====== ZLN024 50μM R08 exp538 ======
==== Mechanism of Action ====
Activates AMPK, allosteric
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Activator




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%ZLN024 hydrochloride%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1883548-91-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/91654626|91654626]]
    * **<color #00a2e8>IUPAC:</color>** %%2-[2-(2-bromo-4-methylphenoxy)ethylsulfanyl]pyrimidine;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C13H13BrN2OS.ClH/c1-10-3-4-12(11(14)9-10)17-7-8-18-13-15-5-2-6-16-13;/h2-6,9H,7-8H2,1H3;1H
    * **<color #00a2e8>INCHI Key:</color>** MMQCYIMDROWWFE-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 361.69
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=CC(=C(C=C1)OCCSC2=NC=CC=N2)Br.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C13H14BrClN2OS
{{:chemogenomics:structures:chem-0342.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-16708A
    * **<color #00a2e8>Lot #:</color>** 14644
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C13H13BrN2OS 325.00047; found 325.00098
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** ZLN024
    * **<color #00a2e8>Min:</color>** -4.5126; **<color #00a2e8>Max:</color>** 42.3824
{{:chemogenomics:dose_response:dr_598.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 50µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.5
    * **<color #00a2e8>Numbers of reads</color>**: 15051894


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|10/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/ZLN024_50uM_Round-8_exp538.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='ZLN024 50μM R08 exp538'>
{{:chemogenomics:cranks_plots:exp538.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SPTLC1|SPTLC1]]|-4.04|<0.01|
|[[:human_genes:K:KDSR|KDSR]]|-3.59|<0.01|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-3.24|<0.01|
|[[:human_genes:C:CHKA|CHKA]]|-3.24|<0.01|
|[[:human_genes:S:SPTLC2|SPTLC2]]|-3.18|<0.01|
|[[:human_genes:M:MS4A13|MS4A13]]|-2.96|0.42|
|[[:human_genes:A:ACSL1|ACSL1]]|-2.79|<0.01|
|[[:human_genes:C:C22orf23|C22orf23]]|-2.79|0.05|
|[[:human_genes:S:SPTSSA|SPTSSA]]|-2.77|0.01|
|[[:human_genes:S:SCAP|SCAP]]|-2.74|0.01|
|[[:human_genes:T:TCF4|TCF4]]|-2.47|0.04|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-2.44|0.09|
|[[:human_genes:C:CDIPT|CDIPT]]|-2.44|0.27|
|[[:human_genes:A:ACACA|ACACA]]|-2.40|0.10|
|[[:human_genes:D:DNAJC3|DNAJC3]]|-2.40|0.06|
|[[:human_genes:P:PDSS1|PDSS1]]|-2.40|0.10|
|[[:human_genes:U:UACA|UACA]]|-2.33|0.09|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-2.29|0.09|
|[[:human_genes:S:SUPV3L1|SUPV3L1]]|-2.25|0.27|
|[[:human_genes:S:SMIM11A|SMIM11A]]|-2.25|0.62|
|[[:human_genes:M:MBTPS1|MBTPS1]]|-2.24|0.27|
|[[:human_genes:R:RARS2|RARS2]]|-2.21|0.12|
|[[:human_genes:T:TRPM8|TRPM8]]|-2.18|0.22|
|[[:human_genes:B:BPIFA1|BPIFA1]]|-2.12|0.19|
|[[:human_genes:D:DNAJC7|DNAJC7]]|-2.10|0.21|
|[[:human_genes:P:PSKH1|PSKH1]]|-2.10|0.27|
|[[:human_genes:C:CALML4|CALML4]]|-2.08|0.35|
|[[:human_genes:Z:ZNF329|ZNF329]]|-2.07|0.22|
|[[:human_genes:C:CEPT1|CEPT1]]|-2.07|0.22|
|[[:human_genes:A:AEBP2|AEBP2]]|-2.06|0.22|
|[[:human_genes:H:HNRNPC|HNRNPC]]|1.93|0.46|
|[[:human_genes:C:C18orf32|C18orf32]]|1.93|0.79|
|[[:human_genes:P:PDS5A|PDS5A]]|1.93|0.46|
|[[:human_genes:T:TBC1D32|TBC1D32]]|1.94|0.46|
|[[:human_genes:T:TUBA1C|TUBA1C]]|1.95|0.56|
|[[:human_genes:C:CALR3|CALR3]]|1.96|0.45|
|[[:human_genes:R:RBM42|RBM42]]|1.98|0.49|
|[[:human_genes:H:HAT1|HAT1]]|1.99|0.42|
|[[:human_genes:P:PPP1R16B|PPP1R16B]]|2.00|0.42|
|[[:human_genes:P:PCGF1|PCGF1]]|2.02|0.40|
|[[:human_genes:M:MTA2|MTA2]]|2.02|0.40|
|[[:human_genes:F:FDPS|FDPS]]|2.02|0.47|
|[[:human_genes:K:KIAA1586|KIAA1586]]|2.03|0.40|
|[[:human_genes:G:GIMAP7|GIMAP7]]|2.04|0.40|
|[[:human_genes:C:COG6|COG6]]|2.12|0.36|
|[[:human_genes:C:CD47|CD47]]|2.16|0.40|
|[[:human_genes:S:SNRNP200|SNRNP200]]|2.16|0.47|
|[[:human_genes:S:SMC3|SMC3]]|2.17|0.52|
|[[:human_genes:Z:ZBTB18|ZBTB18]]|2.19|0.35|
|[[:human_genes:D:DDX3X|DDX3X]]|2.20|0.35|
|[[:human_genes:G:GPR98|GPR98]]|2.23|0.37|
|[[:human_genes:P:POLR2E|POLR2E]]|2.24|0.49|
|[[:human_genes:R:RAD54L|RAD54L]]|2.26|0.32|
|[[:human_genes:P:PSME3|PSME3]]|2.27|0.36|
|[[:human_genes:O:OPN1SW|OPN1SW]]|2.27|0.32|
|[[:human_genes:R:RAF1|RAF1]]|2.39|0.26|
|[[:human_genes:S:SF1|SF1]]|2.59|0.36|
|[[:human_genes:S:SPI1|SPI1]]|2.63|0.22|
|[[:human_genes:P:PTAR1|PTAR1]]|2.77|0.22|
|[[:human_genes:U:USP17L17|USP17L17]]|2.87|0.45|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp149|SB203580 25μM R03 exp149]]|0.086|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.074|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp131|L-74142 5μM R03 exp131]]|0.07|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|0.069|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|0.066|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|0.063|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|0.061|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|0.061|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.061|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.059|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp453|B02 10μM R08 exp453]]|0.055|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|0.054|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|0.051|<button type="primary" size="small" modal="13">View</button>|
</modal>


