006 to 0.015μM on day4 R07 exp352 ======
==== Mechanism of Action ====
Glucocorticoid receptor agonist, anti-inflammatory
  * **<color #00a2e8>Class / Subclass 1:</color>** Metabolism / Hormone Agonist
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / GPCR Agonist



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Dexamethasone%%
    * **<color #00a2e8>Synonyms:</color>** Hexadecadrol; Prednisolone F
    * **<color #00a2e8>CAS #:</color>** 50-02-2
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5743|5743]]
    * **<color #00a2e8>IUPAC:</color>** %%(8S,9R,10S,11S,13S,14S,16R,17R)-9-fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8,11,12,14,15,16-octahydrocyclopenta[a]phenanthren-3-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** UREBDLICKHMUKA-CXSFZGCWSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 392.5
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CC2C3CCC4=CC(=O)C=CC4(C3(C(CC2(C1(C(=O)CO)O)C)O)F)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@@]4([C@]3([C@H](C[C@@]2([C@]1(C(=O)CO)O)C)O)F)C
    * **<color #00a2e8>Molecular Formula:</color>** C22H29FO5
{{:chemogenomics:structures:chem-0208.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** D4902
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C22H29FO5 393.20718; found 393.2068
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Dexamethasone
    * **<color #00a2e8>Min:</color>** -4.7517; **<color #00a2e8>Max:</color>** 71.3612
{{:chemogenomics:dose_response:dr_463.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0111    |
| IC30  |0.0159    |
| IC40  |0.0223    |
| IC50  |0.0178    |
| IC60  |N/A    |
| IC70  |0.2312    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 0.006-0.015µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.2
    * **<color #00a2e8>Numbers of reads</color>**: 14131811


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|13/45|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Dexamethasone_0.006-0.015day4uM_Round-7_exp352.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Dexamethasone 0.006 to 0.015μM on day4 R07 exp352'>
{{:chemogenomics:cranks_plots:exp352.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SPEN|SPEN]]|-3.64|<0.01|
|[[:human_genes:F:FKBP5|FKBP5]]|-3.29|<0.01|
|[[:human_genes:N:NRAS|NRAS]]|-3.23|<0.01|
|[[:human_genes:C:CREBBP|CREBBP]]|-3.07|<0.01|
|[[:human_genes:N:NFKBIA|NFKBIA]]|-2.92|<0.01|
|[[:human_genes:C:CYLD|CYLD]]|-2.86|<0.01|
|[[:human_genes:P:PPP2CA|PPP2CA]]|-2.67|0.04|
|[[:human_genes:E:ERGIC1|ERGIC1]]|-2.60|0.05|
|[[:human_genes:Z:ZNF652|ZNF652]]|-2.57|0.05|
|[[:human_genes:D:DPH1|DPH1]]|-2.54|0.04|
|[[:human_genes:D:DERL2|DERL2]]|-2.52|0.04|
|[[:human_genes:S:SHOC2|SHOC2]]|-2.52|0.05|
|[[:human_genes:L:LEF1|LEF1]]|-2.45|0.05|
|[[:human_genes:T:TRIM33|TRIM33]]|-2.43|0.05|
|[[:human_genes:M:MED16|MED16]]|-2.40|0.13|
|[[:human_genes:A:AFF4|AFF4]]|-2.39|0.05|
|[[:human_genes:A:ABHD5|ABHD5]]|-2.36|0.10|
|[[:human_genes:S:SCAF8|SCAF8]]|-2.35|0.06|
|[[:human_genes:T:TCF4|TCF4]]|-2.29|0.07|
|[[:human_genes:C:CTCF|CTCF]]|-2.27|0.13|
|[[:human_genes:U:UBE4B|UBE4B]]|-2.24|0.10|
|[[:human_genes:T:TMSB4X|TMSB4X]]|-2.22|0.14|
|[[:human_genes:D:DPF2|DPF2]]|-2.21|0.10|
|[[:human_genes:P:PHIP|PHIP]]|-2.20|0.10|
|[[:human_genes:R:RAP1GDS1|RAP1GDS1]]|-2.17|0.12|
|[[:human_genes:G:GPR75-ASB3|GPR75-ASB3]]|-2.16|0.19|
|[[:human_genes:R:REC8|REC8]]|-2.14|0.20|
|[[:human_genes:L:LDB1|LDB1]]|-2.14|0.13|
|[[:human_genes:U:UFSP2|UFSP2]]|-2.13|0.13|
|[[:human_genes:N:NSD1|NSD1]]|-2.12|0.20|
|[[:human_genes:P:POLE2|POLE2]]|2.59|0.14|
|[[:human_genes:S:SNRNP70|SNRNP70]]|2.64|0.01|
|[[:human_genes:N:NF1|NF1]]|2.66|<0.01|
|[[:human_genes:T:TCF3|TCF3]]|2.69|0.02|
|[[:human_genes:K:KPTN|KPTN]]|2.69|<0.01|
|[[:human_genes:P:PPP3R1|PPP3R1]]|2.70|<0.01|
|[[:human_genes:H:HIST1H4E|HIST1H4E]]|2.77|<0.01|
|[[:human_genes:K:KCTD5|KCTD5]]|2.77|<0.01|
|[[:human_genes:C:CCDC117|CCDC117]]|2.83|<0.01|
|[[:human_genes:U:UBE2H|UBE2H]]|2.89|<0.01|
|[[:human_genes:G:GPS2|GPS2]]|2.98|<0.01|
|[[:human_genes:A:ARID3A|ARID3A]]|2.99|<0.01|
|[[:human_genes:E:EIF4B|EIF4B]]|3.10|0.00|
|[[:human_genes:S:SCAP|SCAP]]|3.10|0.00|
|[[:human_genes:Z:ZNF292|ZNF292]]|3.17|0.00|
|[[:human_genes:A:AXIN1|AXIN1]]|3.22|<0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|3.34|<0.01|
|[[:human_genes:D:DDX3X|DDX3X]]|3.39|0.00|
|[[:human_genes:C:C12orf66|C12orf66]]|3.55|0.00|
|[[:human_genes:Y:YPEL5|YPEL5]]|3.64|0.00|
|[[:human_genes:S:SUV39H1|SUV39H1]]|3.75|0.00|
|[[:human_genes:G:GSK3B|GSK3B]]|3.82|0.00|
|[[:human_genes:N:NPRL2|NPRL2]]|3.87|0.00|
|[[:human_genes:N:NANS|NANS]]|4.11|0.00|
|[[:human_genes:A:ACTB|ACTB]]|4.17|0.00|
|[[:human_genes:S:STIP1|STIP1]]|4.54|0.00|
|[[:human_genes:P:PTGES3|PTGES3]]|4.73|0.00|
|[[:human_genes:T:TSC1|TSC1]]|4.82|0.00|
|[[:human_genes:T:TSC2|TSC2]]|7.07|0.00|
|[[:human_genes:N:NR3C1|NR3C1]]|7.72|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|0.228|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.075|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp504|MK2206 4μM R08 exp504]]|0.074|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|0.064|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.063|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|0.062|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|0.061|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|0.061|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.06|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|0.06|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|0.054|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.054|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|0.052|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp467|CAY10603 0.55μM R08 exp467]]|0.052|<button type="primary" size="small" modal="14">View</button>|
</modal>


