====== Wortmannin 5μM R00 exp38 ======
==== Mechanism of Action ====
Inhibits PI3K and PI3K-related enzymes, non-specific covalent inhibitor
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Proteostasis / Autophagy Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Wortmannin%%
    * **<color #00a2e8>Synonyms:</color>** SL-2052; KY-12420
    * **<color #00a2e8>CAS #:</color>** 19545-26-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/312145|312145]]
    * **<color #00a2e8>IUPAC:</color>** %%[(1R,3R,5S,9R,18S)-18-(methoxymethyl)-1,5-dimethyl-6,11,16-trioxo-13,17-dioxapentacyclo[10.6.1.02,10.05,9.015,19]nonadeca-2(10),12(19),14-trien-3-yl] acetate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C23H24O8/c1-10(24)30-13-7-22(2)12(5-6-14(22)25)16-18(13)23(3)15(9-28-4)31-21(27)11-8-29-20(17(11)23)19(16)26/h8,12-13,15H,5-7,9H2,1-4H3/t12-,13+,15+,22-,23-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** QDLHCMPXEPAAMD-QAIWCSMKSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 428.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC(=O)OC1CC2(C(CCC2=O)C3=C1C4(C(OC(=O)C5=COC(=C54)C3=O)COC)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** CC(=O)O[C@@H]1C[C@]2([C@@H](CCC2=O)C3=C1[C@]4([C@H](OC(=O)C5=COC(=C54)C3=O)COC)C)C
    * **<color #00a2e8>Molecular Formula:</color>** C23H24O8
{{:chemogenomics:structures:chem-0020.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** LC Laboratories
    * **<color #00a2e8>Catalog #:</color>** W-2990
    * **<color #00a2e8>Lot #:</color>** SS-107
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C23H24O8 451.13634; found 451.1358
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Wortmannin
    * **<color #00a2e8>Min:</color>** -10.7381; **<color #00a2e8>Max:</color>** 63.3011
{{:chemogenomics:dose_response:dr_280.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |1.6783    |
| IC30  |3.4255    |
| IC40  |6.1479    |
| IC50  |10.5153    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 5µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 3.4
    * **<color #00a2e8>Numbers of reads</color>**: 12718276