<modal id="1" size="lg" title="SB203580 25μM R03 exp149  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp149_vs_exp538.png?nolink |}}
</modal>

<modal id="2" size="lg" title="BS-181 20μM R08 exp461  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp461_vs_exp538.png?nolink |}}
</modal>

<modal id="3" size="lg" title="L-74142 5μM R03 exp131  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp131_vs_exp538.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Bisphenol F 50μM R08 exp457  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp457_vs_exp538.png?nolink |}}
</modal>

<modal id="5" size="lg" title="CCT251545 20μM R07 exp326  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp326_vs_exp538.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Lapatinib 5μM R01 exp47  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp47_vs_exp538.png?nolink |}}
</modal>

<modal id="7" size="lg" title="IU1-47 25μM R04 exp182  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp182_vs_exp538.png?nolink |}}
</modal>

<modal id="8" size="lg" title="NN-Dimethylsphingosine 2.5μM R08 exp508  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp508_vs_exp538.png?nolink |}}
</modal>

<modal id="9" size="lg" title="WYE-354 6μM R02 exp90  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp538_vs_exp90.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BH1 1μM R04 exp169  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp169_vs_exp538.png?nolink |}}
</modal>

<modal id="11" size="lg" title="B02 10μM R08 exp453  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp453_vs_exp538.png?nolink |}}
</modal>

<modal id="12" size="lg" title="BRD2 inhibitor II 20μM R07 exp341  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp341_vs_exp538.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Cannabidiol 20μM R08 exp466  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp466_vs_exp538.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|sphinganine metabolic process|690.38|SPTLC1,SPTLC2|
|C-palmitoyltransferase activity|517.78|SPTLC1,SPTLC2,SPTSSA|
|serine C-palmitoyltransferase activity|517.78|SPTLC1,SPTLC2,SPTSSA|
|serine C-palmitoyltransferase complex|295.88|SPTLC1,SPTLC2,SPTSSA|
|palmitoyltransferase complex|207.11|SPTLC1,SPTLC2,SPTSSA|
|sphingoid biosynthetic process|172.59|SPTLC1,SPTLC2,SPTSSA|
|sphingosine biosynthetic process|172.59|SPTLC1,SPTLC2,SPTSSA|
|sphingoid metabolic process|138.07|SPTLC1,KDSR,SPTLC2,SPTSSA|
|C-acyltransferase activity|115.06|SPTLC1,SPTLC2,SPTSSA|
|sphingosine metabolic process|115.06|SPTLC1,SPTLC2,SPTSSA|
|diol biosynthetic process|109.01|SPTLC1,SPTLC2,SPTSSA|
|phosphotransferase activity, for other substituted phosphate groups|98.62|DPAGT1,CDIPT,CEPT1|
|diol metabolic process|73.97|SPTLC1,SPTLC2,SPTSSA|
|polyol metabolic process|30.68|SPTLC1,SPTLC2,SPTSSA,SLC5A3|
|alcohol metabolic process|15.24|SPTLC1,SPTLC2,SPTSSA,SCAP,DPAGT1,SLC5A3,MBTPS1|
|lipid biosynthetic process|10.84|SPTLC1,KDSR,CHKA,SPTLC2,ACSL1,SPTSSA,CDIPT,ACACA,PDSS1|
|organic hydroxy compound metabolic process|10.22|SPTLC1,SPTLC2,SPTSSA,SCAP,DPAGT1,SLC5A3,MBTPS1|
|cellular lipid metabolic process|9.39|SPTLC1,KDSR,CHKA,SPTLC2,ACSL1,SPTSSA,SCAP,DPAGT1,CDIPT,ACACA,PDSS1,CEPT1|
|organophosphate biosynthetic process|9.37|SPTLC1,CHKA,SPTLC2,ACSL1,CDIPT,ACACA,CEPT1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>