====== Alpha-Amanitin 0.5μM R00 exp5 ======
==== Mechanism of Action ====
Inhibits RNA polymerase II and III, interferes with translocation
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Transcription Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Environmental Stresses / Toxin



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%alpha-Amanitin%%
    * **<color #00a2e8>Synonyms:</color>** α-Amanitin; α-Amatoxin
    * **<color #00a2e8>CAS #:</color>** 23109-05-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/441541|441541]]
    * **<color #00a2e8>IUPAC:</color>** %%2-[(1R,4S,8R,10S,13S,16S,34S)-34-[(2S)-butan-2-yl]-13-[(2R,3R)-3,4-dihydroxybutan-2-yl]-8,22-dihydroxy-2,5,11,14,27,30,33,36,39-nonaoxo-27λ4-thia-3,6,12,15,25,29,32,35,38-nonazapentacyclo[14.12.11.06,10.018,26.019,24]nonatriaconta-18(26),19(24),20,22-tetraen-4-yl]acetamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)/t16-,17-,19+,23-,24-,25-,26-,27-,31-,32-,64?/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** CIORWBWIBBPXCG-JAXJKTSHSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 919
    * **<color #00a2e8>Canonical SMILES:</color>** CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@H](C)[C@H]1C(=O)NCC(=O)N[C@H]2CS(=O)C3=C(C[C@@H](C(=O)NCC(=O)N1)NC(=O)[C@@H](NC(=O)[C@@H]4C[C@H](CN4C(=O)[C@@H](NC2=O)CC(=O)N)O)[C@@H](C)[C@H](CO)O)C5=C(N3)C=C(C=C5)O
    * **<color #00a2e8>Molecular Formula:</color>** C39H54N10O14S
{{:chemogenomics:structures:chem-0003.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** A2263
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C39H54N10O14S 919.36144; found 919.36336
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Alpha-amanitin
    * **<color #00a2e8>Min:</color>** -15.5942; **<color #00a2e8>Max:</color>** 56.4508
{{:chemogenomics:dose_response:dr_255.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |9.6912    |
| IC30  |15.9233    |
| IC40  |23.9227    |
| IC50  |34.7566    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 500nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.4
    * **<color #00a2e8>Numbers of reads</color>**: 10256944