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|30/18|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Wortmannin_5uM_Round-0_exp38.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Wortmannin 5μM R00 exp38'>
{{:chemogenomics:cranks_plots:exp38.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GPR107|GPR107]]|-4.60|<0.01|
|[[:human_genes:Q:QRICH1|QRICH1]]|-4.35|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.37|<0.01|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-3.27|<0.01|
|[[:human_genes:D:DCAF15|DCAF15]]|-3.20|<0.01|
|[[:human_genes:T:TRAF2|TRAF2]]|-3.11|<0.01|
|[[:human_genes:R:RSRC2|RSRC2]]|-3.02|0.05|
|[[:human_genes:A:ARL1|ARL1]]|-2.94|<0.01|
|[[:human_genes:H:HSPA4|HSPA4]]|-2.80|0.01|
|[[:human_genes:P:PIK3CA|PIK3CA]]|-2.77|<0.01|
|[[:human_genes:Z:ZBTB10|ZBTB10]]|-2.74|0.02|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.70|0.01|
|[[:human_genes:C:C10orf76|C10orf76]]|-2.67|0.01|
|[[:human_genes:P:PRKAA1|PRKAA1]]|-2.63|0.01|
|[[:human_genes:S:SYK|SYK]]|-2.62|0.01|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-2.59|0.02|
|[[:human_genes:M:MAPKAP1|MAPKAP1]]|-2.58|0.03|
|[[:human_genes:P:PGGT1B|PGGT1B]]|-2.56|0.02|
|[[:human_genes:A:ASB3|ASB3]]|-2.50|0.02|
|[[:human_genes:R:RIF1|RIF1]]|-2.46|0.05|
|[[:human_genes:O:ORC3|ORC3]]|-2.45|0.07|
|[[:human_genes:C:CREBBP|CREBBP]]|-2.44|0.07|
|[[:human_genes:W:WDR20|WDR20]]|-2.41|0.05|
|[[:human_genes:S:SEC23B|SEC23B]]|-2.39|0.05|
|[[:human_genes:A:ACOT7|ACOT7]]|-2.38|0.04|
|[[:human_genes:P:PIK3C3|PIK3C3]]|-2.37|0.06|
|[[:human_genes:F:FURIN|FURIN]]|-2.35|0.06|
|[[:human_genes:N:NME1-NME2|NME1-NME2]]|-2.35|0.05|
|[[:human_genes:T:TNFAIP3|TNFAIP3]]|-2.34|0.05|
|[[:human_genes:H:HDAC1|HDAC1]]|-2.33|0.05|
|[[:human_genes:C:CMYA5|CMYA5]]|2.19|0.32|
|[[:human_genes:S:SPRED2|SPRED2]]|2.24|0.08|
|[[:human_genes:H:HERC4|HERC4]]|2.30|0.06|
|[[:human_genes:P:PTEN|PTEN]]|2.32|0.05|
|[[:human_genes:U:USP36|USP36]]|2.32|0.05|
|[[:human_genes:L:LCMT1|LCMT1]]|2.34|0.05|
|[[:human_genes:T:TNFRSF21|TNFRSF21]]|2.34|0.09|
|[[:human_genes:P:PMAIP1|PMAIP1]]|2.42|0.04|
|[[:human_genes:P:PSMD1|PSMD1]]|2.45|0.39|
|[[:human_genes:L:LZTR1|LZTR1]]|2.48|0.03|
|[[:human_genes:U:UBQLN1|UBQLN1]]|2.49|0.03|
|[[:human_genes:K:KCTD5|KCTD5]]|2.56|0.02|
|[[:human_genes:M:MCL1|MCL1]]|2.57|0.07|
|[[:human_genes:R:RCSD1|RCSD1]]|2.59|0.03|
|[[:human_genes:W:WDR43|WDR43]]|2.63|0.22|
|[[:human_genes:V:VDAC2|VDAC2]]|2.63|0.02|
|[[:human_genes:M:MPC2|MPC2]]|2.63|0.02|
|[[:human_genes:M:MARCH5|MARCH5]]|2.65|0.03|
|[[:human_genes:N:NPIPB3|NPIPB3]]|2.66|0.32|
|[[:human_genes:N:NKTR|NKTR]]|2.66|0.10|
|[[:human_genes:Z:ZDHHC17|ZDHHC17]]|2.72|0.01|
|[[:human_genes:U:UBAP1|UBAP1]]|2.80|0.02|
|[[:human_genes:D:DUSP6|DUSP6]]|2.81|<0.01|
|[[:human_genes:D:DOLPP1|DOLPP1]]|2.83|0.02|
|[[:human_genes:H:HMG20B|HMG20B]]|2.86|<0.01|
|[[:human_genes:S:SMARCC1|SMARCC1]]|2.99|<0.01|
|[[:human_genes:T:TMEM39A|TMEM39A]]|3.11|<0.01|
|[[:human_genes:N:NF1|NF1]]|3.24|<0.01|
|[[:human_genes:B:BAK1|BAK1]]|3.93|<0.01|
|[[:human_genes:T:TXNIP|TXNIP]]|4.16|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|0.091|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|0.07|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp467|CAY10603 0.55μM R08 exp467]]|0.07|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|0.063|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.061|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|0.056|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.056|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.056|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|0.056|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|0.054|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.053|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|0.053|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|0.051|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|0.051|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|0.051|<button type="primary" size="small" modal="15">View</button>|
</modal>


<modal id="1" size="lg" title="Wortmannin 0.5μM R00 exp37  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp37_vs_exp38.png?nolink |}}
</modal>

<modal id="2" size="lg" title="UM0129480 7μM R02 exp85  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp85.png?nolink |}}
</modal>

<modal id="3" size="lg" title="CAY10603 0.55μM R08 exp467  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp467.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Dexamethasone 0.006μM R07 exp351  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp351_vs_exp38.png?nolink |}}
</modal>

<modal id="5" size="lg" title="GSK626616 14μM R08 exp485  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp485.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp15_vs_exp38.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Thapsigargin 0.005μM R07 exp407  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp407.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Lead acetate 2000μM R08 exp497  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp497.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Dorsomorphin 5μM R07 exp357  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp357_vs_exp38.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp352_vs_exp38.png?nolink |}}
</modal>

<modal id="11" size="lg" title="WYE-354 6μM R02 exp90  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp90.png?nolink |}}
</modal>

<modal id="12" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp36_vs_exp38.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp5.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Ibrutinib 10μM R08 exp442  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp442.png?nolink |}}
</modal>

<modal id="15" size="lg" title="TRAIL 5ng/ml R00 exp35  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp35_vs_exp38.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|Bcl-2 family protein complex|184.23|BAK1,MCL1,PMAIP1|
|release of cytochrome c from mitochondria|78.96|BAK1,MCL1,PMAIP1|
|apoptotic mitochondrial changes|39.48|BAK1,VDAC2,MCL1,PMAIP1|
|regulation of apoptotic signaling pathway|10.32|BAK1,NF1,VDAC2,MCL1,UBQLN1,PMAIP1,PTEN|
</modal>