<modal id="1" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Dexamethasone 0.006μM R07 exp351">
{{:chemogenomics:comparison_plots:exp351_vs_exp352.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  GSK626616 14μM R08 exp485">
{{:chemogenomics:comparison_plots:exp352_vs_exp485.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  MK2206 4μM R08 exp504">
{{:chemogenomics:comparison_plots:exp352_vs_exp504.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307">
{{:chemogenomics:comparison_plots:exp307_vs_exp352.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Thapsigargin 0.005μM R07 exp407">
{{:chemogenomics:comparison_plots:exp352_vs_exp407.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  I-BRD9 4μM R07 exp365">
{{:chemogenomics:comparison_plots:exp352_vs_exp365.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Rapamycin 2μM R07 exp306">
{{:chemogenomics:comparison_plots:exp306_vs_exp352.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Rapamycin 0.001μM R08 exp429">
{{:chemogenomics:comparison_plots:exp352_vs_exp429.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Rapamycin 1μM R00 exp29">
{{:chemogenomics:comparison_plots:exp29_vs_exp352.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Erlotinib 10μM R05 exp216">
{{:chemogenomics:comparison_plots:exp216_vs_exp352.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Momordin-Ic 10μM R08 exp506">
{{:chemogenomics:comparison_plots:exp352_vs_exp506.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp352_vs_exp38.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp352_vs_exp36.png?nolink |}}
</modal>

<modal id="14" size="lg" title="CAY10603 0.55μM R08 exp467  vs  Dexamethasone 0.006 to 0.015μM on day4 R07 exp352">
{{:chemogenomics:comparison_plots:exp352_vs_exp467.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|beta-catenin-TCF complex|153.51|LEF1,TCF4,LDB1|
|transcription factor binding|8.33|SPEN,CREBBP,NFKBIA,LEF1,MED16,TCF4,CTCF,LDB1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|Seh1-associated complex|236.84|NPRL2,NPRL3,DEPDC5|
|negative regulation of TORC1 signaling|94.35|TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|Hsp90 protein binding|69.08|NR3C1,TSC2,TSC1,PTGES3,STIP1|
|cellular response to amino acid starvation|58.79|NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|negative regulation of TOR signaling|58.61|TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|response to amino acid starvation|55.26|NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|regulation of TORC1 signaling|44.98|TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|regulation of TOR signaling|28.66|TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2|
|cellular response to starvation|26.01|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|heat shock protein binding|22.28|NR3C1,TSC2,TSC1,PTGES3,STIP1|
|response to starvation|21.15|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|cellular response to nutrient levels|19.06|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|cellular response to extracellular stimulus|16.94|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|cellular response to external stimulus|13.56|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|negative regulation of intracellular signal transduction|11.85|TSC2,TSC1,NPRL2,GSK3B,DDX3X,NPRL3,GPS2,KPTN,NF1,DEPDC5,ITFG2|
|regulation of autophagy|11.12|TSC2,TSC1,NPRL2,GSK3B,NPRL3,SNRNP70,DEPDC5|
|response to nutrient levels|9.35|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|response to extracellular stimulus|8.81|TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2|
|intracellular protein-containing complex|6.95|TSC1,PTGES3,STIP1,ACTB,GSK3B,YPEL5,AXIN1,EIF4B,KCTD5,PPP3R1,POLE2|
</modal>