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|7/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Alpha-Amanitin_0.5uM_Round-0_exp5.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Alpha-Amanitin 0.5μM R00 exp5'>
{{:chemogenomics:cranks_plots:exp5.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ADK|ADK]]|-3.42|<0.01|
|[[:human_genes:R:RER1|RER1]]|-3.21|<0.01|
|[[:human_genes:S:SHOC2|SHOC2]]|-3.13|<0.01|
|[[:human_genes:X:XPO5|XPO5]]|-2.92|0.06|
|[[:human_genes:S:SNRPB2|SNRPB2]]|-2.82|0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.75|0.06|
|[[:human_genes:S:SZT2|SZT2]]|-2.72|0.02|
|[[:human_genes:T:TRPM4|TRPM4]]|-2.67|0.05|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.65|0.42|
|[[:human_genes:M:MSI2|MSI2]]|-2.63|0.03|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.55|0.44|
|[[:human_genes:T:TNKS|TNKS]]|-2.48|0.08|
|[[:human_genes:M:MCM9|MCM9]]|-2.45|0.06|
|[[:human_genes:S:SPEN|SPEN]]|-2.41|0.10|
|[[:human_genes:T:TCF4|TCF4]]|-2.40|0.06|
|[[:human_genes:O:ORC3|ORC3]]|-2.37|0.16|
|[[:human_genes:M:MTFMT|MTFMT]]|-2.34|0.13|
|[[:human_genes:E:EPS8L2|EPS8L2]]|-2.31|0.09|
|[[:human_genes:T:TSC2|TSC2]]|-2.26|0.10|
|[[:human_genes:I:ILF3|ILF3]]|-2.22|0.29|
|[[:human_genes:H:HIST1H2BD|HIST1H2BD]]|-2.21|0.18|
|[[:human_genes:P:POU2F1|POU2F1]]|-2.21|0.16|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.20|0.13|
|[[:human_genes:P:PEX6|PEX6]]|-2.18|0.17|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.18|0.13|
|[[:human_genes:C:CPSF3L|CPSF3L]]|-2.17|0.13|
|[[:human_genes:T:TMEM249|TMEM249]]|-2.16|0.14|
|[[:human_genes:G:GEMIN4|GEMIN4]]|-2.14|0.15|
|[[:human_genes:V:VPS37A|VPS37A]]|-2.12|0.15|
|[[:human_genes:O:OR4F4|OR4F4]]|-2.12|0.18|
|[[:human_genes:E:EPB41L2|EPB41L2]]|2.07|0.30|
|[[:human_genes:S:ST20|ST20]]|2.07|0.52|
|[[:human_genes:N:NUP85|NUP85]]|2.08|0.48|
|[[:human_genes:N:NUP153|NUP153]]|2.09|0.39|
|[[:human_genes:A:ARHGEF35|ARHGEF35]]|2.09|0.39|
|[[:human_genes:R:RPL23|RPL23]]|2.12|0.47|
|[[:human_genes:R:RPA1|RPA1]]|2.13|0.40|
|[[:human_genes:R:RPL23A|RPL23A]]|2.14|0.40|
|[[:human_genes:T:TOP2A|TOP2A]]|2.16|0.27|
|[[:human_genes:E:EFCAB2|EFCAB2]]|2.17|0.27|
|[[:human_genes:U:USP27X|USP27X]]|2.20|0.55|
|[[:human_genes:K:KRTAP4-11|KRTAP4-11]]|2.20|0.38|
|[[:human_genes:K:KCTD16|KCTD16]]|2.20|0.30|
|[[:human_genes:M:MBTD1|MBTD1]]|2.23|0.30|
|[[:human_genes:M:MGST2|MGST2]]|2.24|0.27|
|[[:human_genes:P:PSMA4|PSMA4]]|2.24|0.27|
|[[:human_genes:T:TRAPPC2L|TRAPPC2L]]|2.26|0.27|
|[[:human_genes:Z:ZFAND3|ZFAND3]]|2.27|0.27|
|[[:human_genes:U:UBAP1|UBAP1]]|2.29|0.27|
|[[:human_genes:T:TLR10|TLR10]]|2.29|0.27|
|[[:human_genes:O:OR10G3|OR10G3]]|2.31|0.30|
|[[:human_genes:R:RPL7A|RPL7A]]|2.37|0.46|
|[[:human_genes:P:PLK4|PLK4]]|2.39|0.39|
|[[:human_genes:D:DONSON|DONSON]]|2.40|0.46|
|[[:human_genes:N:NUP155|NUP155]]|2.50|0.27|
|[[:human_genes:N:NOP16|NOP16]]|2.74|0.39|
|[[:human_genes:M:MYO1H|MYO1H]]|2.84|0.19|
|[[:human_genes:P:PRPF8|PRPF8]]|3.00|0.30|
|[[:human_genes:T:TFRC|TFRC]]|3.02|0.39|
|[[:human_genes:R:RFC1|RFC1]]|3.77|0.19|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|0.062|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|0.06|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.057|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|0.057|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|0.056|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|0.053|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.053|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.051|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|0.051|<button type="primary" size="small" modal="10">View</button>|
</modal>


<modal id="1" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp31_vs_exp5.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Pimelic-diphenylamide-106 0.5μM R00 exp27">
{{:chemogenomics:comparison_plots:exp27_vs_exp5.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp184_vs_exp5.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  CCCP 0.1μM R00 exp10">
{{:chemogenomics:comparison_plots:exp10_vs_exp5.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Wortmannin 0.5μM R00 exp37">
{{:chemogenomics:comparison_plots:exp37_vs_exp5.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Dynasore 7μM R08 exp436">
{{:chemogenomics:comparison_plots:exp436_vs_exp5.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp32_vs_exp5.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp243_vs_exp5.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp5.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Cycloheximide 0.2μM R00 exp15">
{{:chemogenomics:comparison_plots:exp15_vs_exp5.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>