====== Nocodazole 0.1μM R03 exp94 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Nocodazole%%
    * **<color #00a2e8>Synonyms:</color>** Oncodazole; R17934
    * **<color #00a2e8>CAS #:</color>** 31430-18-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/4122|4122]]
    * **<color #00a2e8>IUPAC:</color>** %%methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
    * **<color #00a2e8>INCHI Key:</color>** KYRVNWMVYQXFEU-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 301.32
    * **<color #00a2e8>Canonical SMILES:</color>** COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C14H11N3O3S
{{:chemogenomics:structures:chem-0013.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** M1404
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Nocodazole
    * **<color #00a2e8>Min:</color>** -9.8754; **<color #00a2e8>Max:</color>** 86.3481
{{:chemogenomics:dose_response:dr_270.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0313    |
| IC30  |0.0403    |
| IC40  |0.0496    |
| IC50  |0.0601    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 03
    * **<color #00a2e8>Dose</color>**: 100nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.3
    * **<color #00a2e8>Numbers of reads</color>**: 10482471


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/179|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Nocodazole_0.1uM_Round-3_exp94.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Nocodazole 0.1μM R03 exp94'>
{{:chemogenomics:cranks_plots:exp94.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:COPE|COPE]]|-3.10|0.40|
|[[:human_genes:S:SART1|SART1]]|-2.86|0.43|
|[[:human_genes:R:RPL36A-HNRNPH2|RPL36A-HNRNPH2]]|-2.78|0.45|
|[[:human_genes:N:NPM3|NPM3]]|-2.72|0.30|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.72|0.48|
|[[:human_genes:N:NAPA|NAPA]]|-2.56|0.35|
|[[:human_genes:N:NOM1|NOM1]]|-2.46|0.35|
|[[:human_genes:M:MUC15|MUC15]]|-2.41|0.35|
|[[:human_genes:V:VPS25|VPS25]]|-2.35|0.42|
|[[:human_genes:M:MDH2|MDH2]]|-2.34|0.35|
|[[:human_genes:A:ATP12A|ATP12A]]|-2.32|0.35|
|[[:human_genes:B:BICD2|BICD2]]|-2.28|0.35|
|[[:human_genes:S:SLC30A1|SLC30A1]]|-2.24|0.35|
|[[:human_genes:C:CDC73|CDC73]]|-2.20|0.42|
|[[:human_genes:Z:ZNF830|ZNF830]]|-2.20|0.35|
|[[:human_genes:B:BOD1L1|BOD1L1]]|-2.18|0.35|
|[[:human_genes:S:SPI1|SPI1]]|-2.14|0.38|
|[[:human_genes:F:FBXW9|FBXW9]]|-2.14|0.35|
|[[:human_genes:H:HCFC1|HCFC1]]|-2.13|0.68|
|[[:human_genes:G:GPX4|GPX4]]|-2.10|0.35|
|[[:human_genes:C:CLEC4C|CLEC4C]]|-2.09|0.35|
|[[:human_genes:R:RFC1|RFC1]]|-2.09|0.50|
|[[:human_genes:C:CENPL|CENPL]]|-2.08|0.38|
|[[:human_genes:C:CD5L|CD5L]]|-2.07|0.35|
|[[:human_genes:S:SCML1|SCML1]]|-2.06|0.35|
|[[:human_genes:I:IQCF3|IQCF3]]|-2.05|0.35|
|[[:human_genes:S:SYVN1|SYVN1]]|-2.04|0.45|
|[[:human_genes:S:SENP1|SENP1]]|-2.02|0.39|
|[[:human_genes:D:DNAJB8|DNAJB8]]|-2.00|0.39|
|[[:human_genes:K:KDM6A|KDM6A]]|-1.99|0.38|
|[[:human_genes:T:TWIST1|TWIST1]]|3.58|<0.01|
|[[:human_genes:D:DFNA5|DFNA5]]|3.59|<0.01|
|[[:human_genes:C:CAMK2B|CAMK2B]]|3.61|<0.01|
|[[:human_genes:L:LRRC72|LRRC72]]|3.69|<0.01|
|[[:human_genes:A:ACTB|ACTB]]|3.75|<0.01|
|[[:human_genes:L:LFNG|LFNG]]|3.77|<0.01|
|[[:human_genes:P:PLEKHA8|PLEKHA8]]|3.79|<0.01|
|[[:human_genes:M:MYL7|MYL7]]|3.82|<0.01|
|[[:human_genes:N:NPY|NPY]]|3.87|<0.01|
|[[:human_genes:I:IQCE|IQCE]]|3.98|<0.01|
|[[:human_genes:I:ICA1|ICA1]]|3.99|<0.01|
|[[:human_genes:C:CPVL|CPVL]]|4.04|<0.01|
|[[:human_genes:T:TTYH3|TTYH3]]|4.11|<0.01|
|[[:human_genes:O:OSBPL3|OSBPL3]]|4.12|<0.01|
|[[:human_genes:I:ITGB8|ITGB8]]|4.14|<0.01|
|[[:human_genes:C:CYTH3|CYTH3]]|4.30|<0.01|
|[[:human_genes:Z:ZWILCH|ZWILCH]]|4.31|<0.01|
|[[:human_genes:F:FNTB|FNTB]]|4.38|<0.01|
|[[:human_genes:S:SGOL1|SGOL1]]|4.52|<0.01|
|[[:human_genes:A:AMZ1|AMZ1]]|4.53|<0.01|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|5.25|<0.01|
|[[:human_genes:M:MAD2L1BP|MAD2L1BP]]|5.51|<0.01|
|[[:human_genes:R:RBM14|RBM14]]|5.54|<0.01|
|[[:human_genes:B:BUB1B|BUB1B]]|5.70|<0.01|
|[[:human_genes:M:MAD2L1|MAD2L1]]|5.89|<0.01|
|[[:human_genes:B:BUB3|BUB3]]|6.01|<0.01|
|[[:human_genes:M:MAD1L1|MAD1L1]]|6.44|<0.01|
|[[:human_genes:Z:ZW10|ZW10]]|6.67|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|7.45|<0.01|
|[[:human_genes:T:TRIP13|TRIP13]]|8.13|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.343|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|0.341|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|0.311|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.286|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.225|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|0.217|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|0.056|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.338|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|0.271|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.118|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|0.064|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|0.06|<button type="primary" size="small" modal="12">View</button>|
</modal>


<modal id="1" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Rotenone 2μM R00 exp33">
{{:chemogenomics:comparison_plots:exp33_vs_exp94.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  SB743921 0.001μM R05 exp244">
{{:chemogenomics:comparison_plots:exp244_vs_exp94.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Nocodazole 0.2μM R00 exp24">
{{:chemogenomics:comparison_plots:exp24_vs_exp94.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  R-DABN 8μM R03 exp111">
{{:chemogenomics:comparison_plots:exp111_vs_exp94.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Vinblastine 0.015μM R05 exp208">
{{:chemogenomics:comparison_plots:exp208_vs_exp94.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp64_vs_exp94.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp94.png?nolink |}}
</modal>

<modal id="8" size="lg" title="DABN racemic mixture R03 exp93  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp93_vs_exp94.png?nolink |}}
</modal>

<modal id="9" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp256_vs_exp94.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BI-6727 0.04μM R03 exp98  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp94_vs_exp98.png?nolink |}}
</modal>

<modal id="11" size="lg" title="BI-2536 0.02μM R03 exp96  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp94_vs_exp96.png?nolink |}}
</modal>

<modal id="12" size="lg" title="GSK461364A 0.1μM R03 exp126  vs  Nocodazole 0.1μM R03 exp94">
{{:chemogenomics:comparison_plots:exp126_vs_exp94.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitotic spindle checkpoint signaling|157.90|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|spindle assembly checkpoint signaling|157.90|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|mitotic spindle assembly checkpoint signaling|157.90|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|negative regulation of mitotic sister chromatid segregation|142.62|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|negative regulation of mitotic sister chromatid separation|142.62|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|negative regulation of chromosome segregation|133.98|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|negative regulation of metaphase/anaphase transition of cell cycle|133.98|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|negative regulation of chromosome separation|133.98|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|spindle checkpoint signaling|133.40|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,BUB1B,ZWILCH|
|negative regulation of mitotic metaphase/anaphase transition|124.80|TRIP13,KNTC1,ZW10,MAD1L1,MAD2L1,BUB1B,ZWILCH|
|negative regulation of sister chromatid segregation|124.80|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,BUB1B,ZWILCH|
|negative regulation of nuclear division|96.12|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH|
|regulation of mitotic sister chromatid segregation|93.85|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH|
|regulation of mitotic sister chromatid separation|76.23|TRIP13,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH|
|regulation of chromosome separation|68.14|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH|
|regulation of mitotic metaphase/anaphase transition|62.10|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB|
|regulation of metaphase/anaphase transition of cell cycle|60.07|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB|
|regulation of sister chromatid segregation|53.66|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB|
|regulation of chromosome segregation|43.86|TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB|
</modal>